Immunological characterization of an immunomodulatory epitope in S-antigen/arrestin with a sequence motif common to tumor necrosis factor α

Anti-retinal autoantibodies in experimental ocular and systemic toxoplasmosis

“…Using an experimental rabbit model of systemic and ocular toxoplasmosis [33], and an established autoantibody ELISA technique [32,33], we demonstrated that the concentrations of anti-retinal antibodies increased in the infected eyes during the early post-infection period. The data correlated with the degree of infectious tissue destruction at the clinical level (data not presented).…”

“…The level of antibodies against the retinal S-antigen and peptide 35 were similarly quantified using the enzymelinked immunosorbent assay (ELISA) technique [32,33]. and since there is a sequence homology between peptide 35 from rabbit, rat and human, we checked the antibody response to both, the entire bovine S-Ag molecule and to peptide 35 in toxoplasmic rabbits.…”

“…By drawing on an experimental rabbit model of systemic and primary and secondary ocular toxoplasmosis [30], we wished to investigate the onset and development of the humoral response to the retinal S-antigen, using an established autoantibody ELISA technique [31][32][33]. A comparison between primary ocular and systemic toxoplasmosis will reveal whether any cross-reactivity exists between toxoplasmal and retinal antigens, and that between primary and secondary OT will disclose whether a correlation exists between the levels of anti-retinal antibodies and the degree of ocular tissue destruction.…”