Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis

Abstract

Human neurocysticercosis, due to infection of the central nervous system by cysticerci of Taenia solium, is a severe form of neurologic disease occurring in Central and South America. Specific proteins from scolex antigen from cysticerci were purified by polyacrylamide gel electrophoresis and electroelution and recognized in Western blots by antibodies present in sera from patients with neurocysticercosis. The proteins appeared as 13-, 17-, and 26-kDa bands on Coomassie blue-stained gels and proved to be specific to cysticerci of T. solium. No cross-reactivity with sera from patients with taeniasis or hydatidosis was observed. Enzyme-linked immunosorbent assay using the purified proteins of 13, 17, and 26 kDa demonstrated rates of 53%, 88%, and 100% specificity, respectively, at the cutoff serum dilution of 1:32 for the specific immunodiagnosis of human neurocysticercosis.

Neurocysticercosis: relationship between the developmental stage of metacestode present and the titre of specific IgG in the cerebrospinal fluid

“…The immunological studies are usually based on the detection in sera of antibodies toward the causative agent (Taenia solium) or of antigens from the same cestode. The antibody-detection assays used have been based on recombinant proteins or natural antigens from T. solium (Rosas et al, 1986;Tsang et al, 1989;Ito et al, 1998;Ko and Ng, 1998;D'Souza and Hafez, 1999;Ev et al, 1999;Plancarte et al, 1999;Hancock et al, 2003), T. crassiceps (Garcia et al, 1995;Peralta et al, 2002) or T. saginata (Ferrer et al, 2003).…”

Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

“…6 Thus, the disease may result primarily from the host inflammatory response to dying parasites. 7 Many efforts have been made to use homologous antigens, [8][9][10][11][12][13][14] T. crassiceps antigens and synthetic peptides, [15][16][17] a phage display peptide library, 18 and recombinant T. solium antigens in the immunodiagnosis of cysticercosis. [19][20][21][22] Host-parasite interaction is poorly understood in NCC.…”

Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine proteinases of adult fluke

“…In the present work, two cysteine proteinases of 28 and 34 kDa were purified, by means of preparative electrophoresis and electroelution in polyacrylamide gels. This method has been previously used in the isolation of antigens of diagnostic utility in fasciolosis [30] and other parasitic diseases [12].…”