Immunological methods in plant hormone research

Beale, Michael H.

doi:10.1016/s0167-7306(08)60483-4

Cited by 5 publications

(6 citation statements)

References 142 publications

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“…Several analytical techniques have been used in plant hormone analysis ( − ). While methods based on ultraviolet or electron capture detectors are almost abandoned, indirect methods such as enzyme-linked immunosorbent assays are still used frequently ( , ). Recently, determination of ABA by capillary electrophoresis with laser-induced fluorescence detection has been achieved ().…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

Durgbanshi

Arbona

Pozo

et al. 2005

J. Agric. Food Chem.

275

181

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A rapid multiresidue method to quantify three different classes of plant hormones has been developed. The reduced concentrations of these metabolites in real samples with complex matrixes require sensitive techniques for their quantification in small amounts of plant tissue. The method described combines high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Deuterium-labeled standards were added prior to sample extraction to achieve an accurate quantification of abscisic acid, indole-3-acetic acid, and jasmonic acid in a single run. A simple method of extraction and purification involving only centrifugation, a partition against diethyl ether, and filtration was developed and the analytical method validated in four different plant tissues, citrus leaves, papaya roots, barley seedlings, and barley immature embryos. This method represents a clear advantage because it extensively reduces sample preparation and total time for routine analysis of phytohormones in real plant samples.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

Durgbanshi

Arbona

Pozo

et al. 2005

J. Agric. Food Chem.

275

181

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“…A number of sensitive and specific methods have been developed for quantification of individual plant hormones. These include GC–MS, high‐performance liquid chromatography (HPLC) and liquid chromatography‐mass spectrometry (LC–MS) (see Crozier and Moritz, 1999), as well as indirect methods such as enzyme‐linked immunosorbent assays (ELISA) (see Beale, 1999).…”

Section: Introductionmentioning

confidence: 99%

A method for profiling classes of plant hormones and their metabolites using liquid chromatography‐electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds

et al. 2003

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SummaryA highly selective and sensitive method for the simultaneous analysis of several plant hormones and their metabolites is described. The method combines high-performance liquid chromatography (HPLC) with positive and negative electrospray ionization-tandem mass spectrometry (ESI±MS/MS) to quantify a broad range of chemically and structurally diverse compounds. The addition of deuterium-labeled analogs for these compounds prior to sample extraction permits accurate quanti®cation by multiple reaction monitoring (MRM). Endogenous levels of abscisic acid (ABA), abscisic acid glucose ester (ABA-GE), 7H -hydroxyabscisic acid (7 H -OH-ABA), phaseic acid (PA), dihydrophaseic acid (DPA), indole-3-acetic acid (IAA), indole-3-aspartate (IAAsp), zeatin (Z), zeatin riboside (ZR), isopentenyladenine (2iP), isopentenyladenosine (IPA), and gibberellins (GA) 1 , GA 3 , GA 4 , and GA 7 were determined simultaneously in a single run. Detection limits ranged from 0.682 fmol for Z to 1.53 pmol for ABA. The method was applied to the analysis of plant hormones and hormonal metabolites associated with seed dormancy and germination in lettuce (Lactuca sativa L. cv. Grand Rapids), using extracts from only 50 to 100 mg DW of seed. Thermodormancy was induced by incubating seeds at 338C instead of 238C. Germinating seeds transiently accumulated high levels of ABA-GE. In contrast, thermodormant seeds transiently accumulated high levels of DPA after 7 days at 338C. GA 1 and GA 3 were detected during germination, and levels of GA 1 increased during early postgerminative growth. After several days of incubation, thermodormant seeds exhibited a striking transient accumulation of IAA, which did not occur in seeds germinating at 238C. We conclude that hormone metabolism in thermodormant seeds is surprisingly active and is signi®cantly different from that of germinating seeds.

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“…For the usage in a practical milk-progesterone assay kit the antibodies which do not react with other sex hormones had to be prepared. Because the hapten was linked to BSA at position 11α, higher cross-reactivity with steroid structures modified on carbon 11 and 19 localized in the area of coupling was predictable (Erlanger et al, 1957;Beale, 1999); however, these steroids should not interfere with progesterone in milk.…”

Section: Standardsmentioning

confidence: 99%

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

Simerský¹,

Swaczynová²,

Morris³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:Effective reproductive management is a crucial concern in the dairy industry and missed oestrus and late recognition of unsuccessful insemination can lead to substantial financial loss. Progesterone levels change in a predictable manner with progress of the oestrus cycle and with the onset of pregnancy. We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk. The method is based on the use of polyclonal antibodies raised against 11α-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3' ,5,5'-tetramethylbenzidine. The method has proved to be a cost effective, sensitive and easy to perform alternative to currently available methods of oestrus and pregnancy detection. The practical feasibility of the method has been proven under dairy farm conditions.

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Immunological methods in plant hormone research

Cited by 5 publications

References 142 publications

Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

A method for profiling classes of plant hormones and their metabolites using liquid chromatography‐electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds

Development of an ELISA-based kit for the on-farm determination of progesterone in milk

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