Immunological Comparisons of Teliospores of Two Wheat Bunt Fungi, Tilletia Species, Using Monoclonal Antibodies and Antisera

Detection of Tilletia caries, Causal Agent of Common Bunt of Wheat, by ELISA and PCR

“…Our results clearly show that there is a very close serological relatedness between the two fungi, as the antibodies against T. caries also reacted strongly with extracts of two T. controversa isolates. This is in close agreement with the results of a study by Banowetz et al. (1984) who could not distinguish the two species with polyclonal or monoclonal antibodies directed against surface structures of the teliospores.…”

Detection of Tilletia controversa using immunofluorescent monoclonal antibodies

“…Such as by comparing polyclonal antisera and monoclonal antibodies, Banowetz et al . () reported that both T. controversa and T. caries teliospores reacted with polyclonal sera bound the fluorescent probe to the same degree, and each monoclonal antibody derived in their study reacted strongly with both fungi, which indicates the close antigenic relationship of these species. Zhang et al .…”

“…Previous attempts have been made to identify T. controversa and other closely related species using immunologic techniques; however, only unsatisfactory results were achieved. Such as by comparing polyclonal antisera and monoclonal antibodies, Banowetz et al (1984) reported that both T. controversa and T. caries teliospores reacted with polyclonal sera bound the fluorescent probe to the same degree, and each monoclonal antibody derived in their study reacted strongly with both fungi, which indicates the close antigenic relationship of these species. Zhang et al (2007) developed an ELISA and demonstrated that polyclonal antibodies derived from fine powder of the teliospores of T. controversa failed to differentiate between the intact teliospores of T. controversa, T. caries and Tilletia foetida.…”

Rapid methods for diagnosis of soil‐borne plant pathogens

“…Most of those raised have been used largely for diagnosis and taxonomic purposes in plant pathology (e.g. Iannelli et al, 1983;Banowetz et al, 1984;Benhamou et al, 1984;Hardham et al, 1985;Clarke et al, 1986;Fox & Hahne, 1988;Dewey et al, 1989;Estrada-Garcia et al, 1989). MCAs have also been used in basic and applied research on the fine structure of pathogens (Day et al, 1986;Hardham et d., 1985) and detection of their toxins (Benhamou et al, 1985a(Benhamou et al, , 1985b.…”