Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

Prihatno, Surya Agus; Adi, Yosua Kristian; Budipitojo, Teguh

doi:10.5455/javar.2020.g448

Cited by 11 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spermatogenesis occurs as a result of a complicated interaction between spermatogenic cells and testicular interstitial cells, which is facilitated by an appropriate environment. These include endocrine, paracrine, and autocrine systems [ 7 ]. The secretome contains basic fibroblast growth factor, a growth factor that promotes Sertoli cell proliferation in the testes and also stimulates Sertoli cells to make glutatonin.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, secretome has been studied as a potential therapeutic paradigm for central nervous system neurodegenerative diseases [ 6 ]. Secretome infusion appears to be able to modulate certain cytokines such as IL6 and IL10 during the testicular regeneration process [ 7 ]. It is not limited to soft organs; it has been demonstrated that secretome therapy promotes osteogenic differentiation in animals with faulty osteogenesis [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

Datrianto¹,

Budipitojo²,

Prihatno³

2021

J Adv Vet Anim Res

Self Cite

View full text Add to dashboard Cite

Objective: This study was determined to see the effects of secretome on cellular production of testosterone and androgen-binding protein (ABP) using immunohistochemistry in testicle dysfunction due to cisplatin. Materials and Methods: Forty-eight rats were divided into four groups: the healthy group, the group with testicular dysfunction, the secretome-treated group with 0.2 ml/kg body weight (BW), and the secretome-treated group with 0.3 ml/kg BW. The immunohistochemistry staining method is used to find out testosterone and ABP reactivity in tissue organs. Results: Very strong testosterone and ABP immunoreactivity were found in Leydig cells of normal testes. While in the Leydig cells of cisplatin-induced testicles, testosterone and ABP immunoreactivity were not observed. Testosterone and ABP were observed 1 week after the second secretome injection. The number of testosterone-immunoreactive cells in the low dose group from week 1 to 3 was 0, 19, and 32, respectively. From week 1 until week 4, the high dose group was 0, 29, 33, and 65, respectively. The number of ABP-immunoreactive cells from the first week until the third week in the low dose group was 0, 28, and 34, respectively. The high dose group from the first week until the fourth week was 0, 26, 58, and 83, respectively. The number of cells that produce testosterone and ABP increased from week 2 to 4. Conclusion: The administration of secretome could increase the number of immunoreactive cells toward testosterone and ABP in testicular dysfunction.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

Datrianto¹,

Budipitojo²,

Prihatno³

2021

J Adv Vet Anim Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Histone Post-Translational Modifications (PTM), as well as histone-writers and reader proteins are critical regulators of the gene expression programs in both the germline and the zygote. From C. elegans to the mouse, several PTMs in sperms and oocytes including mono and di-methylations of histone H3 at lysine 4 (H3K4me1/me2) and trimethylation of histone 3 at lysine 9 (H3K9me3), are retained at genomic regulatory elements (118), imprinted regions and miRNA clusters (119,120). Disruptions in the histone retention regions in sperms produced by environmental stimuli can transgenerationally persist up to the third generation (121)(122)(123).…”

Section: Epigenetic Inheritance Of Environmental Experiences: Can Bacteria Modify the Germline?mentioning

confidence: 99%

The Heritability of Behaviors Associated With the Host Gut Microbiota

2021

View full text Add to dashboard Cite

What defines whether the interaction between environment and organism creates a genetic memory able to be transferred to subsequent generations? Bacteria and the products of their metabolism are the most ubiquitous biotic environments to which every living organism is exposed. Both microbiota and host establish a framework where environmental and genetic factors are integrated to produce adaptive life traits, some of which can be inherited. Thus, the interplay between host and microbe is a powerful model to study how phenotypic plasticity is inherited. Communication between host and microbe can occur through diverse molecules such as small RNAs (sRNAs) and the RNA interference machinery, which have emerged as mediators and carriers of heritable environmentally induced responses. Notwithstanding, it is still unclear how the organism integrates sRNA signaling between different tissues to orchestrate a systemic bacterially induced response that can be inherited. Here we discuss current evidence of heritability produced by the intestinal microbiota from several species. Neurons and gut are the sensing systems involved in transmitting changes through transcriptional and post-transcriptional modifications to the gonads. Germ cells express inflammatory receptors, and their development and function are regulated by host and bacterial metabolites and sRNAs thus suggesting that the dynamic interplay between host and microbe underlies the host’s capacity to transmit heritable behaviors. We discuss how the host detects changes in the microbiota that can modulate germ cells genomic functions. We also explore the nature of the interactions that leave permanent or long-term memory in the host and propose mechanisms by which the microbiota can regulate the development and epigenetic reprogramming of germ cells, thus influencing the inheritance of the host. We highlight the vast contribution of the bacterivore nematode C. elegans and its commensal and pathogenic bacteria to the understanding on how behavioral adaptations can be inter and transgenerational inherited.

