Expression of Oestrogen Receptors in Foetal Lung Tissue of Mice

Abstract

Oestrogens are responsible for the sexual dimorphism in adult mice lung tissue, and this difference is most notable at sexual maturity. Oestrogen receptor-alpha (ERα) and the oestrogen receptor-beta (ERβ) are the two receptors that mediate oestrogen action, but adult mice lung tissue only expresses ERβ, and it is probably through this receptor that oestrogens exert their action. The goal of our study was to detect the expression of ERα and ERβ in mouse foetal lung tissues and identify possible gender differences. The foetal lung tissue was collected between developmental days E15-E19, processed for histology and the expression of oestrogen receptors was detected by immunohistochemistry. Over the 5 days of lung development that were evaluated ERα was not expressed in the foetal lung tissues of neither male nor female mice. In contrast, ERβ was detected in both sexes, although the immunoreactivity differed for each developmental day whilst the staining intensity observed for ERβ also indicated differences between male and female lung tissues. The results demonstrate the existence of a gender difference in the foetal expression of ERβ in lungs of mice.

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“…In recent years, studies investigating the expression and function of ERβ have proven helpful for elucidating the mechanism by which ERβ functions . In our study, we investigated the expression of ERβ (especially ERβ1 and ERβ2) in LADE of mice, consistent with the previous studies . FQ‐PCR showed that the mRNA expression of ERβ and ERβ2 in the E2 group was significantly higher than that in the E2+Ful group and control group.…”

Selecting the most appropriate time points to profile in high-throughput studies

“…Evaluation of the TPS profile suggests that the Esr2 decreases briefly after birth, followed by an increase from around day 5 until day 20 whereas non-optimized profile suggests a relatively flat profile. While fetal mouse lungs express both Esr2 alpha and beta, adult mouse lungs express only Esr2 beta consistent with the TPSresults ( Carvalho and Goncalves, 2012 ). Igfbp3 : Insulin-like growth factor binding protein 3 ( Igfbp3 ) belongs to the Igfbp family and has a Igfbp domain and a thyroglobulin type-I domain ( http://www.ncbi.nlm.nih.gov/gene/3486 ).…”

“…Evaluation of the TPS profile suggests that the Esr2 decreases briefly after birth, followed by an increase from around day 5 until day 20 whereas non-optimized profile suggests a relatively flat profile. While fetal mouse lungs express both Esr2 alpha and beta, adult mouse lungs express only Esr2 beta consistent with the TPSresults ( Carvalho and Goncalves, 2012 ).…”

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