Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray

Sha, Ailong; Sun, Hushan; Wang, Yiyan

doi:10.1007/s10989-011-9280-x

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…Opioid system is highly conserved in evolution. 496 Limited evidence for presence of enkephalins 497,498 and opioid receptors. [497][498][499][500][501][502] Analgesics modify the animal's response to stimuli that would be painful for a human Not studied Investigation of candidate substances is required using a combination of neurophysiological recording from nociceptive afferents and behavioural studies.…”

Section: What the Directive 2010/63/eu Means For Cephalopod Researchmentioning

confidence: 99%

Guidelines for the Care and Welfare of Cephalopods in Research –A consensus based on an initiative by CephRes, FELASA and the Boyd Group

et al. 2015

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This paper is the result of an international initiative and is a first attempt to develop guidelines for the care and welfare of cephalopods (i.e. nautilus, cuttlefish, squid and octopus) following the inclusion of this Class of ∼700 known living invertebrate species in Directive 2010/63/EU. It aims to provide information for investigators, animal care committees, facility managers and animal care staff which will assist in improving both the care given to cephalopods, and the manner in which experimental procedures are carried out. Topics covered include: implications of the Directive for cephalopod research; project application requirements and the authorisation process; the application of the 3Rs principles; the need for harm-benefit assessment and severity classification. Guidelines and species-specific requirements are provided on: i. supply, capture and transport; ii. environmental characteristics and design of facilities (e.g. water quality control, lighting requirements, vibration/noise sensitivity); iii. accommodation and care (including tank design), animal handling, feeding and environmental enrichment; iv. assessment of health and welfare (e.g. monitoring biomarkers, physical and behavioural signs); v. approaches to severity assessment; vi. disease (causes, prevention and treatment); vii. scientific procedures, general anaesthesia and analgesia, methods of humane killing and confirmation of death. Sections covering risk assessment for operators and education and training requirements for carers, researchers and veterinarians are also included. Detailed aspects of care and welfare requirements for the main laboratory species currently used are summarised in Appendices. Knowledge gaps are highlighted to prompt research to enhance the evidence base for future revision of these guidelines.

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Section: What the Directive 2010/63/eu Means For Cephalopod Researchmentioning

confidence: 99%

Guidelines for the Care and Welfare of Cephalopods in Research –A consensus based on an initiative by CephRes, FELASA and the Boyd Group

et al. 2015

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“…Immuno-reactive Met-enkephalin has been detected in, for instance, crustaceans (Jaros et al, 1985), annelids (Tasiemski et al, 2000) and tunicates (Georges and Dubois, 1984). In addition, Leu-enkephalin and delta opioid receptor immuno-reactivity are found in a mollusk, the octopus (Sha et al, 2012). Moreover, enkephalins influence the physiology of invertebrates, for instance, disrupting reproduction in insects (Kumar et al, 2013) and impacting both heart and immune functioning in molluscs (Tasiemski et al, 2000; Liu et al, 2016).…”

Section: Met-enkephalin In Birdsmentioning

confidence: 99%

Perspectives on Endogenous Opioids in Birds

Scanes

Pierzchała-Koziec

2018

Front. Physiol.

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The present review summarizes the state of knowledge of endogenous opioids in birds. Endogenous opioid peptides acts in a neuromodulatory, hormonal and paracrine manner to mediate analgesic and other physiological functions. These peptides act through specific G-protein coupled receptors. Opioid receptors consist of a family of four closely-related proteins. The three types of opioid receptors are the mu (MOR or μ), delta (DOR or δ), and kappa (KOR or κ) opioid receptor proteins. The role of the fourth member of the opioid receptor family, the nociceptin or orphanin FQ receptor (ORL), is not clear. The ligands for opioid receptors are: β –endorphin (MOR), Met- enkephalin, Leu-enkephalin (DOR) and dynorphin (KOR), together with probably endomorphins 1 and 2. In spite of long history of research on endogenous opioid peptides, there are no studies of endogenous opioids per se in wild birds and few in poultry species. β-endorphin is present in all birds investigated and there is close agreement between the structures of β-endorphin in different birds. Plasma concentrations of β-endorphin are increased by ether stress in geese. There is evidence that β-endorphin plays a role in the control of luteinizing hormone release in chickens. Met-enkephalin is present in tissues such as the retina, hypothalamus, pituitary gland, and adrenals together with circulation of birds. Stresses such as crowding and withholding water increase circulating concentrations of Met-enkephalin in chickens. The structures of chicken dynorphin A and B have been deduced from cDNA. What is missing are comprehensive studies of plasma concentrations and expression of the full array of endogenous opioids in multiple avian species under different situations. Also, what is not known is the extent to which circulating or locally released or intra-cellular Met-enkephalin influence physiological process in birds. Thus, there is considerable scope for investigation of the physiology of endogenous opioids in birds.

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“…In previous studies, we found Leu-enk and Met-enk immunoreactivities in the mantle and foot tissue of Octopus ocellatus (Sha et al 2007(Sha et al , 2012 but it might also be expected in the nervous and digestive systems. The main purpose of this study was to analyze the distribution of Leu-enk immunoreactivity in the nervous and digestive systems of O. ocellatus.…”

mentioning

confidence: 55%

Leucine-enkephalin immunoreactivity is also present in the nervous and digestive systems of the octopus,Octopus ocellatus

Sha

Sun

Wang

2012

Marine and Freshwater Behaviour and Physiology

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Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray

Cited by 11 publications

References 17 publications

Guidelines for the Care and Welfare of Cephalopods in Research –A consensus based on an initiative by CephRes, FELASA and the Boyd Group

Guidelines for the Care and Welfare of Cephalopods in Research –A consensus based on an initiative by CephRes, FELASA and the Boyd Group

Perspectives on Endogenous Opioids in Birds

Leucine-enkephalin immunoreactivity is also present in the nervous and digestive systems of the octopus,Octopus ocellatus

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