Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count

Abstract

The present study was undertaken to examine whether potential levels of innate immune factors (lingual antimicrobial peptide (LAP), lactoferrin (LF) and lactoperoxidase (LPO)) in colostrum are associated with subsequent milk somatic cell count (SCC) in dairy cows. Quarter milk samples were collected daily for 1 week postpartum to measure LAP and LF concentrations and LPO activity. SCC in milk was determined weekly for 2 months postpartum and its correlations to concentrations of LAP and LF and LPO activity were examined. Only small variations of all immune factors were found among four udders in each individual cow, whereas there were great differences in these factors among cows. Negative correlation was detected only between LPO activity and mean and maximum SCC, whereas its relationship was not significant. LAP and LF concentrations were significantly correlated positively to mean, maximum and minimum SCC. These results suggest that the great difference in innate immune factors among animals and high LAP and LF concentrations in colostrum may be associated with subsequent high incidence of SCC increase.

Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet

“…Furthermore, the western blot results confirmed that the LAP protein concentration in the HCD group was higher than that in the LCD group and had a strong correlation with its corresponding relative copy numbers of mRNA. Additionally, the finding that the SCC and the LAP protein concentration both increased in the HCD group compared with those in the LCD group is consistent with Kawai et al [ 39 ] and Isobe et al [ 40 ], who found that the SCC is associated with the LAP protein concentration in the milk. Recently, Kawai et al [ 41 ] also found that LF concentration decreased with decrease in SCC after antibiotics treatment, but not LAP concentration and LPO activity, which differed depending on the severity of mastitis.…”

Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows

“…LF, and S100A7, and for IgA. The measurement methods for LAP, LF, and S100A7 were in accordance with Isobe, Morimoto, Nakamura, Yamasaki, and Yoshimura (2009b), Isobe, Shibata, Kubota, and Yoshimura (2013), and Zhang et al (2014), respectively. The IgA antibody concentrations were determined using the method of Matsukawa, Ueno, and Sugino (2018).…”

“…LF, and S100A7, and for IgA. The measurement methods for LAP, LF, and S100A7 were in accordance with Isobe, Morimoto, Nakamura, Yamasaki, and Yoshimura (2009b), Isobe, Shibata, Kubota, and Yoshimura (2013), and Zhang et al…”

Dynamics of lingual antimicrobial peptide, lactoferrin concentrations and lactoperoxidase activity in the milk of cows treated for clinical mastitis

“…The second aliquot of milk was defatted by centrifugation at 1700 × g for 30 min at 4°C and stored at −30°C until the measurement of LAP and LF concentrations and LPO activity (Isobe et al . ).…”