Cross reactivity of anti-epithelial membrane antigen monoclonal for reactive and neoplastic glial cells

Hitchcock, Edward; Morris, Christine S.

doi:10.1007/bf00195605

Cited by 18 publications

(5 citation statements)

References 19 publications

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“…It is mainly confined to the luminal membrane of normal glandular epithelium (29) and is a good marker of epithelial differentiation (9). EMA positivity in ependymomas has been observed by some investigators (6,12) but others have reported negative results (25,29). Only one tumour in our study showed immunoreactivity to EMA.…”

Section: Discussionsupporting

confidence: 49%

Tumours in Iceland 15. Ependymoma

Birgisson

Blöndal

Björnsson

et al. 1992

APMIS

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Thirteen ependymomas reported to the Icelandic Cancer Registry during a 32‐year period (1955–1986) were histologically reviewed and reclassified according to the WHO Histological Typing of Tumours of the Central Nervous System. The annual incidence rate of ependymoma was 0.20/100.000. Clinical observations and data on biological behaviour and immunohistochemistry are presented. Four tumours were supratentorial, six infratentorial and three intraspinal. There were ten males and three females with a mean age of 32 years (range 2.5–68). The mean postoperative survival of nine surgically treated patients was 35.5 months. Histologically, eight tumours were classical ependymomas, three anaplastic and two myxopapillary. Of 11 tumours stained for GFAP, nine were positive. Nine of 10 tumours tested were positive for vimentin, five for NSE and four for S‐100. None of the 10 tumours showed reactivity with AFP, CEA, chromogranin, desmin, factor VIII, keratin or neurofilament.

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Section: Discussionsupporting

confidence: 49%

Tumours in Iceland 15. Ependymoma

Birgisson

Blöndal

Björnsson

et al. 1992

APMIS

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“…To our knowledge, up until 1986 EMA had not been identified in normal nervous tissue (Ellis et al, 1984) nor i n neuroectodermal brain tumours; including cells in two ependymomas (Sloane & Ormerod, 1981;Cordell et al, 1985). However, in a more recent report (Hitchcock & Morris, 1987), reactive and neoplastic glial cells, including cells i n two ependymomas, were shown to be positive for EMA.…”

Section: Introductionmentioning

confidence: 82%

Epithelial Membrane Antigen Expression in Ependymomas

et al. 1988

Neuropathology Appl Neurobio

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Twenty-seven ependymomas were studied (18 'benign' or low grade and nine 'malignant' or high grade) by means of a monoclonal antibody to epithelial membrane antigen (E29) and an antiserum to glial fibrillary acidic protein (GFAP). The E29 antibody reacted with 'benign' ependymomas but not with 'malignant' ones. Staining was located on the cell surface and especially that facing rosette lumina. Cells forming papillary structures and ependymal epithelium showed a similar distribution of staining. Glial fibrillary acidic protein (GFAP) reactivity was seen in all tumours, with a perivascular accentuation in 'malignant' ones. Staining occurred in the cytoplasm of scattered cells and in those forming papillary structures, ependymal epithelium and rosettes. Our results may have implications in relation to the cytogenesis of these tumours and may also be useful in the histological assessment of 'benign' versus 'malignant' ependymomas.

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“…A three-layer immunoperoxidase technique for paraffin sections was used with the monoclonals to vimentin (VIM), cytokeratin (CK) as detailed previously [10]. The same tissue preparation was used for detecting Desmoplakin I & II using a biotin streptavidin technique.…”

Section: Methodsmentioning

confidence: 99%

Immunocytochemistry of intracranial meningiomas

Hitchcock¹,

Morris²

1987

J Neuro-Oncol

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Fifty meningiomas of four major histological types have been examined by immunocytochemical methods applied to formalin fixed paraffin embedded material and using readily available commercial antisera. The expression of GFAP, S100, NSE, vimentin, cytokeratin, fibronectin and desmoplakin was investigated. The majority of tumours contained NSE (86%) and S100 (78%), and all irrespective of histological type, contained vimentin and fibronectin. The epithelial marker cytokeratin was found in 56% of cases, but showed only focal expression. The relevance of these findings to histogenesis and metabolism is discussed and it is suggested that intermediate filament typing provides valuable information on meningiomas structure and function. The possible role of NSE and S100 in meningeal neoplasm is discussed.

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Cross reactivity of anti-epithelial membrane antigen monoclonal for reactive and neoplastic glial cells

Cited by 18 publications

References 19 publications

Tumours in Iceland 15. Ependymoma

Tumours in Iceland 15. Ependymoma

Epithelial Membrane Antigen Expression in Ependymomas

Immunocytochemistry of intracranial meningiomas

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