“…A Cyt c reduction assay which is dependent on the presence of NADPH as a primary electron donor, a pyridine nucleotide reductase, an electron carrier, and Cyt c as an electron acceptor, has also been used to characterize Fd or Fd-like proteins as an electron carrier (1,4,11,23). Using the Cyt c reduction assay system, a non-heme iron containing protein which transfers electrons from either NADPH or NADH to Cyt c has been identified in extracts prepared from young maize roots.…”
“…A Cyt c reduction assay which is dependent on the presence of NADPH as a primary electron donor, a pyridine nucleotide reductase, an electron carrier, and Cyt c as an electron acceptor, has also been used to characterize Fd or Fd-like proteins as an electron carrier (1,4,11,23). Using the Cyt c reduction assay system, a non-heme iron containing protein which transfers electrons from either NADPH or NADH to Cyt c has been identified in extracts prepared from young maize roots.…”
“…For the same species, and using antibodies to measure Fd, Böhme (1977) reported 475 Chl/Fd (2 Fd/P700) and Bohme (1978) reported a ratio of 250 Chl/Fd (3 Fd/PSI). However, Matson and Kimura (1975) reported a ratio of only 33 Chl/Fd in S. oleracea and 11 Chl/Fd in Petroselinum crispum, also using antibodies to measure Fd. It is unclear why the experiment by Matson and Kimura (1975) resulted in an order of magnitude difference with respect to the other experiments.…”
Section: Concentrations and Amountsmentioning
confidence: 99%
“…However, Matson and Kimura (1975) reported a ratio of only 33 Chl/Fd in S. oleracea and 11 Chl/Fd in Petroselinum crispum, also using antibodies to measure Fd. It is unclear why the experiment by Matson and Kimura (1975) resulted in an order of magnitude difference with respect to the other experiments. Assuming 2 Fd/P700 (which would result in 222 Chl/Fd with our parameter set), this leads to an Fd content of 1.28×10 −6 mol m −2 .…”
Dynamic photosynthesis under a fluctuating environment: a modelling-based analysis, 282 pages.PhD thesis, Wageningen University, Wageningen, NL (2017) With references, with summary in English
Die quantitative Bestimmung des Rubredoxingehaltes in Bakterienrohextrakten von Acinetobacter calcoaceticus erfolgte mit Hilfe einer immunologischen Methode. Da diskelektrophoretisch homogenes, natives Rubredoxin bei Kaninchen keine humorale Immunantwort hervorruft, wurde die Immunogenitiit des Antigens durch Vernetzen mit Glutaraldehyd erhoht. Die gegen vernetztes Rubredoxin in Kaninchen gebildeten Antikorper priizipitieren sowohl das vernetzte als auch das native Rubredoxin. Eine geringe Reaktion zeigte das gewonnene Antiserum auch gegenuber Ferredoxin aue Spinatbliittern. Hingegen iet mit Adrenodoxin aus Nebennierenmitochondrien keine Prazipitation zu beobachten. Die durch Rubredoxin-Reductase BUS Ac. calcoaceticus katalysierte Rubredoxin-abhiingige Reduktion von Cytochrom c wird durch das gewonnene Antiserum gehemmt.Zur quantitativen Bestimmung von Rubredoxin in Zellrohextrakten konnte ein Radioimmuntest mit einer Nachweisgrenze bei 0,02 nmol Rubredoxin/ml Probe aufgebaut werden. Der Rubredoxingehalt in Rohextrakten von Ac. culcoaceticua betragt nach Wachstum auf n-Alkanen 4 lo-" mol/mg Bakterienprotein.
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