Immunolocalization of prealbumin as a marker for carcinoid tumours

Seventeen cases of primary and one case of metastatic breast cancer which expressed greater than 900 fmol oestrogen receptor sites per mg soluble protein were examined. All these patients were post-menopausal at the time of their presentation. These were a heterogeneous group of well-differentiated cellular breast carcinomas, comprising cases of invasive duct carcinoma with extensive tubular differentiation or with focal argyrophilia, tubulolobular carcinoma, lobular carcinoma of mixed type containing abundant intracytoplasmic lumina, papillary carcinoma and type B colloid carcinoma. There was very little tumour necrosis. Nodal Keywords: cancer, breast, histology, oestrogen receptors metastasis, tumour size and host inflammatory response did not appear to show any relationship with oestrogen receptor status. The patients, apart from two who died from other causes, remain alive (Fisher's exact test, P

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“…Human pre-albumin, as demonstrated by Miller et al (1984), is a good marker for tumour cells displaying argyrophilia.…”

Section: Discussionmentioning

confidence: 99%

Breast cancers with extremely high oestrogen receptor protein status

1990

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“…23 Moreover, these tumours are often associated with symptoms related to excessive or inappropriate release of hormones.24 Although the morphology of most types of carcinoid tumours is distinctive and the diagnosis is usually strongly suspected before special stains are used, it can be variable. Some tumours, especially the less well differentiated or mucinous types, can present a diagnostic problem,56 and it is in these cases that further investigations are needed.…”

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confidence: 99%

Comparison of immunological detection of 5-hydroxytryptamine by monoclonal antibodies with standard silver stains as an aid to diagnosing carcinoid tumours.

Shaw

1988

J Clin Pathol

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Neuroendocrine tumours associated with the gut were described in the early 20th century, although their true nature was not recognised. In 1907 Oberndorfer labelled them "karzinoide tumoren",' a name which, in its English translation as carcinoid tumours, has been in widespread use ever since.Despite the fact that many carcinoid tumours appear to infiltrate diffusely surrounding tissues they often behave much less aggressively than adenocarcinomas and as a result have a much better prognosis.23 Moreover, these tumours are often associated with symptoms related to excessive or inappropriate release of hormones.24 Although the morphology of most types of carcinoid tumours is distinctive and the diagnosis is usually strongly suspected before special stains are used, it can be variable. Some tumours, especially the less well differentiated or mucinous types, can present a diagnostic problem,56 and it is in these cases that further investigations are needed.7Until recently the stains used routinely for confirmation of the diagnosis of carcinoid tumour were of the argyrophil, argentaffin, or diazo types. The success of argentaffin and diazo, however, depends on the presence of large amounts of 5-hydroxytryptamine Accepted for publication 6 October 1987 (5HT) within the tumour cells, and the argyrophil methods are not entirely specific.&' Over the past few years immunocytochemical techniques have become more widespread in routine laboratories, and it has been suggested that immunocytochemital localisation of 5HT "provides a simpler, more reliable procedure for the diagnosis of carcinoid tumours".9This study was undertaken to investigate that claim in a routine laboratory setting, paying special attention to subgroups ofcarcinoid tumours that may cause diagnostic difficulty and including in the series other neuroendocrine and non-neuroendocrine tumours. Material and methodsA search was made of the files of the histopathology departments of the Leicester Royal Infirmary and Leicester General Hospital. All specimens diagnosed as carcinoid tumours from April 1981 until January 1986 (the period for which computer records were available) were included in the series. Further searches were made to identify other neuroendocrine tumours. Where adequate tissue was available one or two blocks from each specimen were recut and sections were stained as detailed below. All tissues had undergone fixation for 12 to 36 hours in unbuffered formalin before routine processing and paraffin embedding.

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Immunolocalization of prealbumin: Distribution in normal human tissue

Liddle

Reid

Kennedy

et al. 1985

The Journal of Pathology

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Using an indirect immunoperoxidase staining technique we have demonstrated the plasma protein, prealbumin, in the cells of the pancreatic islets of Langerhans. Serial sections stained with antisera to glucagon, insulin and somatostatin revealed that the distribution of the prealbumin stained cells matched that of the glucagon A-cells. Absorption and cross-absorption experiments with prealbumin and glucagon showed that the staining was specific. We also found positive staining for prealbumin in single cells in the mucosa of the gastrointestinal tract, mainly stomach and colon, and kidney tubules.

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Immunolocalization of prealbumin as a marker for carcinoid tumours

Cited by 14 publications

References 14 publications

Breast cancers with extremely high oestrogen receptor protein status

Breast cancers with extremely high oestrogen receptor protein status

Comparison of immunological detection of 5-hydroxytryptamine by monoclonal antibodies with standard silver stains as an aid to diagnosing carcinoid tumours.

Immunolocalization of prealbumin: Distribution in normal human tissue

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