Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

Abstract

Abstract. To detect IgG antibodies to Taenia solium, a controlled double-blind study was conducted using 91 coded cerebrospinal fluid samples from patients with neurocysticercosis (NCC) and other neurologic disorders. Samples were tested in an enzyme-linked immunosorbent assay (ELISA) using metacestode excretion/secretion antigens. The results were correlated with data from medical records on the diagnosis of NCC (based on computed tomography and magnetic resonance imaging criteria) and other neurologic disorders. The ELISA results were positive in 22 of the 24 cases with active NCC. In contrast, six cases with calcified cysts (inactive NCC), as well as one case in a transitional stage, were negative. One case with a calcified granuloma and another with a granuloma plus calcifications (classified as inactive NCC) had positive results. The remaining negative results corresponded to other neurologic disorders (58 cases). The results of the ELISA showed a significant difference between active and inactive NCC (P ‫ס‬ 0.0034).

Utility of cysticercus fasciolaris antigen in Dot ELISA for the diagnosis of neurocysticercosis

“…[3,4] We have used membrane antigen of cysticercus fasciolaris (larval stage of Taenia taeniaeformis pathogenic in rat) in anti-IgG and anti-IgM ELISA for the immunodiagnosis of NC. [5] In the current study, we have designed and evaluated a Dot ELISA using membrane extract of cysticercus fasciolaris antigen for immunodiagnosis of neurocysticercosis.…”

Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance

“…Immunological tests are more successful with CSF samples, but results are dependent upon the activity of the infection. Inactive cysticerci are usually associated with very low reactivity (162,192). Immunofluorescence and complement fixation (CFA) tests are much less sensitive and specific than ELISA and WB, especially with use of purified antigens (30).…”

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

“…Rodriguez-Hidalgo et al (2006) analyzed serum of infected swine and obtained high reactivity on 50 and 39-42 kDa protein bands. As reported by Molinari et al (2002), the indirect ELISA results showed that samples from patients with alive cysticercus had higher absorbance than samples from patients whose cysts were calcified or in transitional stages, but this distinction could not be made by immunoblotting. Although different immunodominant proteins were found in the EITB, any band was able to discriminate the different forms of neurocysticercosis.…”

“…Therefore, the use of this heterologous antigen may contribute for seroepidemiologic studies to evaluate the complex taeniasis/cysticercosis (Vaz et al, 1996;, Sciutto et al, 2000, Ito, 2000. Furthermore, studies have shown that immunoassays were able to detect whether the patients presented alive or inactive cysticerci (Molinari et al, 2002, Barcelos et al, 2005. In order to evaluate and confirm the epidemiological data of neurocysticercosis in the State of Paraná, Brazil, C. longicollis crude extract was used as heterologous antigen of C. cellulosae in ELISA and EITB assay of patients with different (Sotelo et al, 1988) diagnosed through neuroimages features.…”

“…The search for antigens is rare in neurocysticercosis diagnosis (Flisser and Gyorkos, 2007) and immunodiagnosis is based, primarily, on the detection of antibodies (Schantz and Tsang, 2003) by ELISA and EITB (Dua and Aneja, 2006;Schantz, 2006). Studies have shown it is possible by ELISA and using different antigens to discriminate between active and inactive NCC, i.e., when the cysticercus is alive or inert, respectively (Molinari et al, 2002;Barcelos et al, 2005). The aim of this work was to compare the experimental results obtained by ELISA and immunoblot assays using C. longicollis crude antigens and sera and cerebrospinal fluid (CSF) from patients with active, inactive NCC or with cysts in transitional phase, i.e., in degeneration, from the State of Paraná, Brazil.…”