Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Knapczyk, Katarzyna; Duda, Małgorzata; Szafrańska, Bożena; Wolsza, Katarzyna; Panasiewicz, Grzegorz; Koziorowski, Marek; Słomczyńska, Maria

doi:10.1556/avet.56.2008.2.10

Cited by 12 publications

(5 citation statements)

References 40 publications

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“…developmental days E15–E19), mouse foetal lung tissues did not express detectable levels of ERα, which is consistent with the absence of ERα expression observed in adult lung tissues. Similar results were also obtained in an immunohistochemistry study of porcine embryo and foetus lung tissues (Knapczyk et al., 2008).…”

Section: Discussionsupporting

confidence: 85%

Expression of Oestrogen Receptors in Foetal Lung Tissue of Mice

Carvalho¹,

Gonçalves

2011

Anat Histol Embryol

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Oestrogens are responsible for the sexual dimorphism in adult mice lung tissue, and this difference is most notable at sexual maturity. Oestrogen receptor-alpha (ERα) and the oestrogen receptor-beta (ERβ) are the two receptors that mediate oestrogen action, but adult mice lung tissue only expresses ERβ, and it is probably through this receptor that oestrogens exert their action. The goal of our study was to detect the expression of ERα and ERβ in mouse foetal lung tissues and identify possible gender differences. The foetal lung tissue was collected between developmental days E15-E19, processed for histology and the expression of oestrogen receptors was detected by immunohistochemistry. Over the 5 days of lung development that were evaluated ERα was not expressed in the foetal lung tissues of neither male nor female mice. In contrast, ERβ was detected in both sexes, although the immunoreactivity differed for each developmental day whilst the staining intensity observed for ERβ also indicated differences between male and female lung tissues. The results demonstrate the existence of a gender difference in the foetal expression of ERβ in lungs of mice.

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Section: Discussionsupporting

confidence: 85%

Expression of Oestrogen Receptors in Foetal Lung Tissue of Mice

Carvalho¹,

Gonçalves

2011

Anat Histol Embryol

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“…24 The levels reported in studies using comparable levels of soy protein to our study, however, are sufficient to achieve significant interaction with the beta-type estrogen receptor, 24 which is expressed in the kidney throughout development. 25 The relationship of this pathway to renal injury is not clearly determined, but linkage to collagen synthesis that is an integral part for both glomerulosclerosis and interstitial nephritis has been described. 26 We could not, however, detect a difference in this specific parameter between HIS and LIS diets.…”

Section: Discussionmentioning

confidence: 99%

Dietary soy protein benefit in experimental kidney disease is preserved after isoflavone depletion of diet

Ogborn

Nitschmann²,

Bankovic-Calic³

et al. 2010

Exp Biol Med (Maywood)

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Soy diet ameliorates renal injury in the Han:SPRD-cy rat. The relative roles of protein, isoflavones and changes in polyunsaturated fatty acid (PUFA) status are not determined. We fed male Han:SPRD-cy heterozygotes casein (C), high isoflavone soy protein (HIS), alcohol-extracted low isoflavone soy protein (LIS) or mixed soy protein diet (MIS). LIS and MIS were associated with a small decrease in animal weight compared with HIS or C. Soy diets preserved normal renal function and reduced relative renal weight (10.9-14.6 g/kg, cf. 23.6, P < 0.001), scores for cystic change (0.168-0.239, cf. 0.386, P < 0.05), fibrosis (0.013-0.015, cf. 0.032, P < 0.05), tissue oxidized LDL content (0.012-0.021, cf. 0.048, P < 0.05), inflammation (8.5-12.9, cf. 31.2, P < 0.05) and epithelial cell proliferation (6.5-13.8, cf. 26.3, P < 0.05). In post hoc testing, LIS produced a greater reduction in relative renal weight, cystic change and epithelial proliferation, whereas HIS produced a significantly greater reduction in oxidized-LDL. Soy diets were associated with increased hepatic content of 18C PUFA (P < 0.001). LIS and HIS diets were associated with a small increase in body fat content (P < 0.001). Alcohol-extracted soy protein retains its major protective effects in this model with subtle differences attributable to isoflavones.

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“…2001) and during pregnancy (Knapczyk et al. 2008a) as well as in the developing porcine foetuses (Knapczyk et al. 2008b).…”

Section: Introductionmentioning

confidence: 99%

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

Knapczyk-Stwora

Durlej

Duda

et al. 2011

Reprod Domestic Animals

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The uterus is a well-known target of endocrine, paracrine and autocrine acting molecules among which steroid hormones are of special importance. The objective of our work was to localize oestrogen receptors (ERα and ERβ) mRNA and protein in the pig uterus throughout pregnancy (10, 18, 32, 50, 71, 90 days post coitum) using RT-PCR, Western-blot and immunohistochemistry. The present study is the first one to demonstrate the presence of ERs protein in the porcine uterus not only at the beginning but also at mid- and late pregnancy. In the pregnant swine, ERα was immunolocalized in the luminal epithelium (LE) and glandular epithelium (GE) and the myometrium of the uterus with differences in the intensity of staining at different stages of pregnancy studied. The LE and GE of pregnant swine stained for ERβ regardless of the day of pregnancy examined, whereas only a few cells within the myometrium showed a weak immunoreactivity. Western blot analysis confirmed the presence of ERα and ERβ proteins on all investigated days of gestation. The expression of ERα and ERβ mRNA was detected by RT-PCR in all examined samples corresponding to each of the consecutive stages of pregnancy. The obtained results show that ERα is more abundant in comparison to ERβ within the porcine pregnant uterus. The presence of ERα and ERβ in all compartments of the pig uterus during pregnancy may indicate direct action of oestrogens on proliferation and differentiation of these cells.

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Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Cited by 12 publications

References 40 publications

Expression of Oestrogen Receptors in Foetal Lung Tissue of Mice

Expression of Oestrogen Receptors in Foetal Lung Tissue of Mice

Dietary soy protein benefit in experimental kidney disease is preserved after isoflavone depletion of diet

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

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