show abstract

“…Testicular tissues were collected one week after the first, second, third, and fourth injection of mesenchymal stem cells-conditioned medium. Testicular tissues were fixed in Bouin's solution for 24 hours, embedded in paraffin, sectioned to a thickness of 5 µm, and stained with the immunological method (Prihatno et al, 2020) to visualize the immunoreactivity of HSD3B1, HSD17B1, and P450scc.…”

Section: Sample Collectionmentioning

confidence: 99%

“…The slides were washed in Phosphate Buffer Saline (PBS) for five minutes, then repeated three times. Immunoreactivity was visualized with rabbit Diaminobenzidine (DAB) Detection IHC Kit (Fine Test, Wuhan Fine Biotech Co., Ltd., China) (Prihatno et al, 2020). After that, the slides were incubated overnight at 4 0 C with HSD3B1 polyclonal antibody (1: 200, MBS2530192, Mybiosource, USA), HSD17B1 polyclonal antibody (1:400, A10839, ABclonal, USA), and Cytochrome P450scc Enzyme polyclonal antibody (1:500, AB1244, MilliporeSigma, USA).…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Immunolocalization of Steroidogenic Enzymes (3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, and P450scc) in Rats with Testicular Dysfunction Treated with Mesenchymal Stem Cells-conditioned Medium

Khasanah¹,

Budipitojo²,

Fibrianto³

2021

WVJ

Self Cite

View full text Add to dashboard Cite

About 60-80 million couples in the world are suffering from infertility disease. Infertility is a major problem in patients coping with chemotherapy. The chemotherapy process can degenerate non-target organs, especially in testes. Infertility in male or testicular dysfunction is caused by the failure of proliferation and differentiation of the spermatogenic cells. Many studies reported that mesenchymal stem cells-conditioned medium promoted regenerative processes. The present study aimed to investigate the effect of mesenchymal stem cells-conditioned medium on the cisplatin-induced testicular dysfunction by examining the immunolocalization of steroidogenic enzymes, such as 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, and P450scc which are considered as markers of steroid production. All experimental animals were divided into three groups, namely the control group, mesenchymal stem cells-conditioned medium treated group with an injection dose of 0.2 ml/kg body weight (BW, P1), and mesenchymal stem cells-conditioned medium treated group with an injection dose of 0.5 ml/kg BW (P2). Cisplatin was injected into both treated groups to induce testicular dysfunction. The testicular tissues were processed by the paraffin method, then cut to a thickness of 5 µm, followed by immunohistochemical staining. The HSD3B1 immunoreactivities were found only in Leydig cells, and the intensity increased every week after the injection of mesenchymal stem cells-conditioned medium. The variety of weeks and groups was significantly different in the number of immunoreactive cells of HSD3B1. The results indicated a significant difference between one week after the first injection and the one week after the third and fourth injection. The findings showed a significant difference between the treated group with an injection dose of 0.2 ml/kg BW and the control group. The number of immunoreactive cells of HSD3B1 with an injection dose of 0.5 ml/kg BW was greater compared to the group that received an injection dose of 0.2 ml/kg BW. The intensity of HSD3B1 and HSD17B1 increased every week. The p450scc immunoreactive cells were only found in Leydig cells. The intensity of positive cells of p450scc in the treated group with an injection dose of 0.5 ml/kg BW was more intense, compared to the treated group with an injection dose of 0.2 ml/kg BW. The results of the current study showed that the injection of mesenchymal stem cells-conditioned medium can improve the regeneration of spermatogenic cells, and recover spermatogenesis proved by positive cells of HSD3B1, HSD17B1, and p450scc as markers of steroid production.

show abstract

Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

Cited by 11 publications

References 30 publications

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin

The Heritability of Behaviors Associated With the Host Gut Microbiota

Immunolocalization of Steroidogenic Enzymes (3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, and P450scc) in Rats with Testicular Dysfunction Treated with Mesenchymal Stem Cells-conditioned Medium

Contact Info

Product

Resources

About