Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland

Isobe, Naoki; Hosoda, Kenji; Yoshimura, Y.

doi:10.1111/j.1740-0929.2009.00652.x

Cited by 52 publications

(53 citation statements)

References 17 publications

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“…Immunohistochemical studies demonstrated the expression of LAP peptide in healthy (Isobe et al . 2009b) and E. coli ‐inoculated mammary epithelial cells (Petzl et al . ).…”

Section: Introductionmentioning

confidence: 99%

Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count

Isobe

Shibata

Kubota

et al. 2013

Animal Science Journal

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The present study was undertaken to examine whether potential levels of innate immune factors (lingual antimicrobial peptide (LAP), lactoferrin (LF) and lactoperoxidase (LPO)) in colostrum are associated with subsequent milk somatic cell count (SCC) in dairy cows. Quarter milk samples were collected daily for 1 week postpartum to measure LAP and LF concentrations and LPO activity. SCC in milk was determined weekly for 2 months postpartum and its correlations to concentrations of LAP and LF and LPO activity were examined. Only small variations of all immune factors were found among four udders in each individual cow, whereas there were great differences in these factors among cows. Negative correlation was detected only between LPO activity and mean and maximum SCC, whereas its relationship was not significant. LAP and LF concentrations were significantly correlated positively to mean, maximum and minimum SCC. These results suggest that the great difference in innate immune factors among animals and high LAP and LF concentrations in colostrum may be associated with subsequent high incidence of SCC increase.

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“…Immunohistochemical studies demonstrated the expression of LAP peptide in healthy (Isobe et al . 2009b) and E. coli ‐inoculated mammary epithelial cells (Petzl et al . ).…”

Section: Introductionmentioning

confidence: 99%

Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count

Isobe

Shibata

Kubota

et al. 2013

Animal Science Journal

Self Cite

View full text Add to dashboard Cite

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“…Therefore, we developed LAP antibody using rabbits for immunohistochemical studies. The results demonstrated the expression of LAP peptide in the epithelial cells of alveoli and milk ducts of healthy mammary glands (Isobe et al 2009a; Fig. 1).…”

Section: B-defensinmentioning

confidence: 74%

“…The results demonstrated the expression of LAP peptide in the epithelial cells of alveoli and milk ducts of healthy mammary glands (Isobe et al . ; Fig. ).…”

Section: β‐Defensinmentioning

confidence: 93%

Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Isobe

2017

Animal Science Journal

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Mastitis, a symptom of inflammation in mammary tissue by infection with various kinds of bacteria, causes huge economic losses in the milk industry. One of the popular methods for treatment of mastitis is antibiotics, although this prohibits milk shipping and sometimes causes resistant microbes. Therefore, a new strategy to treat mastitis without antibiotics is eagerly required around the world. Antimicrobial factors belong to innate immunity and can start their function extremely early after bacterial stimulation. These factors have antimicrobial activity for a broad spectrum of bacteria. Elucidation of causal mechanisms and functions of antimicrobial factors in the mammary gland is thought to result in suitable methods for prevention and treatment of mastitis. Therefore, this review introduces traits of some antimicrobial factors and the mechanisms for expressing, producing and secreting them in the mammary gland. For antimicrobial factors, lingual antimicrobial peptide (LAP), S100A7, cathelicidin and lactoferrin are controlled in different sites and different time courses, suggesting that antimicrobial factors play different roles for local defense against bacterial infection in the mammary gland. These findings will contribute to the development of prevention and treatment methods for mastitis.

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“…Concentrations of S100A7 and S100A8 were determined by enzyme immunoassay (EIA) as described previously (Isobe, Hosoda, & Yoshimura, 2009;Zhang, Lai, Yoshimura, & Isobe, 2014). Antibodies against synthetic S100A7 and S100A8 peptides consisting of 14 and 11 amino acids (CFEKQDKNKDRKID and CLEAHEIHKE), respectively, were raised in rabbits.…”

Section: Measurement Of S100a7 and S100a8mentioning

confidence: 99%

Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy

Elgawish

Ogata

Hidaka

et al. 2018

Reprod Domestic Animals

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This study was carried out to examine the changes in plasma concentrations of the Ca-binding antimicrobial proteins S100A7 and S100A8 during pregnancy in dairy cows. Holstein Friesian cows (n = 19) were inseminated with Holstein Friesian semen. Blood was collected at days 30, 60, 90, 120, 150, 180, 210, 240 and 270 after insemination. Plasma was used for measuring the concentrations of S100A7 and S100A8. Both S100A7 and S100A8 concentrations showed similar patterns during gestation; they increased during the midgestation, between days 90 and 180, and then declined before calving. The findings indicated that plasma concentrations of S100A7 and S100A8 did not change significantly during pregnancy in cows. Further studies are required to determine the roles of S100A7 and S100A8 in physiological function during pregnancy in dairy cows.

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Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland

Cited by 52 publications

References 17 publications

Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count

Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count

Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy

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