Immunohistochemical Study of the Distributions of the Peptide- and Catecholamine-Containing Nerves in the Omasum of the Sheep

Yamamoto, Yoshio; Kitamura, Nobuo; Yamada, Junzo; Yamashita, T

doi:10.1159/000147564

Cited by 19 publications

(18 citation statements)

References 4 publications

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“…This is the first demonstration of TH-IR in neurons and fibres in the sheep digestive apparatus. In the investigation of Yamamoto et al (1994) in adult sheep omasum, TH immunoreactivity has been detected only in nerve fibres. A subpopulation of vagal-crest derived cells in the mouse ENS transiently express TH immunoreactivity (Young et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

et al. 2004

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We have identified the enteric neuron types expressing immunoreactivity for the calcium-binding protein calbindin D28k (CALB) in cryostat sections and whole-mount preparations of myenteric (MP) and submucosal (SMP) plexuses of sheep ileum. We wished to determine whether CALB-IR in the sheep enteric nervous system was expressed in Dogiel type II cells, as in guinea-pig and rat ileum, and could therefore be used as a marker for intrinsic primary afferent neurons. The neurochemical coding of CALB-containing myenteric and submucosal neurons in ileum of unweaned lamb and mature sheep and its co-localisation with various neural markers was studied immunohistochemically. An antiserum against neuronal nuclear protein (NeuN) failed to detect the entire neuronal population; it was expressed only in 48% of neuron-specific enolase (NSE)-immunoreactive (NSE-IR) neurons. Human neuronal protein appeared to occur in the large majority or all neurons. Almost all CALB-IR neurons were: (1) radially multidendritic; (2) eccentric multidendritic; (3) Dogiel type II. CALB-IR occurred in 20-25% of myenteric and 65-75% of submucosal neurons in lamb and mature sheep, with higher values in mature sheep. Nearly all CALB-IR neurons were common choline acetyltransferase (cChAT)-IR, whereas only about 20% of cChAT-IR somata were CALB-IR. In lamb and mature sheep, 90% of MP CALB-IR neurons were peripheral choline acetyltransferase (pChAT)-IR. In lamb SMP, 80+/-13% of CALB-IR cells were also pChAT-IR, whereas all those in mature SMP were pChAT-IR. Fewer myenteric CALB-IR neurons exhibited tachykinin (TK) in mature sheep (49%) than in lamb (88%). This was also the case for submucosal ganglia (mature sheep, 63%; lamb, 89%). In lamb MP, 77+/-7% of CALB-IR cells were NeuN-positive. In mature sheep, 73+/-10% of CALB-IR somata were NeuN-IR, but NeuN failed to stain SMP neurons. In the MP of suckling and mature sheep, Dogiel type II CALB-IR neurons were calcitonin gene-related peptide (CGRP)-IR. In the SMP at both stages, Dogiel type II CALB-IR somata (about 50% of CALB-IR neurons) were also CGRP-IR. Only small proportions of CALB-IR neurons showed immunoreactivity for calretinin or nitric oxide synthase (NOS), although large populations of CALB and NOS neurons occurred in the ganglia. Thus, CALB is a marker of most Dogiel type II neurons in the sheep but is not confined to Dogiel II neurons. CGRP is a more selective marker of Dogiel type II neurons, being only found in this neuron type.

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Section: Discussionmentioning

confidence: 99%

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

et al. 2004

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“…In previous immunohistochemical studies, neuropeptides [15,16,17,25,26,27,38], glial cells [36], and endocrine cells [2] were detected in the forestomach of adult ruminants. However, little research examining the prenatal development of these cell types in the stomach of ruminants such as sheep [37], deer [6,7,9,10,28,29], and goats has been conducted [11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical evaluation of the goat forestomach during prenatal development

et al. 2014

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Here we report the detection and distribution of synaptophysin (SPY), non-neuronal enolase (NNE), glial fibrillary acidic protein (GFAP), vimentin (VIM), neuropeptide Y (NPY), and vasoactive intestinal peptide (VIP) expression in the goat forestomach during prenatal development. A total of 140 embryos and fetuses were examined to evaluate protein expression from the first stage of prenatal life until birth. In all cases, SPY immunoreactivity was detected at 53 days gestation in the lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexuses. Immunoreactivity to NNE was observed at 64 days gestation in the same locations as well as the epithelial layer. Glial cells were found at 64 days as indicated by signals corresponding to GFAP and VIM at 39 days. Positive staining for NPY and VIP was observed at 113, 75, and 95 days in the rumen, reticulum, and omasum, respectively, in the lamina propria-submucosa, tunica muscularis, and myenteric plexuses of each of these gastric compartments. These findings indicate possible preparation of the fetal goat forestomach for postnatal function. Compared to other ruminant species, neuroendocrine cells, glial cells and peptidergic innervations markers were detected earlier compared to sheep but at around the same stage as in deer.

