Immunological properties of gold nanoparticles

Дыкман, Л. А.; Khlebtsov, Nikolai G.

doi:10.1039/c6sc03631g

Cited by 194 publications

(124 citation statements)

References 215 publications

(192 reference statements)

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“…In contrast to PVP-GNPs, PEG-GNPs reduced the capacity of PHA-stimulated PBMNC to produce proinflammatory cytokines (IL-1β, TNF-α and IL-6) significantly, and upregulated their capacity to produce anti-inflammatory cytokine IL-4, involved in the Th2 polarisation of immune response [65]. This is line with the expected immunological effects of PEG-GNPs [66], and could be explained by the anti-inflammatory effects of GNPs on APC, such as DC [35]. The differences in immunological effects of GNPs stabilised with different stabilisation agents, could be a consequence of different levels and the mechanisms of their internalisation by APC.…”

Section: Discussionsupporting

confidence: 72%

The Effect of Stabilisation Agents on the Immunomodulatory Properties of Gold Nanoparticles Obtained by Ultrasonic Spray Pyrolysis

et al. 2019

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Gold nanoparticles (GNPs) have been investigated extensively as drug carriers in tumour immunotherapy in combination with photothermal therapy. For this purpose, GNPs should be stabilised in biological fluids. The goal of this study was to examine how stabilisation agents influence cytotoxicity and immune response in vitro. Spherical GNPs, 20 nm in size, were prepared by ultrasonic spray pyrolysis (USP). Three types of stabilising agents were used: sodium citrate (SC), polyvinyl-pyrrolidone (PVP), and poly-ethylene glycol (PEG). Pristine, non-stabilised GNPs were used as a control. The culture models were mouse L929 cells, B16F10 melanoma cells and human peripheral blood mononuclear cells (PBMNCs), obtained from healthy donors. Control SC- and PEG-GNPs were non-cytotoxic at concentrations (range 1–100 µg/mL), in contrast to PVP-GNPs, which were cytotoxic at higher concentrations. Control GNPs inhibited the production of IFN-ϒ slightly, and augmented the production of IL-10 by PHA-stimulated PBMNC cultures. PEG-GNPs inhibited the production of pro-inflammatory cytokines (IL-1, IL-6, IL-8, TNF-α) and Th1-related cytokines (IFN-ϒ and IL-12p70), and increased the production of Th2 cytokines (IL-4 and IL-5). SC-PEG inhibited the production of IL-8 and IL-17A. In contrast, PVP-GNPs stimulated the production of pro-inflammatory cytokines, Th1 cytokines, and IL-17A, but also IL-10. When uptake of GNPs by monocytes/macrophages in PBMNC cultures was analysed, the ingestion of PEG- GNPs was significantly lower compared to SC- and PVP-GNPs. In conclusion, stabilisation agents modulate biocompatibility and immune response significantly, so their adequate choice for preparation of GNPs is an important factor when considering the use of GNPs for application in vivo.

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Section: Discussionsupporting

confidence: 72%

The Effect of Stabilisation Agents on the Immunomodulatory Properties of Gold Nanoparticles Obtained by Ultrasonic Spray Pyrolysis

et al. 2019

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“…Additionally, we analyzed the expression of tumor necrosis factor alpha (TNF‐α), an essential indicator characterizing the host inflammatory response . Wounds treated with AuNRs@pLAMA/pFEMA‐50 under 808 nm NIR irradiation exhibited the lowest expression level of TNF‐α, similar to a healthy tissue, compared with other experimental and control groups (Figure a,c).…”

mentioning

confidence: 99%

A Biomimetic Non‐Antibiotic Approach to Eradicate Drug‐Resistant Infections

et al. 2018

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The chronic infections by pathogenic Pseudomonas aeruginosa (P. aeruginosa) remain to be properly addressed. In particular, for drug‐resistant strains, limited medication is available. An in vivo pneumonia model induced by a clinically isolated aminoglycoside resistant strain of P. aeruginosa is developed. Tobramycin clinically treating P. aeruginosa infections is found to be ineffective to inhibit or eliminate this drug‐resistant strain. Here, a newly developed non‐antibiotics based nanoformulation plus near‐infrared (NIR) photothermal treatment shows a remarkable antibacterial efficacy in treating this drug‐resistant pneumonia. The novel formulation contains 50–100 nm long nanorods decorated with two types of glycomimetic polymers to specifically block bacterial LecA and LecB lectins, respectively, which are essential for bacterial biofilm development. Such a 3D display of heteromultivalent glycomimetics on a large scale is inspired by the natural strengthening mechanism for the carbohydrate–lectin interaction that occurs when bacteria initially infects the host. This novel formulation shows the most efficient bacteria inhabitation and killing against P. aeruginosa infection, through lectin blocking and the near‐infrared‐light‐induced photothermal effect of gold nanorods, respectively. Collectively, the novel biomimetic design combined with the photothermal killing capability is expected to be an alternative treatment strategy against the ever‐threatening drug‐resistant infectious diseases when known antibiotics have failed.

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“…According to the colorimetric determination of carbohydrates (phenol-sulfuric acid reaction, see the Experimental section for details) in the samples of glyco-GNPs 3 and 4, which were puri- fied from the excess of Ara 6 glycosides 1 and 2 by centrifugation, there are no more than 100 glycoside molecules on each nanoparticle on average. Spherical citrate-stabilized GNPs with 15 nm average diameter (which are often considered as standard GNPs for immunochemical studies due to ease of preparation and conjugation with ligands [48,56,57] and have successfully been used in our previous studies [56,57,77,78,95]) were chosen for the experiments. This is because GNPs of this size, unlike much smaller GNPs (e.g., around 2 nm in diameter), which are often derivatized in situ [32,39,46,48], can be easily prepared in advance and kept for further conjugation with a desired ligand.…”

Section: Glyco-gnpsmentioning

confidence: 99%

“…The use of gold nanoparticles (GNPs) [24][25][26] as carriers for glyco-epitopes is becoming an important alternative [15, to the traditional conjugation with proteins, synthetic polymers and other carriers [15,17,38,[49][50][51][52][53][54][55]. Immunological properties of GNPs [25,56,57] and their use in vaccine development [58] have recently been reviewed. Glyco-gold nanoparticles (glyco-GNPs) bearing residues of tumor-associated monosaccharide Tn [37] or disaccharide Thomsen-Friedenreich (TF) [35] antigens, a tetrasaccharide of the Streptococcus pneumonia type-14 capsular polysaccharide [33,34,47], or lipopolysaccharides of Burkolderia mallei [42] have been shown to be promising vaccine candidates.…”

Section: Introductionmentioning

confidence: 99%

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Burygin¹,

Abronina²,

Podvalnyy³

et al. 2020

Beilstein J. Nanotechnol.

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A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while there was only a weak response to M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.

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Immunological properties of gold nanoparticles

Abstract: This review summarizes what is known about the application of gold nanoparticles as an antigen carrier and adjuvant in immunization for the preparation of antibodies in vivo and evaluating their potential for the development of effective vaccines.

Cited by 194 publications

References 215 publications

The Effect of Stabilisation Agents on the Immunomodulatory Properties of Gold Nanoparticles Obtained by Ultrasonic Spray Pyrolysis

The Effect of Stabilisation Agents on the Immunomodulatory Properties of Gold Nanoparticles Obtained by Ultrasonic Spray Pyrolysis

A Biomimetic Non‐Antibiotic Approach to Eradicate Drug‐Resistant Infections

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

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