Immunological Characteristics of Hatano High-and Low-Avoidance Rats

Ohta, Ryo; Kanazawa, Yukiko; Shindo, Tomoko; Furuya, Mami; Shirota, Mariko; Kojima, Kohichi

doi:10.1538/expanim.55.369

Cited by 4 publications

(6 citation statements)

References 22 publications

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“…Therefore, further study of blood pressure is needed in older LAA rats, since age-related CPN was observed in all of the LAA rats. While polyarteritis nodosa also involves vasculitis associated with immune complex deposition that can lead to renal failure, the occurrence of polyarteritis nodosa might also be related to the fact that antibody production is higher in LAA rats than in HAA rats 39 .…”

Section: Discussionmentioning

confidence: 99%

Stress-reactive rats (high-avoidance female rats) have a shorter lifespan than stress-nonreactive rats (low-avoidance female rats)

et al. 2016

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Although Hatano high-avoidance and low-avoidance rats (HAA and LAA, respectively) have been selectively bred for good versus poor avoidance learning, HAA rats are known to be more reactive to stress than LAA rats. In this study, HAA and LAA female rats were compared during reproductive aging by observing estrous cycles from 8 to 11 months of age. Furthermore, these rats were allowed to live out their natural lifespans, that is, until 24 months of age, in order to compare their survival and to clarify the relationship between reproductive aging and tumor development. At eight months of age, 2 of 35 HAA rats and 20 of 35 LAA rats had abnormal estrous cycles. The median lifespan of the HAA rats (673 days) was shorter than that of the LAA rats (733 days). The incidence of pituitary neoplasia was higher in the HAA rats than in the LAA rats. These results suggest that HAA female rats (i.e., stress-reactive rats) have a shorter lifespan than LAA female rats (i.e., stress-nonreactive rats) and develop pituitary neoplasia, which was one of the causal factors in their accelerated mortality. However, the onset of an age-matched abnormal cycle did not correspond with their lifespan.

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Section: Discussionmentioning

confidence: 99%

Stress-reactive rats (high-avoidance female rats) have a shorter lifespan than stress-nonreactive rats (low-avoidance female rats)

et al. 2016

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“…Because there are close interconnections among nervous, endocrine, and immune systems, HAA and LAA lines may have differences in immunological activity. Therefore, the plasma levels of IgG, IgM, complement 3 (C3), classical pathway hemolytic complement (CH50), and beta-2-microglobulin were compared between males of the two lines at 5 and 24 weeks of age [28]. The plasma levels of IgG and CH50 were lower in the LAA line than in the HAA line at 5 weeks of age, whereas the differences disappeared at 24 weeks of age.…”

Section: Immunological Differences In Hatano Ratsmentioning

confidence: 99%

“…There were no differences between the two strains in the plasma levels of IgM, C3, and beta-2-microglobulin. Antibody production to sheep red blood cells (SRBC) and mitogen-induced lymphocyte proliferation were compared between 12-week-old males of the two lines [28]. Antibody responses in the PFC assay, plasma anti-SRBC-IgM levels, and spleen weights were higher in LAA lines than in HAA lines.…”

Section: Immunological Differences In Hatano Ratsmentioning

confidence: 99%

Hatano rats selectively bred for high- and low-avoidance learning: an overview

Ohta

Kojima

2019

Exp. Anim.

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This review compiles the results of a series of studies on Hatano high- and low-avoidance animals (HAA and LAA, respectively) established at the Hatano Research Institute, Food and Drug Safety Center, Japan. The HAA and LAA lines were selected and bred from Sprague Dawley rats for high and low avoidance learning, respectively, in a shuttlebox task since 1985. Although Hatano rats were selected only based on their behavioral traits in the active avoidance task, strain differences between the HAA and LAA lines were also observed in their stress responses and reproductive functions. However, the most noticeable finding of Hatano rats is a matched result in both active and passive avoidance tasks. The HAA and LAA lines are useful for next-generation toxicological studies, because the hereditary characters of behaviors or endocrine functions are well controlled.

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“…The animals were anesthetized with sodium pentobarbital, and blood was collected from the posterior vena cava, prior to sacrifice. The spleen was weighed, and the spleen cells were subjected to a plaque-formation cell (PFC) assay as previously described [26]. The blood's lymphocyte count was analyzed by an automated hematology analyzer (CellDyn3500, Abbott Laboratories), and then the serum was separated and stored at -80°C until determination of anti-SRBC-IgM [26].…”

Section: Immune Response To Sheep Red Blood Cells (Srbc)mentioning

confidence: 99%

“…Using these two strains, we have developed new methods for the risk assessment of the toxicological effects of substances on the behavior of the next-generation [28,30]. Although the Hatano rats are separated by their avoidance performance as mentioned above, characteristic differences between the strains are not only observed in their behaviors but also in their reproductive function [2,35,36,38], immunological reactions [26], and stress responses [1,3,31]. For example, compared to HAA rats, LAA rats show lower running-wheel activity, later sexual maturation, 5-day estrous cycling, lower sperm motility, more pronounced immunological reactions, and are generally less reactive to stress.…”

Section: Introductionmentioning

confidence: 99%

Effects of Transmaternal Exposure to Genistein in Hatano High- and Low-Avoidance Rats

et al. 2009

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Abstract:Hatano high-and low-avoidance (HAA and LAA) rats are separated by breeding from Sprague-Dawley rats by high versus low rates of avoidance responses in a shuttle-box task. In addition, compared to HAA rats, LAA rats show lower running-wheel activity, later sexual maturation, 5-day estrous cycling, lower sperm motility, more pronounced immunological reactions, and are generally less reactive to stress. The present study was designed to compare the effects of transmaternal exposure to genistein on these characteristics between HAA and LAA rats. To this aim, litters from both strains were fostered onto Sprague-Dawley rats receiving genistein by gavage with 5 mg/animal/day from day 17 of pregnancy through day 21 of lactation. Inhibited growth after weaning and reduced uterine weight at weaning were observed in the LAA offspring reared by genistein-treated dams. IgM antibody production in response to sheep red blood cells was significantly decreased in the HAA offspring reared by genistein-treated dams. During restraint stress, the plasma concentration of corticosterone was significantly lower in the LAA offspring reared by genistein-treated dams. Strain-related differences were detected in shuttle-box avoidance performance, running-wheel activity, estrous cycling, and sperm motility. The results demonstrate that transmaternal exposure to genistein potentially affects the immunological and stress responses as well as the postweaning growth of the offspring. It suggests that a comparative study using Hatano rats would be useful for studying the influence of endocrine active chemicals on the whole body systems.

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Immunological Characteristics of Hatano High-and Low-Avoidance Rats

Abstract: Abstract:Hatano high-and low-avoidance (HAA and LAA)

Cited by 4 publications

References 22 publications

Stress-reactive rats (high-avoidance female rats) have a shorter lifespan than stress-nonreactive rats (low-avoidance female rats)

Stress-reactive rats (high-avoidance female rats) have a shorter lifespan than stress-nonreactive rats (low-avoidance female rats)

Hatano rats selectively bred for high- and low-avoidance learning: an overview

Effects of Transmaternal Exposure to Genistein in Hatano High- and Low-Avoidance Rats

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