Immunolocalization of Gas7 in the Subgranular Zone of Mice Hippocampus

Kumar, Udaya

doi:10.14712/23362936.2019.16

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…Studies have shown that it is involved in the growth of nerve axons in vitro. Increased expression of Gas7 can promote the mi- gration of neurons; inhibition of its expression can suppress the migration process of neurons [8][9][10]. It has been found that a high level of Gas7 expression protects neurons from damage caused by hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture Increases the Expression of Gas7 and NGF in the Prefrontal Cortex of Male Rats with Focal Cerebral Ischemia

Ma¹,

Dong²,

Y³

et al. 2023

J. Integr. Neurosci.

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Objective: To investigate the potential mechanisms underlying the migration of endogenous neural stem cells (eNSCs) to the frontal cortex to differentiate into neurons, and to monitor the effect of electroacupuncture (EA) regulation of focal cerebral ischemia (FCI) in rats on the expression of growth arrest-specific protein 7 (Gas7) and nerve growth factor (NGF) in the prefrontal cortex (PFC). Methods: Randomly, forty-eight male Sprague-Dawley rats were divided into four groups: Normal, Sham operation, Model, and EA. The right middle cerebral artery was embolized utilizing the thread-embolism technique. In the EA group, "Baihui" and "Zusanli" points were treated with electroacupuncture for 30 minutes, once a day, for 21 days. Nissl staining revealed the neuronal morphology of the PFC. Using immunohistochemistry and Western blot, the expression of Gas7 and NGF in the right PFC was observed. Results: Nissl staining showed clear PFC neurons with centered nuclei and distinct nucleoli in the Normal and Sham groups. In the Model group, the PFC nuclei were distinctively smaller. The neuronal morphology in the EA group resembled that of the Normal group. Results from Western blot and immunohistochemistry were comparable. The expression of Gas7 and NGF in the Sham surgery group did not differ significantly from the Normal group. However, the expression of Gas7 and NGF in the Model group was significantly lower than in the Normal group. The expression of Gas7 and NGF was significantly higher in the EA group than in the Model group. Conclusions: EA can increase the expressions of Gas7 and NGF in the ischemic prefrontal cortex, which may be one of the mechanisms by which EA promotes the differentiation of eNSCs into neurons in the injured area.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture Increases the Expression of Gas7 and NGF in the Prefrontal Cortex of Male Rats with Focal Cerebral Ischemia

Ma¹,

Dong²,

Y³

et al. 2023

J. Integr. Neurosci.

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“…Previous studies have demonstrated the independent expression of Disc1 and Gas7 protein in rodent brain [8, 12, 13]. But to-date, no studies have shown their co-expression in brain tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The procedure for immunohistochemistry was performed as per the protocol described in the previous publication [12]. In specific, mice were euthanized with sodium pentobarbital (100 mg/kg) and perfused with ice-cold phosphate-buffered saline (PBS), followed by 4% paraformaldehyde.…”

Section: Methodsmentioning

confidence: 99%

Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain

Kumar

2022

Brain Science Advances

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Objective: The aim of the present study was to check the potential interaction of two neurodevelopmental proteins, Disc1 and Gas7, in the adult mice brain. Methods: Twenty-four male Swiss albino mice were used for the study. The mice were 12 weeks old in the beginning of the experiment. Immunohistochemistry and co-immunofluorescence were performed on the coronal sections of mice brain and immunoblotting and co-immunoprecipitation were done on the whole brain lysate. Results: Data from immunohistochemistry and co-immunofluorescence indicate the occurrence and co-localization of Disc1 and Gas7 proteins in soma and projections of the brain cells. Immunostaining was observed in cerebral cortex, hypothalamus, midbrain, pons, medulla oblongata and CA3 of hippocampus of the brain. The data from Immunoblotting and co-immunoprecipitation validates the presence and interaction of Disc1 and Gas7 protein in whole brain lysate. Conclusion: Data indicates the potential interaction of Disc1 and Gas7 protein in adult brain. The study highlights the need for further research on Disc1–Gas7 protein interaction in brain development and neuro-disorders.

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“…It has been reported that growth arrest-specific (GAS) proteins play an important role in the regulation of multiple biological processes such as microfilament organization, neuronal differentiation, apoptosis, tyrosine kinase receptor activity, and control of the cell cycle progression [257][258][259][260][261][262]. GAS7, located on chromosome 17p13.1 [106], is expressed in early embryonic cells, testis, and neurons of several regions of the brain [263][264][265][266]. Furthermore, it has been demonstrated that GAS7 is expressed in the optic nerve and lamina cribrosa, which belongs to the connective tissue network via which the nerve fibers traverse to create the optic nerve, and it is predicted that the mentioned structure may constitute the main site for glaucomatous damage to the optic nerve.…”

Section: Gas7mentioning

confidence: 99%

The Genetic and Endoplasmic Reticulum-Mediated Molecular Mechanisms of Primary Open-Angle Glaucoma

Rozpędek‐Kamińska

Wojtczak

Szaflik

et al. 2020

IJMS

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Glaucoma is a heterogenous, chronic, progressive group of eye diseases, which results in irreversible loss of vision. There are several types of glaucoma, whereas the primary open-angle glaucoma (POAG) constitutes the most common type of glaucoma, accounting for three-quarters of all glaucoma cases. The pathological mechanisms leading to POAG pathogenesis are multifactorial and still poorly understood, but it is commonly known that significantly elevated intraocular pressure (IOP) plays a crucial role in POAG pathogenesis. Besides, genetic predisposition and aggregation of abrogated proteins within the endoplasmic reticulum (ER) lumen and subsequent activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK)-dependent unfolded protein response (UPR) signaling pathway may also constitute important factors for POAG pathogenesis at the molecular level. Glaucoma is commonly known as a ‘silent thief of sight’, as it remains asymptomatic until later stages, and thus its diagnosis is frequently delayed. Thereby, detailed knowledge about the glaucoma pathophysiology is necessary to develop both biochemical and genetic tests to improve its early diagnosis as well as develop a novel, ground-breaking treatment strategy, as currently used medical therapies against glaucoma are limited and may evoke numerous adverse side-effects in patients.

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Immunolocalization of Gas7 in the Subgranular Zone of Mice Hippocampus

Cited by 4 publications

References 19 publications

Electroacupuncture Increases the Expression of Gas7 and NGF in the Prefrontal Cortex of Male Rats with Focal Cerebral Ischemia

Electroacupuncture Increases the Expression of Gas7 and NGF in the Prefrontal Cortex of Male Rats with Focal Cerebral Ischemia

Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain

The Genetic and Endoplasmic Reticulum-Mediated Molecular Mechanisms of Primary Open-Angle Glaucoma

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