Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain

Abstract

Objective: The aim of the present study was to check the potential interaction of two neurodevelopmental proteins, Disc1 and Gas7, in the adult mice brain. Methods: Twenty-four male Swiss albino mice were used for the study. The mice were 12 weeks old in the beginning of the experiment. Immunohistochemistry and co-immunofluorescence were performed on the coronal sections of mice brain and immunoblotting and co-immunoprecipitation were done on the whole brain lysate. Results: Data from immunohistochemistry and co-immunofluorescence indicate the occurrence and co-localization of Disc1 and Gas7 proteins in soma and projections of the brain cells. Immunostaining was observed in cerebral cortex, hypothalamus, midbrain, pons, medulla oblongata and CA3 of hippocampus of the brain. The data from Immunoblotting and co-immunoprecipitation validates the presence and interaction of Disc1 and Gas7 protein in whole brain lysate. Conclusion: Data indicates the potential interaction of Disc1 and Gas7 protein in adult brain. The study highlights the need for further research on Disc1–Gas7 protein interaction in brain development and neuro-disorders.

HK3 stimulates immune cell infiltration to promote glioma deterioration

“…In brief, paraffin sections were incubated at 80 °C for 15 min, dewaxed in xylene, washed in gradient ethanol solutions, and rehydrated in double-distilled water. Then, the slides were pretreated by steaming them in sodium citrate buffer for 15 min at 95 °C for antigen retrieval [ 46 ]. After rinsing in PBS for 3 min, the slides were immunostained with primary antibodies against CD206 (1:100; Abclonal, cat.A8301), CD4 (1:100; Affinity, cat.…”