Immunological Cross-Reactivity of the Major Allergen from Perennial Ryegrass <i>(Lolium perenne</i><i>)</i>, <i>Lol</i> <i>p</i> I, and the Cysteine Proteinase, Bromelain

Abstract

Antibodies prepared in rabbits against the major allergen from ryegrass (Lolium perenne), Lol p I, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. Deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. The results indicate that the carbohydrate residues from these allergens play an important role in cross-reactions found between them and possibly those from other species.

Comparison of Gingival Crevicular Fluid Sampling Methods in Patients With Severe Chronic Periodontitis

“…Antibodies (immunoglobulin Y [IgY]) anti‐HRgpA were raised in chickens as described by Pike et al 24 The IgY specific for the Rgp catalytic domain (identical in RgpA and RgpB) were purified by affinity chromatography using immobilized RgpB. Anti‐RgpB (clone 25G8.A8.G6) mouse monoclonal antibodies (mAb) was produced on site in a monoclonal facility ††† .…”

In vitro hymenoptera venom allergy diagnosis: improved by screening for cross‐reactive carbohydrate determinants and reciprocal inhibition

“…Rape pollen and bromelain are representative CCD‐containing allergens, the latter rarely inducing allergic symptoms (9, 17, 18). Horseradish peroxidase was chosen because it is a ‘multivalent’ glycoprotein (seven glycan chains in contrast to bromelain, which has only one) and because there is no natural exposure to the human organism (19).…”

Sensitization to cross‐reactive carbohydrate determinants and the ubiquitous protein profilin: mimickers of allergy

“…Cross-reactivity, however, extends beyond the protein level. Characteristic a(1,3)-fucose (linked to the proximal N-acetyl glucosamine) and/or b(1,2)-xylose (linked to the core mannose) bearing carbohydrate moieties of pollen and plant food glycoproteins, generally referred to as cross-reactive carbohydrate determinants (CCDs), have also been identified as highly cross-reactive IgE-binding structures [1,4,[9][10][11][12][13][14]. The prevalence of anti-CCD IgE has been estimated to be 10-15% in patients with grass pollen allergy [15] and increases up to 60% in patients with concomitant sensitization to pollen from trees, grasses and weeds [16].…”