Immunological characterization of honey major protein and its application

Won, Se−Ra; Li, Chunying; Kim, Jang-Won; Rhee, Hae-Ik

doi:10.1016/j.foodchem.2008.08.082

Cited by 56 publications

(43 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to the production decline of A. cerana honey and to a growing demand for local and traditional products, this honey has attained higher market prices than that produced by A. mellifera, generally being three-to five-times more expensive (Won, Li, Kim, & Rhee, 2009).…”

Section: Introductionmentioning

confidence: 99%

Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication

Soares

Grazina

Mafra

et al. 2018

Food Research International

View full text Add to dashboard Cite

Honey can be produced by different species of honeybees, with two being of economic importance due to their use in apiculture, namely Apis mellifera (known as European honeybee) and Apis cerana (known as Asian honeybee). Due to the decline of the wild populations of the Asian honeybee, this honey generally attains much higher market value, being prone to adulteration. This work aims at proposing new tools, based on the use of molecular markers, for the entomological authentication of honey. To this end, new species-specific primers were designed targeting the tRNA-cox2 intergenic region and allowing the detection of A. cerana DNA by qualitative polymerase chain reaction (PCR). Additionally, a novel real-time PCR method with high resolution melting analysis was developed to target the 16S rRNA gene of both bee species, allowing their discrimination in different clusters. The proposed methodologies were further applied with success in the authentication of Asian and European honey samples by the identification of honeybee DNA, demonstrating the usefulness of these simple and cost-effective new approaches.

show abstract

Section: Introductionmentioning

confidence: 99%

Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication

Soares

Grazina

Mafra

et al. 2018

Food Research International

View full text Add to dashboard Cite

show abstract

“…In Shen Nong's Herbal Classic and Compendium of Materia Medica (Lee and others ), A. cerana honey has been recorded as the medicine. Recently, fundamental researches showed that A. cerana honey is of the finest quality in moisture, sugars, and proteins (Joshi and others ; Won and others ; Won and others ). The main bioactive substances of honey are a wide range of phenolic compounds: gallic acid, caffeic acid, p ‐coumaric acid, quercetin, kaempferol, and so on (M Alvarez‐Suarez and others ).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effects of the Honey of Apis cerana Fabricius on Bromobenzene‐Induced Liver Damage in Mice

Zhao

Cheng

et al. 2018

Journal of Food Science

View full text Add to dashboard Cite

Apis cerana honey (honey of Apis cerana Fabricius), widely distributed in the mountain areas of East Asia, has not been studied fully. The hepatoprotective activity of A. cerana honey was evaluated against bromobenzene-induced liver damage in mice. In high dose, A. cerana honey can significantly alleviate liver injury, as is indicated by the depressed levels of serum alanine aminotransferase (ALT) (59.13%) and aspartate aminotransferase (AST) (79.71%), the inhibited malondialdehyde (MDA) content (63.30%), the elevated activities of superoxide dismutase (SOD) (73.12%) and glutathione-Px (57.24%), and the decreased expression of Transforming growth factor β1 (51.83%) induced by bromobenzene (P < 0.05). The quantitative analysis of twelve major constituents (1 to 12) of A. cerana honey was executed by high performance liquid chromatography-diode array detector. The results indicate that treatment with A. cerana honey can prevent bromobenzene-induced hepatic damage in mice. Polyphenols might be the bioactive substances attributed to its antioxidant properties and intervention of oxidative stress.

show abstract

“…The importance of understanding the composition of honey from a human health point of view is also valuable. Furthermore, honey was found to be a suitable alternative for healing wounds and burns, and a product that can be used in oral health treatments (Molan, 2001; Lusby et al ., 2002; Gallardo‐Chacon et al ., 2008; Lay‐flurrie, 2008 ; Won et al ., 2009). It also has a potential role in cancer care and shows antimicrobial properties (Bardy et al ., 2008).…”

Section: Introductionmentioning

confidence: 99%

Palynological and physicochemical data characterisation of honeys produced in the Entre‐Douro e Minho region of Portugal

Feás

Pires

Estevinho

et al. 2010

Int J of Food Sci Tech

View full text Add to dashboard Cite

SummaryHoney legislation has been addressed to establish the minimum marketing level of the product and the need for consumer protection through correct denominations. Research oriented toward assessment of floral origin and physicochemical properties may increase the commercial value of these products. The characteristics of thirty-one honeys produced in the Entre-Douro e Minho region in Portugal were studied. Pollen features and some physicochemical parameters (moisture, ash, pH, free acidity, electrical conductivity, hydroxymethylfurfural contain, apparent sucrose, reducing sugars and diastase activity) were determined. The samples were found to meet international honey specifications. The present study found a linear regression between the ash content of honeys and their specific conductivity. Five samples are listed as Eucalyptus honey, one sample as Citrus honey, and twenty-five samples as multifloral honeys. Of the total, 87.1% exceeded the quality parameters and should be labelled as 'virgin' honey.

show abstract

Immunological characterization of honey major protein and its application

Cited by 56 publications

References 16 publications

Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication

Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication

Hepatoprotective Effects of the Honey of Apis cerana Fabricius on Bromobenzene‐Induced Liver Damage in Mice

Palynological and physicochemical data characterisation of honeys produced in the Entre‐Douro e Minho region of Portugal

Contact Info

Product

Resources

About