Immunohistological patterns of blood group ABO and type 1 chain (Lewis a Lewis b) and type 2 chain (H‐2, Y) antigens in normal uterine cervix

Torrado, Julie; Hoyos, Ariel Gutierrez; Blasco, E.; Larraz, Juan; Rivas, Jose Luis Fernandez

doi:10.1111/j.1399-0039.1990.tb01791.x

Cited by 8 publications

(4 citation statements)

References 11 publications

(7 reference statements)

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“…In addition sections of cervical tissues with known ABO, Lewis blood group and saliva secretor status served as positive and negative tissue controls (Torrado et al 1990). In addition sections of cervical tissues with known ABO, Lewis blood group and saliva secretor status served as positive and negative tissue controls (Torrado et al 1990).…”

Section: Methodsmentioning

confidence: 99%

Expression of type-2 histo-blood group carbohydrate antigens (Lex, Ley, and H) in normal and malignant human endometrium

Ravn

Mandel

Svenstrup

et al. 1994

Vichows Archiv A Pathol Anat

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Changes in expression of histo-blood group ABH and Lewis antigens are common alterations in carcinomas. Using immunohistochemistry, we have evaluated the expression of type-2 histo-blood group antigens in normal and malignant endometrial tissues in relation to genetic and hormonal factors. The Le(x), sialosyl-Le(x), and Le(y) antigens were inconstantly expressed in the normal endometrium. The expression was uninfluenced by the secretor status but was related to the ABO blood group status in Oestradiol (E2) stimulated endometria. Le(y) was expressed most frequently in proliferating endometria from blood group 0 individuals. Le(x) and Le(y) were maximally expressed in atrophic endometria, and Le(x) and Le(y) staining scores correlated inversely with serum levels of E2 in normal, non-secretory endometria. No correlation was found in adenomatous hyperplasias and endometrial carcinomas, which when compared with atrophic endometria, showed a loss of Le(x) and Le(y) and an increased H-carbohydrate expression at apical membranes. Carcinomas from non-secretors showed lower expression of Le(y) and H-antigens than carcinomas from secretors. Our findings suggest that the genetic and hormonal influence on glycosylation based on type-2 chain carbohydrates differ between normal and malignant endometrium. This difference is probably related to specific tumour-associated qualitative and quantitative changes in the fucosyltransferases.

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Section: Methodsmentioning

confidence: 99%

Expression of type-2 histo-blood group carbohydrate antigens (Lex, Ley, and H) in normal and malignant human endometrium

Ravn

Mandel

Svenstrup

et al. 1994

Vichows Archiv A Pathol Anat

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“…This glycan is modifiable to other glycans including Lewis and ABO blood group antigens 21 (Supplementary fig. 2a).…”

mentioning

confidence: 99%

An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells

et al. 2011

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A significant risk in the clinical application of human pluripotent stem cells (hPSCs), including human embryonic and induced pluripotent stem cells (hESCs and hiPSCs), is teratoma formation from residual undifferentiated cells. We have raised a monoclonal antibody (mAb) against hESCs, designated SSEA-5, which binds a novel antigen highly and specifically expressed on hPSCs--the H type-1 glycan. Separation of SSEA-5 high cells through fluorescence-activated cell sorting (FACS) drastically reduced teratoma formation potential. To ensure complete removal we identified additional markers exhibiting a large dynamic expression range during differentiation: CD9, CD30, CD50, CD90, and CD200. Immunohistochemistry (IHC) conducted on human fetal tissues and bioinformatics analysis of a microarray database revealed that concurrent expression of these markers is both common and specific to hPSCs. When applied to incompletely differentiated hESC cultures, immunodepletion with SSEA-5 and 2 additional markers completely removed teratoma formation potential.

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“…Staining was controlled by replacing the primary antibody with diluent buffer, a mAb of irrelevant specificity but with the same isotype, and culture supernatant. Cervical tissues with known ABO, Le blood-group and saliva-secretor status served as positive and negative tissue controls [40]. Neuraminidase treatment was controlled as previously described [30,31,32], Staining was scored semi-quantitatively in arbitrary staining scores as follows: score 0, no stain; score 1, <10% of the cells stained; score 2 between 10% and 25%; score 3 between 25% and 75%; score 4 >75% of the cells stained.…”

Section: Methodsmentioning

confidence: 99%

Type-1 chain histo-blood group antigens (Lea, monosialosyl-Lea, disialosyl-Lea, Leb, and H) in normal and malignant human endometrium

Ravn

Mandel

Dabelsteen

et al. 1994

Vichows Archiv A Pathol Anat

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Type-1 chain histo-blood group antigens such as the Lewis (Le)a, monosialosyl-Le(a), Le(b) and H antigens show an increased expression in endometrial carcinomas. However, the possibility that these antigens are expressed under genetic or hormonal influence in endometrial carcinomas has not been considered. In the present study, the expression of type-1 chain carbohydrate antigens in normal and malignant endometrium was evaluated by immunohistochemistry and related to both genetic and hormonal factors. The glands of normal, non-secretory endometria expressed, in contrast with surface epithelial cells, Le(a), Le(b), disialosyl-Le(a), and H determinants infrequently. Adenomatous hyperplasias and endometrial carcinomas showed an increased expression of type-1 chain carbohydrates that was qualitatively influenced by the erythrocyte Lewis phenotype and the secretor status. Whereas Le(a+b-) non-secretors mainly accumulated Le(a) antigen, and only limited amounts of Le(b) antigen, Le(a-b+) secretors expressed H, Le(b) and Le(a) antigens. The expression of type-1 chain antigens showed no association with the serum-oestrogen level or to the hormone-receptor status. Thus the Lewis secretor status has a qualitative influence on the increased expression of type-1 chain antigens, which, however, seem to be unrelated to hormonal factors. Our findings suggest an increased activity of the Se-gene-defined or a closely related fucosyl-transferase in neoplastic endometrial epithelial cells.

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Immunohistological patterns of blood group ABO and type 1 chain (Lewis a Lewis b) and type 2 chain (H‐2, Y) antigens in normal uterine cervix

Cited by 8 publications

References 11 publications

Expression of type-2 histo-blood group carbohydrate antigens (Lex, Ley, and H) in normal and malignant human endometrium

Expression of type-2 histo-blood group carbohydrate antigens (Lex, Ley, and H) in normal and malignant human endometrium

An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells

Type-1 chain histo-blood group antigens (Lea, monosialosyl-Lea, disialosyl-Lea, Leb, and H) in normal and malignant human endometrium

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