Immunohistochemical visualization of insulin receptors in formalin-fixed bovine ovaries post mortem and in granulosa cells collected in vivo

Bossaert, Peter; Cock, H. De; Leroy, J.L.M.R.; Campeneere, Sam De; Bols, P.E.J.; Filliers, Muriel; Opsomer, G.

doi:10.1016/j.theriogenology.2010.01.012

Cited by 22 publications

(16 citation statements)

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“…This classification, however, is retrospective, and cannot be used, for example, to choose which follicles will be sampled. Moreover, despite a general consensus about the use of a E2/P4 ratio >1 as a threshold to classify large follicles as viable or atretic [7,16], there is a huge variation both in estradiol values (26 to 1,776 ng/mL), as well as in the estradiol: progesterone ratio (1.2 to 106) in follicles >8 mm that have been deemed as viable [20]. These differences suggest possible variations in the developmental status or potential of these follicles and suggest that the E2/P4 ratio has limited power as a criterion for selection of follicles for GC studies.…”

Section: Discussionmentioning

confidence: 99%

In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

Arashiro

Palhão

Wohlres-Viana

et al. 2013

Reprod Biol Endocrinol

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BackgroundMost studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters.MethodsIn the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle.ResultsIn Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/−4.0%, 12.4+/−4.3% and 0.0+/−0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/−58,122 vs 458,095+/−36,407 for Holstein and Gir, respectively; P < 0.05).ConclusionsThe adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses.

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Section: Discussionmentioning

confidence: 99%

In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

Arashiro

Palhão

Wohlres-Viana

et al. 2013

Reprod Biol Endocrinol

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“…Among the endocrine factors, insulin is a crucial hormone for follicular development [127], granulosa cell function, and ovulation [131]. Additionally, insulin may regulate various intracellular processes in the follicle, such as amino acid transport, lipid metabolism, gene transcription, and protein synthesis [132].…”

Section: Reviewmentioning

confidence: 99%

“…Additionally, insulin may regulate various intracellular processes in the follicle, such as amino acid transport, lipid metabolism, gene transcription, and protein synthesis [132]. Insulin acts through its own receptor, which first appears in the granulosa cells of small bovine antral follicles [131]. Insulin receptor is widely distributed throughout all ovarian compartments, including granulosa and theca cells and stromal tissue [127, 131].…”

Section: Reviewmentioning

confidence: 99%

“…Insulin acts through its own receptor, which first appears in the granulosa cells of small bovine antral follicles [131]. Insulin receptor is widely distributed throughout all ovarian compartments, including granulosa and theca cells and stromal tissue [127, 131]. In addition, the concentrations of insulin in follicular fluid are constant at all follicular developmental stages [127].…”

Section: Reviewmentioning

confidence: 99%

“…High levels of insulin receptor mRNA expression in granulosa cells of preovulatory follicles seem to be necessary for development of the ovulatory stage [127]. The presence of insulin receptor in small antral follicles, together with its absence in preantral follicles, indicates the involvement of insulin, and the acquisition of its receptor, during early follicular growth in bovine [131]. This hypothesis has been supported by the fact that increased dietary intake of insulin was associated with recruitment of small follicles (<4 mm), but did not affect follicle selection (medium: 4-8 mm) or dominant (large: >8 mm) follicles [134].…”

Section: Reviewmentioning

confidence: 99%

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In vitro culture of bovine preantral follicles: a review

Araújo

Gastal

Figueiredo

2014

Reprod Biol Endocrinol

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Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral follicles and formation of early antral follicles from large (secondary) preantral follicles after in vitro culture. Therefore, this review will highlight the basic aspects of bovine folliculogenesis by focusing on preantral follicles, the methods of harvesting preantral follicles, the main results from in vitro follicular culture during the last 20 years, and the potential candidate substances (basic supplements, growth factors, and hormones) for improving the efficiency of in vitro follicle growth.

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The Importance of the Periconception Period: Immediate Effects in Cattle Breeding and in Assisted Reproduction Such as Artificial Insemination and Embryo Transfer

Eetvelde

Heras

Leroy

et al. 2017

Periconception in Physiology and Medicine

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In livestock breeding, the successful outcome is largely depending on the "periconception environment" which, in a narrow sense, refers to the genital tract, where gametogenesis and embryogenesis occur. During these early stages of development, gametes and embryos are known to be particularly sensitive to alterations in their microenvironment. However, as the microenvironment somehow reflects what is going on in the external world, we must widen our definition of "periconception environment" and refer to all events taking place around the time of conception, including metabolic state and health and nutrition of the dam. In modern dairy cows that have to manage an optimal reproductive performance with continued growth and high milk yield, the periconception period is particularly challenging. The metabolic priority for growth and lactation is known to generate adverse conditions hampering optimal ovarian function, oocyte maturation, and development of embryo/fetus. In addition, by using artificial reproductive technologies (ARTs), gametes and/or embryos of livestock are exposed to unnatural conditions outside the male and female genital tract. Artificial insemination, the most widely used technique, is currently yielding pregnancy rates similar to natural mating, and calves produced by AI are equally viable after natural mating. In contrast, other ART, such as multiple ovulation and embryo transfer, have been reported to induce changes in gene expression and DNA methylation patterns with potential consequences for development.Finally, the "periconceptional" environment has been shown to not only influence the successful establishment of pregnancy but also the long-term health and productivity of the offspring. Hence, the optimization of management around the time of conception might open doors to improve animal production and product quality.

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Immunohistochemical visualization of insulin receptors in formalin-fixed bovine ovaries post mortem and in granulosa cells collected in vivo

Cited by 22 publications

References 29 publications

In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system

In vitro culture of bovine preantral follicles: a review

The Importance of the Periconception Period: Immediate Effects in Cattle Breeding and in Assisted Reproduction Such as Artificial Insemination and Embryo Transfer

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