Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis

Gendek-Kubiak, Hanna; Gendek, Ewa G.

doi:10.1016/j.jneuroim.2003.12.023

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…However, the complement involvement in PM has not been excluded. The similarity of the pathological processes in PM and DM have been indirectly confirmed in our work [6] in which we showed a decreased expression of CD59 (a factor protecting cells from the complement induced lysis) in muscle fibers located in the regions affected by the diseases.…”

Section: Introductionsupporting

confidence: 74%

Can tissue transglutaminase be a marker of idiopathic inflammatory myopathies?

2005

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Section: Introductionsupporting

confidence: 74%

Can tissue transglutaminase be a marker of idiopathic inflammatory myopathies?

2005

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“…The complement regulatory protein CD59 is a glycosylphosphatidylinositol-anchored membrane protein which inhibits the formation of membrane attack complex (MAC) and protects the cells from MAC-induced lysis (Turnberg & Botto 2003). In comparison with control muscle, atrophied muscle fibers of inflammatory myopathic patients do not contain CD59 whereas regenerating muscle cells (myoblasts and myotubes) from the same patients have a strong immunostaining with CD59 antibody (Gendek-Kubiak & Gendek 2004). From the facts that (1) 2 M and CD59 are commonly highly expressed in myoblasts and regenerating fibers, (2) muscle regeneration induced by muscle injury and hypertrophy induced by growth stimuli share common processes of muscle growth and (3) many transcripts modulated at 24 h after DHT treatment in the current study were related to cell proliferation (mitosis), the elevated expression levels of B2 m and Cd59a 24 h after DHT injection might reflect myoblast proliferation.…”

Section: Immunitymentioning

confidence: 99%

Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression

Yoshioka

Boivin²,

Ye³

et al. 2006

Journal of Molecular Endocrinology

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In order to characterize the action of androgen in skeletal muscle, we have investigated the effects of castration (GDX) and dihydrotestosterone (DHT) on global gene expression in mice. The serial analysis of gene expression method was performed in the muscle of male mice in six experimental groups: intact, GDX and GDX+DHT injection 1, 3, 6 or 24 h before they were killed. A total of 780 822 sequenced tags quantified the expression level of 80 142 tag species. Thirteen and seventy-nine transcripts were differentially expressed in GDX and DHT respectively (P,0·05), including eight partially characterized and 21 potential novel transcripts. The induced transcripts within 3 h after DHT injection were involved in the following functions: transcription, protein synthesis, modification and degradation, muscle contraction and relaxation, cell signaling, polyamine biosynthesis, cell cycle progression and arrest, angiogenesis, energy metabolism and immunity. However, the inductions of transcripts related to cell cycle arrest and angiogenesis were no longer significant 24 h after DHT injection. The current study might suggest that DHT promotes protein synthesis, cell signaling, cell proliferation and ATP production, as well as muscle contraction and relaxation at the transcriptional level in skeletal muscle in vivo.

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“…23 In addition, conditions characterized by muscle damage, such as Duchenne muscular dystrophy, polymyositis, dermatomyositis, amyotrophic lateral sclerosis, and sepsis, are associated with increased muscle expression of CD59. 13,[24][25][26][27] The findings demonstrate that muscle cell damage may be susceptible to complement attack and may require protection by complement regulatory proteins in the pathologic conditions of the muscles.…”

Section: Discussionmentioning

confidence: 99%

CD59 Expression in Skeletal Muscles and Its Role in Myasthenia Gravis

Iwasa

Furukawa

Yoshikawa

et al. 2023

Neurol Neuroimmunol Neuroinflamm

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Background and ObjectivesComplement regulatory proteins at the neuromuscular junction (NMJ) could offer protection against complement-mediated damage in myasthenia gravis (MG). However, there is limited information on their expression at the human NMJ. Thus, this study aimed at investigating the expression of the cluster of differentiation 59 (CD59) at the NMJ of human muscle specimens and demonstrating the overexpression ofCD59mRNA and protein in the muscles of patients with MG.MethodsIn this observational study, muscle specimens from 16 patients with MG (9 and 7 patients with and without thymoma, respectively) and 6 nonmyopathy control patients were examined. Immunohistochemical stains, Western blot analysis, and quantitative real-time reverse transcription PCR were used to evaluate the CD59 expression.ResultsA strong localized expression of CD59 was observed at the NMJ in both patients with and without MG. Moreover, the CD59/glyceraldehyde-3-phosphate dehydrogenase protein ratio in patients with MG was significantly higher than that in the nonmyopathy controls (MG; n = 16, median 0.16, interquartile range (IQR) 0.08–0.26 and nonmyopathy controls; n = 6, median 0.03, IQR 0.02–0.11,p= 0.01). The proportion ofCD59mRNA expression relative toAChRmRNA expression (ΔCtCD59/AChR) was associated with the quantitative MG score, MG activities of daily living score, and MG of Foundation of America Clinical Classification (r= 0.663,p= 0.01;r= 0.638,p= 0.014; andr= 0.715,p= 0.003, respectively).DiscussionCD59, which acts as a complement regulator, may protect the NMJ from complement attack. Our findings could provide a basis for further research that investigates the underlying pathogenesis in MG and the immunomodulating interactions of the muscle cells.

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Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis

Cited by 6 publications

References 29 publications

Can tissue transglutaminase be a marker of idiopathic inflammatory myopathies?

Can tissue transglutaminase be a marker of idiopathic inflammatory myopathies?

Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression

CD59 Expression in Skeletal Muscles and Its Role in Myasthenia Gravis

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