Elevated levels of secretory immunoglobulins A and M in serum of patients with large bowel carcinoma indicate liver metastasis

Kvale, Dag; Rognum, Torleiv O.; Brandtzæg, Per

doi:10.1002/1097-0142(19870115)59:2<203::aid-cncr2820590204>3.0.co;2-p

Cited by 22 publications

(10 citation statements)

References 24 publications

(3 reference statements)

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“…Pre-operative samples These results have been published previously (Kvale et al, 1987a). In brief, elevated levels of SC were found only in the Dukes' stage D group (P<0.004) (Table II).…”

Section: Patientssupporting

confidence: 82%

“…It was prompted by our recent observation indicating a strong association between elevated serum levels of SIgA and SIgM and liver metastases in patients' colonic carcinomas (Kvale et al, 1987a). We wanted to see whether circulating SC could reveal liver involvement earlier than ordinary clinical followup.…”

Section: Discussionmentioning

confidence: 91%

“…Previous reports have considered SC as a marker for colorectal carcinomas, both in terms of its cytoplasmic expression in tumours (Poger et al, 1976;Rognum et al, 1982;Arends et al, 1984;Brooks & Ernst, 1984) and its appearance in peripheral blood (Homburger et al, 1984;Kvale et al, 1987a). However, tumour expression and the corresponding serum levels of SC in patients with colorectal (Kvale et al, 1987a) and breast (Kvale et al, 1987c) carcinomas are not correlated.…”

Section: Discussionmentioning

confidence: 93%

“…However, tumour expression and the corresponding serum levels of SC in patients with colorectal (Kvale et al, 1987a) and breast (Kvale et al, 1987c) carcinomas are not correlated. This disparity might be explained by relatively minor release of SC from such tumours to blood, and/or by an active hepatic clearance of circulating secretory immunoglobulins as described in mice (Tomana et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, we recently found generally low serum concentrations of total SC in patients with colonic (Kvale et al, 1987a) or breast carcinomas (Kvale et al, 1987c). In cases of liver involvement, however, elevated levels of SIgA and SIgM were often found, regardless of SC expression in the tumours.…”

mentioning

confidence: 91%

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Early detection of liver metastasis in patients with colorectal carcinoma by increased levels of circulating IgA- and IgM-associated secretory component

Kvale

Rognum²,

Brandtzæg³

1987

Br J Cancer

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Summary One hundred patients operated for colorectal carcinoma were followed clinically and with serial blood samples from 5 to 8 years. Levels of secretory component (SC) associated with IgA and IgM in serum were measured and related to Dukes' stage, histological differentiation, tumour expression of SC, and circulating carcinoembryonic antigen (CEA). On the whole, elevated levels of SC in serum were found in 15 of the 20 patients who already had (n = 15), or later developed (n = 5), liver metastasis. Four of the latter 5 patients showed raised SC levels with a 5.5 months median lead time from the first positive serum sample to clinically manifest liver disease. These data are interesting in view of the promising results reported for liver resection in patients with colorectal carcinoma.

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“…Pre-operative samples These results have been published previously (Kvale et al, 1987a). In brief, elevated levels of SC were found only in the Dukes' stage D group (P<0.004) (Table II).…”

Section: Patientssupporting

confidence: 82%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 91%

See 3 more Smart Citations

Early detection of liver metastasis in patients with colorectal carcinoma by increased levels of circulating IgA- and IgM-associated secretory component

Kvale

Rognum²,

Brandtzæg³

1987

Br J Cancer

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Rapid selection of cultured cells with increased expression of a membrane marker (secretory component)

Kvale

Bártek

Sollid

et al. 1988

Intl Journal of Cancer

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This study showed how immunomagnetic monodisperse microspheres (Dynabeads) can be used for rapid selection of stable and homogeneous sublines of cancer cells. The beads were activated with antibody to a specific surface membrane marker. We used a monoclonal antibody (MAb) to human secretory component (SC), the epithelial receptor for polymeric immunoglobulins (pIg). A relatively low number of particles favored rosetting of strongly positive cells. SC-expressing cells were isolated within 30 min from a colonic carcinoma cell line (HT-29.E10). After 13 months of continuous culture the selected cells had a 15 times higher cellular content and a 10 times higher secretory rate of SC than parallel cultures of the original cell line. Immunomagnetic microspheres can be recommended in experimental cancer research, e.g., in order to minimize cellular heterogeneity and to isolate cells with particular surface membrane phenotypes in differentiation studies. The selection method is rapid and simple, and eliminates laborious test systems for conventional subclone screening.

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IgA With altered carbohydrate structure as a tumor‐associated marker in serum of cancer patients

Henslee

Rittenhouse

Matias

et al. 1988

Clinical Laboratory Analysis

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Serum lgAl contains 0-linked carbohydrate structures, whereas other immunoglobulins contain only N-linked structures. An assay was developed which detects lgAl by measuring the DGalP(1-3) DGalNAc structures 0-linked to the lgAl hinge region. Fifty percent of the serum specimens from cancer patients tested were elevated compared to 1% of the total normal serum specimens tested by this assay. The assay combines antibody (antihuman IgA) and lectin and measures the DGalp(1-3)DGalNAc structure in the lgAl molecule accessible to lectin binding. Three lectins were compared-the lectins from Bauhinia purpurea alba, Maclura pomifera, and peanut agglutinin (PNA). PNA in the assay discriminated best between serum specimens from normal subjects and cancer patients and was chosen as the lectin component in the assay. The anti-lgA-PNA enzyme immunoassay was evaluated in a retrospective study of cancer patients. A panel of 845 serum specimens was tested and the results analyzed at three cutoff R values. The R value of a specimen is the ratio of the assay absorbance obtained for that test specimen with respect to the assay absorbance obtained for a pool of normal human sera. At a cutoff R value of 2.38, 1% normals and 26% benigns were elevated. The following cancer specimens had elevated R values: 52% lung, 60% colon, 43% head and neck, 35% breast, 64% kidney, 38% bladder, 43% prostate, 8% ovarian, and 67% pancreas. Further testing showed a significant percentage of serum specimens from patients with cirrhosis, pancreatitis, or ulcerative colitis also had elevated R values. No correlation of this assay was observed with CEA levels. The results suggest that the carbohydrate structure of serum lgAl is altered by malignancies and that measurement of this altered IgA may supplement CEA testing.

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Elevated levels of secretory immunoglobulins A and M in serum of patients with large bowel carcinoma indicate liver metastasis

Cited by 22 publications

References 24 publications

Early detection of liver metastasis in patients with colorectal carcinoma by increased levels of circulating IgA- and IgM-associated secretory component

Early detection of liver metastasis in patients with colorectal carcinoma by increased levels of circulating IgA- and IgM-associated secretory component

Rapid selection of cultured cells with increased expression of a membrane marker (secretory component)

IgA With altered carbohydrate structure as a tumor‐associated marker in serum of cancer patients

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