Immunolocalization of vascular endothelial growth factor, its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bitch uterus during the sexual cycle

Sağsöz, Hakan; Lïman, Narin; Küçükaslan, İbrahim; Saruhan, Berna Güney

doi:10.1016/j.anireprosci.2013.05.014

Cited by 9 publications

(13 citation statements)

References 49 publications

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“…) and muscle cells (Walsh and Grant ; Sağsöz et al . ). The observations made in the present study and information provided in the literature reports prompted us to ask whether the function of VEGF, flt1/fms and flk1/KDR in the non‐endothelial cells of the quail lung is to regulate cell survival or to prevent apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…The sections were stained using the method described in our previous work (Sağsöz and Saruhan ; Sağsöz et al . ). Following rehydration, endogenous peroxidase activity was blocked by incubating the slides in 3% H 2 O 2 in methanol for 15 min at room temperature and by washing them twice in PBS.…”

Section: Methodsmentioning

confidence: 97%

“…The evaluation of the immunohistochemical staining of the posthatching groups was made with the Quick score (total score) method described in our previous work (Sağsöz and Saruhan ; Sağsöz et al . ). The score for the proportion of cells stained (proportional score) and the score for the intensity of staining (intensity score) were added up to calculate the total score, which ranged from 0 to 8.…”

Section: Methodsmentioning

confidence: 97%

“…; Sağsöz and Saruhan ; Sağsöz et al . ) and suggest that while it induces apoptosis in endothelial cells (Yue et al . ; Sağsöz et al .…”

Section: Introductionmentioning

confidence: 99%

“…; Sağsöz et al . ), it suppresses postnatal vasculogenesis by inhibiting differentiation signals in early stage endothelial progenitor cells (EPCs) (Tian et al . ) and tumour growth (Liang et al .…”

Section: Introductionmentioning

confidence: 99%

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Physiological roles of the angiogenic factors during posthatching development period and adults in the quail lung

2015

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Sa gs€ oz H., Liman N. and Alan E. 2016. Physiological roles of the angiogenic factors during posthatching development period and adults in the quail lung. -Acta Zoologica (Stockholm) 97: 376-390.The bronchus and vasculature form an intrinsic functional component of the avian lung, and its growth must be tightly regulated and coordinated by lung epithelial and endothelial development. Vascular endothelial growth inhibitor (VEGI), vascular endothelial growth factor (VEGF) and its receptors (flk1/ KDR, flt1/fms, flt4) are required for epithelial and endothelial cell survival and apoptosis. Especially, VEGF and its receptors are critical for the development of the lung and serve as a maintenance factor during adult life. To determine the function of VEGI, VEGF and its receptors in the posthatching lung development, we revealed its expression and localization using by immunohistochemical procedure. VEGI, VEGF and its receptors were observed in the structural components of the bronchi, atria and air capillaries, as well as in the pulmonary blood vessels throughout the posthatching development period. On the other hand, immunostaining for VEGI, VEGF and its receptors was faintly detected in the glands of the secondary bronchi. Furthermore, it was determined that the secondary bronchial and atrial muscles did not display VEGF immunoreactions. Our results showed that VEGF and its receptors (flt1/fms, flk1/KDR and flt4) and VEGI were expressed at varying intensity by different cell groups. Therefore, they are also required for the development of the lung component during posthatching period. r; correlation coefficient; PhD; posthatching development periods; A, adult. 1 Correlation is significant at the 0.05 level. 2 Correlation is significant at the 0.01 level,

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

“…; Sağsöz and Saruhan ; Sağsöz et al . ) and suggest that while it induces apoptosis in endothelial cells (Yue et al . ; Sağsöz et al .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiological roles of the angiogenic factors during posthatching development period and adults in the quail lung

2015

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The expression of epidermal growth factor receptors and their ligands (epidermal growth factor, neuregulin, amphiregulin) in the bitch uterus during the estrus cycle

Sağsöz

Lïman

Saruhan

et al. 2014

Animal Reproduction Science

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Topical dimethyl sulfoxide inhibits corneal neovascularization and stimulates corneal repair in rabbits following acid burn

Altan¹,

Sağsöz

Oğurtan

2017

Biotechnic & Histochemistry

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Neovascularization of the cornea is characterized by the growth of blood vessels caused by imbalances between angiogenic and anti-angiogenic factors. We investigated whether the expression of Vascular endothelial growth factor (VEGF), Vascular endothelial growth factor receptor (VEGF), Vascular endothelial growth inhibitor (VEGI) receptors, as well as topical drug treatments, participate in regulating corneal neovascularization after corneal damage and remodeling. We used 72 mature male New Zealand rabbits. Corneal burns were induced by hydrofluoric acid under general anesthesia. The rabbits then were treated with indomethacin or dimethyl sulfoxide (DMSO). The animals were euthanized on days 2, 7 and 14 after injury. Each cornea was fixed with 10% neutral formalin. On days 2, 7 and 14, VEGF, flk1/KDR and flt1/fms were strongly expressed in the epithelial, stromal and inflammatory cells, but not in the corneal endothelial cells. On day 7, newly formed blood vessels were observed growing toward the center of the cornea. In the control, indomethacin treated, DMSO-treated, and indomethacin + DMSO-treated animals, VEGI, VEGF, and the receptors, flk1/KDR, flt1/fms and flt4, were expressed at different densities in the neovascular regions. This was particularly evident in the indomethacin- and indomethacin + DMSO-treated groups on days 7 and 14, compared to day 2. Treatment with VEGF and DMSO stimulated repair of corneal damage. We suggest that VEGI in the endothelial cells of neovascularized cornea may act as a signaling protein that promotes balance between cell proliferation and apoptosis. Topical administration of DMSO inhibited corneal neovascularization more effectively than indomethacin.

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Immunolocalization of vascular endothelial growth factor, its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bitch uterus during the sexual cycle

Cited by 9 publications

References 49 publications

Physiological roles of the angiogenic factors during posthatching development period and adults in the quail lung

Physiological roles of the angiogenic factors during posthatching development period and adults in the quail lung

The expression of epidermal growth factor receptors and their ligands (epidermal growth factor, neuregulin, amphiregulin) in the bitch uterus during the estrus cycle

Topical dimethyl sulfoxide inhibits corneal neovascularization and stimulates corneal repair in rabbits following acid burn

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