Immunohistochemical Study of Secretory Component, Secretory IgA and Carcinoembryonic Antigen in Large Bowel Carcinomas

Rognum, Torleiv O.; Brandtzæg, Per; Orjasaeter, H; Hognestad, J

doi:10.1016/s0344-0338(80)80161-0

Cited by 41 publications

(29 citation statements)

References 41 publications

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“…Fluorescein isothiocyanate (FITC)-labelled sheep anti-SC wNas combined with tetramethylrhodamine isothiocyanate (MRITC)-labelled rabbit anti-CEA; and FITC-labelled rabbit anti-IgA was combined w%!ith MRITC-labelled slheep anti-SC. The preparation and characterization of these sheep and rabbit IgG-fluorochrome conjugates lhave been reported previously (Rognum et al, 1980). A third adjacent section was stained with a trichrome routine rnethod combining haematoxylin, azofloxine and saffron (Stave & Brandtzaeg.…”

Section: Methodsmentioning

confidence: 99%

“…A third adjacent section was stained with a trichrome routine rnethod combining haematoxylin, azofloxine and saffron (Stave & Brandtzaeg. 1977 The fluorescence intensity of thie three epithelial marker antigens (CEA, SC, and IgA) was evaluated wAith the semiquantitative scoring system detailed elsewhere (Rognum et al, 1980). Briefly, a score of 3 indicated strong intensity and 0 negligible or non-staining.…”

Section: Methodsmentioning

confidence: 99%

“…Sections stained for conventional histopathology were randomized and graded using the criteria of Evans (Ashley, 1978); a score of 1 referring to poorly differentiated carcinoma and 5 indicating high differentiation. The same pathologist performed all this grading, and a blind reproducibility control revealed no systematic error (Rognum et al, 1980).…”

Section: Methodsmentioning

confidence: 99%

“…Secretory IgA and secretory component (SC) belong to the first category. Thus, the functional role of SC as an epithelial receptor for dimeric IgA is well documented (Brandtzaeg, 1974(Brandtzaeg, , 1981Brandtzaeg & Baklien, 1977;Brown, 1978) and previous studies have indicated that SC and epithelial IgA may be useful indicators of the degree of differentiation of neoplastic intestinal epithelium Weisz-Carrington et al, 1976;Green et al, 1977;Rognum et al, 1980). The carcinoembryonic antigen (CEA) of Gold & Freedman (1965) represents the second type of epithelial marker.…”

mentioning

confidence: 99%

“…The carcinoembryonic antigen (CEA) of Gold & Freedman (1965) represents the second type of epithelial marker. It has been demonstrated in the glycocalyx region of gastrointestinal epithelial cells (Gold et al, 1968;von Kleist & Burtin, 1969a, b;Denk et al, 1972) but its relation to malignancy is still a matter of controversy (Von Kleist & Burtin, 1969a, b;Denk et al, 1972;Isaacson & Le Vann, 1976;Pihl et al, 1980;Rognum et al, 1980Rognum et al, , 1982aO'Brian et al, 1981;Goslin et al, 1981a).…”

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confidence: 99%

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Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma

Rognum¹,

Thorud²,

Elgjo³

et al. 1982

Br J Cancer

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Summary.-Immunofluorescence staining for carcinoembryonic antigen (CEA), secretory component (SC), and epithelial IgA was evaluated semiquantitatively in 85 large-bowel carcinomas in relation to degree of tumour differentiation, Dukes' stage, and plasma CEA level. The tumours were divided into a near-diploid (ND, 28) and an aneuploid group (AN, 57) by means of flow-cytometric DNA measurements. Expression of SC and IgA in neoplastic epithelium was positively related to differentiation in both groups. The AN tumours scored significantly higher for CEA than the ND ones, but the staining was apparently unrelated to differentiation or Dukes' stage. CEA expression in the transitional mucosa adjacent to ND tumours was negatively correlated with tumour differentiation, whereas epithelial IgA and SC in this zone showed a substantially higher positive correlation with tumour differentiation, and a somewhat stronger negative correlation with Dukes' stage in the ND than in the AN group. Plasma CEA levels were significantly related to Dukes' stage, only in patients with AN tumours, and only in this group were positively correlated with estimates of total tumour CEA for Dukes' stages A and B. For Dukes' stages C and D (disseminated tumours), moreover, the plasma CEA levels were found to be significantly higher in the AN group. These findings indicate that the DNA profile of large-bowel carcinomas is related both to the way neoplastic cells influence the activity of the transitional mucosa and their capacity for expression and release of epithelial products. AN tumours thus seem to be more active as "secretors" of CEA than ND ones.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma

Rognum¹,

Thorud²,

Elgjo³

et al. 1982

Br J Cancer

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Preservation of cytometric DNA distribution and epithelial marker expression after tumor progression of human large bowel carcinomas

Rognum

Thorud

Brandtzæg

1985

Cancer

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Tumor specimens were obtained from seven patients with large bowel carcinomas at operation of the primary neoplasm and by resection of local recurrences or metastases 2 1/2 to 36 months later. All specimens were evaluated with regard to nuclear DNA distribution as measured by flow cytometry and expression of carcinoembryonic antigen (CEA), secretory component, epithelial IgA, and HLA-DR antigens, as determined by immunofluorescence staining of tissue sections. Both the DNA distributions and the immunohistochemical staining patterns were similar in the primary and secondary tumors. These findings are in keeping with a monoclonal or oligoclonal tumor progression in advanced large bowel carcinoma.

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Elevated levels of secretory immunoglobulins A and M in serum of patients with large bowel carcinoma indicate liver metastasis

Kvale

Rognum

Brandtzæg³

1987

Cancer

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Preoperative serum levels of secretory IgA (SIgA) and secretory IgM (SIgM) were quantified by an enzyme-linked immunosorbent assay in 100 patients with large bowel carcinoma. The values obtained were related to tumor characteristics such as Dukes' stage, differentiation, DNA ploidy pattern, expression of secretory component (SC), volume, and plasma level of carcinoembryonic antigen (CEA). Statistical comparison was made with matched controls. Only patients with Stage D tumors showed significantly increased serum levels of SIgA and SIgM (P less than 0.004). The combined diagnostic sensitivity of SIg and CEA in Stage D was 0.94. In patients with liver involvement, serum SIgA and SIgM were elevated in 67% and 53%, respectively. However, circulating SIg was neither correlated with tumor SC expression nor with any other studied variable. Thus, the raised serum SIg levels were apparently not caused by release of SC from the tumors but, instead, by hampered liver function due to hepatic metastasis.

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Immunohistochemical Study of Secretory Component, Secretory IgA and Carcinoembryonic Antigen in Large Bowel Carcinomas

Cited by 41 publications

References 41 publications

Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma

Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma

Preservation of cytometric DNA distribution and epithelial marker expression after tumor progression of human large bowel carcinomas

Elevated levels of secretory immunoglobulins A and M in serum of patients with large bowel carcinoma indicate liver metastasis

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