Immunohistolocalization of Carbonic Anhydrase Isozyme (CA-VI) in Bovine Mammary Glands

Abstract

ABSTRACT. The localization of bovine carbonic anhydrase isozyme VI (CA-VI) was examined immunohistochemically in bovine mammary glands during early lactation period (after 2-3 days of postpartum) and dry period (at about 2 months preparturition in adu lts), and young calves (at 30 and 150 days after birth) using specific CA-VI antiserum. The immunoreaction for anti-CA-VI antiserum was very weak in the mammary glands in young (prepubescent) calves. In dry period, CA-VI was also weakly expressed in secretory epithelial (acinar) and ductal cells. In contrast, the reaction was intense in mammary gland cells in early lactation period. Dot blotting analysis indicated that anti-CA-VI reacted positively to beastings and mature saliva, but weakly or not at all to milk during the dry per iod or calf saliva, respectively. The intense expression of CA-VI in the mammary glands in early lactation period might compensate for low levels of secretion from functionally and structurally immature salivary glands in young calves. KEY WORDS: bovine mammary gland, CA-VI, immunohistochemical localization.

The Expression of Carbonic Anhydrase in Canine Mammary Gland and Mammary Gland Tumor

“…CA may play an important role in the development of mammary gland and milk secretion. In fact, it has been reported that the distribution, expression, and concentration of CA VI in bovine mammary gland and colostrum have been reported [6]. In this study the expression of CA II, IV and IX were shown in the normal mammary gland tissue.…”

Immunohistochemical Localization and Gene Expression of Carbonic Anhydrase Isoenzymes CA-II and CA-VI in Canine Lower Airways and Lung

“…, Motoharu OISHI 1) , Tomoko AMASAKI 4) , Satoshi SOETA 5) , Nobutsune ICHIHARA 1) , Toshiho NISHITA 2) , Masaru MURAKAMI 3) , Hajime AMASAKI 5) and Masao ASARI 1) * 1) Departments of Anatomy, 2) Carbonic anhydrase (CA; EC4.2.1.1) is a zinc-containing metalloenzyme that has many functions, including macromolecule stabilization, gas exchange, acid-base balance, ion transport, and bone resorption [17]. About 14 CA isoenzymes are widely distributed in almost all mammalian species [4].…”

“…CA-II is the most common CA family and these enzymes exhibit high catalytic activity in erythrocytes, liver, pancreas and kidney cells [15], and in the cells of the parotid and submandibular glands [13]. CA-VI is one of the secreted-type CAs, and has been detected in the salivary glands [6], mammary glands [5] and lacrimal glands [11].…”

Immunohistolocalization and Gene Expression of Secretory Carbonic Anhydrase Isoenzyme CA-VI in Canine Nasal Cavity

“…Of these isoenzymes, only CA-VI occurs in secreted form, and it localizes to the digestive organs, specifically the parotid, submandibular and esophageal, lingual serous von Ebner's glands in sheep [8,9,31], rats [7,18,23], humans [13,18,20,24,29] and dogs [2,14,21]. Furthermore, the CA-VI isoenzyme has been identified in the lacrimal and mammary glands of rats [26], sheep [25] and cattle [12,16]. The CA-VI secreted in mammary glands regulates acid-base balance of upper alimentary canal [12].…”

“…Furthermore, the CA-VI isoenzyme has been identified in the lacrimal and mammary glands of rats [26], sheep [25] and cattle [12,16]. The CA-VI secreted in mammary glands regulates acid-base balance of upper alimentary canal [12]. Highly concentrated buffer ions are important for mucosal protection against an excessively acidic milieu.…”