Immunolocalization of matrix metalloproteinases-2 and -9 during apical periodontitis development

Corotti, Mauro V.; Zambuzzi, Willian Fernando; Paiva, Katiúcia Batista Silva; Menezes, Renato; Pinto, Lidiane C.; Lara, Vanessa Soares; Granjeiro, José Mauro

doi:10.1016/j.archoralbio.2009.04.013

Cited by 74 publications

(75 citation statements)

References 36 publications

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“…It is essential for initiating the osteoclastic resorption process by removing the collagenous layer from the bone demineralization (14). MMP2 and MMP9 are known to specifically cleave Type IV collagen, which is a major structural component of the basal membrane (15).…”

Section: Introductionmentioning

confidence: 99%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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The aim of this study was to evaluate the expression of MMP2 and MMP9 during apical periodontitis (AP) progression in TLR2 (TLR2 KO) and in MyD88 (MyD88 KO) knockout mice compared to wild type (WT) mice. AP was induced in mandibular first molars of TLR2 KO (n= 18), MyD88 KO (n= 18), and WT mice (n= 18). After 7, 21, and 42 days, the animals were euthanized and the jaws were dissected and subjected to histotechnical processing. Subsequent sections were stained by immunohistochemistry and evaluated for detection of MMP2 and MMP9. Statistical analysis of the semi-quantitative analysis of immunohistochemistry was performed using chi-square test (α = 0.05). In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression. MMP2 and MMP9 are Associated w i t h A p i c a l P e r i o d o n t i t i s P r o g r e s s i o n a n d M i g h t b e Modulated by TLR2 and MyD88

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Section: Introductionmentioning

confidence: 99%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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“…Además, las MMPs no sólo son capaces de degradar la MEC periodontal, sino que mediante proteólisis limitada pueden modificar la actividad biológica de mediadores inflamatorios, actuando como moduladores de los procesos inmunoinflamatorios (8) . Los resultados obtenidos en este estudio respecto a la presencia de MMP-2 en LPAs, concuerdan con estudios anteriores (14,22) , donde se determinó la expresión de MMP-2 en plasmocitos, linfocitos y células endoteliales, tanto en GPA como QRI.…”

Section: Discussionunclassified

Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomática

Burgos

Piwonka

Alfaro

et al. 2011

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomáticaImmunodetection of matrix metalloproteinases (MMPs)-2, -9, -13 and -14 in periapical lesions associated with asymptomatic apical periodontitis RESUMENLa periodontitis apical asintomática (PAa) es una patología infecciosa caracterizada por destrucción ósea perirradicular asociada a un proceso inflamatorio crónico y producción de mediadores inflamatorios, entre los cuales se encuentran las metaloproteinasas de matriz extracelular (MMPs).Entre éstas, las MMPs-13, -14, -2 y -9, son producidas por el tejido óseo y degradan sinérgicamente el colágeno tipo I, principal componente de los tejidos periodontales, y gelatina, producto de la degradación y desnaturación del colágeno. El objetivo de este estudio fue determinar el patrón de expresión de las MMPs-2, -9, -13 y -14 en granulomas periapicales (GPAs), quistes radiculares inflamatorios (QRIs) y ligamento periodontal sano (LS). Materiales y Métodos:Se seleccionaron 12 pacientes con diagnóstico clínico de PAa e indicación de exodoncia a partir de los cuales se obtuvieron biopsias de lesiones periapicales (LPAs). Como controles, se seleccionaron 7 individuos con indicación de exodoncia de premolares por ortodoncia, obteniéndose biopsias de LS. Se efectuó el diagnóstico anátomo-patológico de los especímenes y se caracterizó la expresión de las MMPs en estudio mediante inmunohistoquímica. Resultados: Las MMPs en estudio sólo se detectaron en GPAs y QRIs, y se inmunolocalizaron principalmente en el infiltrado inflamatorio de éstos. Adicionalmente, la MMP-2 se identificó en fibroblastos del tejido conectivo. Conclusiones: MMPs-2, -9, -13 y -14 se expresan predominantemente en el infiltrado inflamatorio de las LPAs y no en LS, y por tanto se sugiere la participación de estos mediadores en la patogénesis de la PAa. Rev. Clin. Periodoncia Implantol. Rehabil. Oral Vol. 4(1); 17-21, 2011.Palabras clave: Periodontitis apical asintomática, lesiones periapicales, metaloproteinasas de matriz extracelular. ABSTRACTAsymptomatic apical periodontitis (aAP) is an infectious disease characterized by perirradicular bone destruction associated with chronic inflammation and release of inflammatory mediators, such as matrix metalloproteinases (MMPs). MMPs-13, -14 and -2, -9 are bone-expressed enzymes that can synergistically degrade collagen I, the main component of periodontal extracellular matrix, and gelatin, the product of degradation and denaturation of collagen. The aim of this study was to characterize the expression pattern of MMPs-2, -9, -13, and -14 in periapical granulomas (PGs), radicular cysts (RCs) and healthy periodontal ligament (PDL). Materials and Methods: Individuals with clinical diagnosis of aAP and indication of extraction were selected (N=12), and biopsies of periapical lesions (PLs) were obtained. For controls, 7 subjects with indication of premolar extraction for orthodontic reasons were selected, and PDL biopsies wer...

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“…2,31,33 Particularly noteworthy is that expressions of TGF-β1 and MMP-9 may depend on the secretion of other proteins, such as cytokines, growth factors, transcriptional factors, other matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases (TIMPs). 2,3,4,5,6,31,32,33,34,35,36 Experiments using MMP-9 knockout mice 14 evidenced that these animals exhibited larger periapical lesions with greater inflammation, indicating a central participation of MMP-9 in the development of periapical lesions. The presence of TGF-β1 and MMP-9 in periapical lesions denotes their important role in the maintenance, development and exacerbation of chronic processes, 5,14,34,35 although further studies are needed to evaluate the possible molecular mechanisms involved in the progression of these lesions.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

et al. 2017

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The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

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Immunolocalization of matrix metalloproteinases-2 and -9 during apical periodontitis development

Abstract: The results observed in this study suggest that MMP-2 and MMP-9 play a critical role in the development of inflammatory periapical lesions, probably involved in the extracellular matrix (ECM) degradation during the initial phase of the lesion development.

Cited by 74 publications

References 36 publications

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomática

Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

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