Immunohistological diagnosis of central nervous system tumours using a monoclonal antibody panel.

Coakham, H. B.; Garson, Jeremy A.; Allan, Patricia; Harper, Ei; Brownell, Betty; Kemshead, J. T.; Lane, E. Birgitte

doi:10.1136/jcp.38.2.165

Cited by 51 publications

(13 citation statements)

References 16 publications

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“…It was recently estimated that there are at least 65 functional intermediate filament genes in the human genome, of which 54 are keratins 17. Like other intermediate filaments, keratins are characterized by tissue‐specific expression patterns which make them useful tools for diagnostic pathology 18–20. The basic structure of single keratin polypeptides is illustrated in Figure 1, but this overall structure could apply equally to any other intermediate filament protein.…”

Section: Phenotypic Variation Between Different Keratin Genesmentioning

confidence: 99%

Keratins and skin disorders

Lane

McLean

2004

The Journal of Pathology

214

188

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The association of keratin mutations with genetic skin fragility disorders is now one of the best-established examples of cytoskeleton disorders. It has served as a paradigm for many other diseases and has been highly informative for the study of intermediate filaments and their associated components, in helping to understand the functions of this large family of structural proteins. The keratin diseases have shown unequivocally that, at least in the case of the epidermal keratins, a major function of intermediate filaments is to provide physical resilience for epithelial cells. This review article reflects on the variety of phenotypes arising from mutations in keratins and the reasons for this variation.

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Section: Phenotypic Variation Between Different Keratin Genesmentioning

confidence: 99%

Keratins and skin disorders

Lane

McLean

2004

The Journal of Pathology

214

188

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“…Monoclonal antibodies are increasingly used in the investigation of CNS tumours, notably neuroblastomas and gliomas [1,2,3]. Whilst establishing a polyclonal and monoclonal antisera panel for typing primary CNS and metastatic tumours we evaluated the suitability of a monoclonal to epithelial membrane antigen (EMA) for inclusion in this panel.…”

Section: Introductionmentioning

confidence: 99%

Cross reactivity of anti-epithelial membrane antigen monoclonal for reactive and neoplastic glial cells

Hitchcock

Morris

1987

J Neuro-Oncol

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“…Cultured astrocytomas that are AJ8-/AO10+/GFA + represent more differentiated cell lines; those that are AJ8+/ AO10-/GFA-represent less differentiated cell lines; and those that are AJ8-/AO10+/GFA -represent a group at an intermediate stage of differentiation. A panel of MAbs has been used to characterize and/or diagnose tumors of the central nervous system [28,31]. MAb Ep-C4 may contribute to developmental studies of brain tumors.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibody against ependymoma-derived cell line

et al. 1992

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Mouse myeloma cells were fused with spleen cells from mice that had been immunized with a human ependymoma derived cell line, KMS II. Hybridomas producing monoclonal antibodies (MAbs) were screened and cloned. Specificity of the antibody was determined by enzyme-linked immunosorbent assay (ELISA) and/or indirect immunofluorescence assay. One of the MAbs, designated Ep-C4 (subclass = IgG1), reacted with two cell lines derived from ependymoma but did not react with 17 cell lines derived from other types of brain tumor nor with 4 neuroblastoma cell lines or 19 cell lines derived from carcinoma, hematopoietic tumors and amnion. Indirect immunofluorescence and immuno-electron microscopy studies revealed that the antigen recognized by MAb Ep-C4 was located on cell surface membrane. The membrane antigen of KMS II cells, immunoprecipitated by MAb Ep-C4, was a protein of 81,000 dalton. The reactivity of MAb Ep-C4 was further examined using immunofluorescence and/or immunoperoxidase methods and frozen sections and short-term cultures of various types of brain tumors. No cross-reactivity with normal adult or fetal brain tissues was detected by absorption assay and immunoperoxidase staining. Our results suggest that the antigen defined by MAb Ep-C4 is specific for ependymoma cells, and different from the antigens of glioma cells or other neuroectodermal-derived cells previously described.

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Immunohistological diagnosis of central nervous system tumours using a monoclonal antibody panel.

Cited by 51 publications

References 16 publications

Keratins and skin disorders

Keratins and skin disorders

Cross reactivity of anti-epithelial membrane antigen monoclonal for reactive and neoplastic glial cells

Monoclonal antibody against ependymoma-derived cell line

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