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“…Vasoactive intestinal polypeptide receptors are present in the mucosa, submucosa, smooth muscle layers and the neural plexuses in the gastrointestinal tract, and are particularly dense in the region of the reticulo-omasal orifice (ROO) and in the muscle layer of the omasal wall (Yamamoto et al 1994). Vasoactive intestinal polypeptide is involved in the relaxation process of the ROO in fasted and milk-fed lambs in a warm environment (Reid et al 1988a,b) and in cold acclimatized sheep .…”

Section: Mots Clésmentioning

confidence: 99%

Effect of vasoactive intestinal polypeptide and environmental temperatures on feed intake, digesta flow and nutrient digestion in sheep

Li¹,

Okine²,

Christopherson³

2002

Can. J. Anim. Sci.

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. 2002. Effect of vasoactive intestinal polypeptide and environmental temperatures on feed intake, digesta flow and nutrient digestion in sheep. Can. J. Anim. Sci. 82: [165][166][167][168][169][170][171][172]. Three experiments were conducted to test the hypothesis that vasoactive intestinal polypeptide (VIP) infused into the gastric artery and exposure to a low temperature will increase feed intake, duodenal flow and amino acid supply to the small intestine of sheep, both effects as a result of increased ruminal outflow. A total of eight sheep (55 ± 2.7 kg) with rumen and duodenal cannulae and eight sheep without digestive cannulae were provided with continuous access to a diet of alfalfa pellets. Water and salt licks were accessible 24 h d -1 . Two infusion treatments, VIP (1 nmol min -1 ) solution or a saline control (0.9% NaCl solution) were given through a left gastricartery catheter for 10 min each hour for the first 3 h after feeding each day for 3 to 4 consecutive days in a crossover arrangement during experimental periods. Sheep were exposed to 1 ± 2°C in exp. 1, to a thermoneutral (21 ± 2°C) environment in exp. 3 and to both thermoneutral (21 ± 1.5°C) and cold environments (1 ± 2°C) in exp. 2. These studies demonstrated that a VIP infusion of short duration did not increase dry matter intake (DMI) (P > 0.05) and did not influence duodenal passage of DM, OM, CP, amino acids (AA) or bacterial protein (P > 0.05). The exposure of animals to a cold environment stimulated (P < 0.05) intake of DM, OM and AA and duodenal passage of DM, OM, AA and microbial protein. There was an interaction (P < 0.05) between VIP and environmental temperature such that VIP improved duodenal AA passage in the thermoneutral, but not in the cold, environment. The data suggest that short-term VIP treatment does not induce a change in feed intake when sheep have access to feed for 24 h d -1 . Cold environmental temperature stimulates significant increases in feed intake and passage of protein and AA into the small intestine of sheep fed ad libitum, such that there was enrichment of intestinal supply of AA relative to digestible organic matter. Les animaux avaient aussi accès à de l'eau et à des blocs à lécher 24 heures par jour. Du PIV (1 nmol par minute) et de la saline (solution de NaCl à 0,9 %), comme solution témoin, ont été administrés aux animaux au moyen d'un cathéter inséré dans l'artère gastrique 10 minutes par heure pendant les 3 premières heures suivant l'alimentation, 3 ou 4 journées d'affilée, dans le cadre d'un plan croisé. Les animaux ont été exposés à une température de 1 ± 2°C lors de la première expérience, à une température neutre (21 ± 2°C) lors de la troisième et aux deux températures lors de la deuxième (température neutre de 21 ± 1,5°C et température froide de 1 ± 2°C). Les résultats révèlent qu'une brève infusion de PIV n'augmente pas la prise de matière sèche (MS) (P > 0,05) et n'exerce aucune influence sur le transit de la MS, de la matière organique, des protéines brutes, des acides aminés et des protéines...

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Immunohistochemical Study of the Distributions of the Peptide- and Catecholamine-Containing Nerves in the Omasum of the Sheep

Cited by 19 publications

References 4 publications

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

Immunohistochemical evaluation of the goat forestomach during prenatal development

Effect of vasoactive intestinal polypeptide and environmental temperatures on feed intake, digesta flow and nutrient digestion in sheep

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