Detection of <i>Tilletia controversa</i>
 using immunofluorescent monoclonal antibodies

Gao, Li; Chen, Feng; Li, B.; Liu, T.; Liu, B.; Chen, W.

doi:10.1111/jam.12703

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…In another analysis, an abundant 116-kD polypeptide of undefined nature was detected in extracts from teliospores of T. controversa exclusively (Banowetz and Doss 1994 ). Numerous research groups tried to develop immunoassays to differentiate the three Tilletia species, but were unable to create assays specific to all three of them (Eibel et al 2005 ; Zhang et al 2007 ; Gao et al 2015 ). Innovative proteomic analyses of Tilletia species were performed by Li et al ( 2018 ) using the isobaric tags for relative and absolute quantitation (iTRAQ) technique to explore proteomic differences among T. controversa , T. caries , and T. laevis by detecting relative quantities of specific proteins between the pathogens and Pandey et al ( 2018 and 2019) applied tandem mass spectrometry to identify pathogenicity or virulence-related proteins of T. indica .…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores

Förster

Sedaghatjoo

Maier

et al. 2022

Appl Microbiol Biotechnol

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The fungal genus Tilletia includes a large number of plant pathogens of Poaceae. Only a few of those cause bunt of wheat, but these species can lead to significant yield losses in crop production worldwide. Due to quarantine regulations and specific disease control using appropriate seed treatments for the different disease agents, it is of high importance to distinguish Tilletia caries and Tilletia laevis as causal agents of common bunt accurately from Tilletia controversa, the causal agent of the dwarf bunt. Several studies have shown that matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a useful tool to differentiate closely related fungal species. The aim of this study was to assess whether MALDI-TOF MS analysis is able to distinguish specimens of the three closely related pathogens T. caries, T. laevis, and T. controversa and whether it may constitute an alternative method to the morphology-based identification or germination tests. Spectral data are available via ProteomeXchange with identifier PXD030401. Spectra-based hierarchical cluster analysis (HCA) and discriminant analysis of principal components (DAPC) of the obtained mass spectra showed two main clusters. One cluster included specimens of T. controversa, whereas the second cluster comprised T. laevis and T. caries specimens. Even though main spectral profiles (MSPs) for species identification are missing, MALDI-TOF MS has proven to be a useful method for distinguishing between T. controversa and the two causal agents of common bunt, using direct analysis of teliospores, but was unable to separate T. caries and T. laevis species. Key points • MALDI-TOF MS was developed to classify Tilletia species causing bunt of wheat. • Best results were achieved when combining HCA and DAPC analysis. • The method resulted in an accuracy of 98.51% testing 67 Tilletia specimens. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores

Förster

Sedaghatjoo

Maier

et al. 2022

Appl Microbiol Biotechnol

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iTRAQ-Based Proteomic Analysis of Wheat Bunt Fungi Tilletia controversa, T. caries, and T. foetida

Wei

Gao

et al. 2018

Curr Microbiol

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This is the first study of proteomics of wheat bunt fungi Tilletia controversa (TCK), T. caries (TCT), and T. foetida (TFL) using the iTRAQ technique. Based on the relative quantities of specific proteins between each two pathogens, we found 50 up-regulated and 80 down-regulated protein genes in TCK compared to TFL, 62 up-regulated and 82 down-regulated protein genes in TCT compared to TFL, 47 up-regulated and 30 down-regulated protein genes in TCK compared to TCT, and there were 1 protein of up-regulated and 4 proteins of down-regulated in the three pairs. These protein data could be of great value for exploring the key proteins which play an important role in the interactions of these pathogens with their host. Some of them could be valuable for differentiating the three pathogens with monoclonal antibodies produced by the specific proteins and may enable in-site detection of the pathogens and performing routine monitoring as a diagnostic assay in wheat shipments.

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Detection of Tilletia caries, Tilletia laevis and Tilletia controversa wheat grain contamination using loop-mediated isothermal DNA amplification (LAMP)

Pieczul

Perek

Kubiak

2018

Journal of Microbiological Methods

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Detection of Tilletia controversa using immunofluorescent monoclonal antibodies

Abstract: This method could be used for the on-site identification of T. controversa teliospores in the near future and will help in selecting fungicides to control dwarf bunt of wheat as further technical developments are achieved.

Cited by 5 publications

References 34 publications

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores

Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores

iTRAQ-Based Proteomic Analysis of Wheat Bunt Fungi Tilletia controversa, T. caries, and T. foetida

Detection of Tilletia caries, Tilletia laevis and Tilletia controversa wheat grain contamination using loop-mediated isothermal DNA amplification (LAMP)

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