Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

Yj, Huang; Ks, Lu

doi:10.1679/aohc.59.433

Cited by 11 publications

(18 citation statements)

References 29 publications

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“…The existence of a similar taste cell type immunoreactive to CGRP was not reported in TBs of other vertebrates. On the contrary, the presence of CGRP-ir fibers afferent to TBs was reported in both amphibians and mammals (Hisa et al, 1992;Shin et al, 1995;Astbä ck et al, 1995Astbä ck et al, , 1997Wakisaka et al, 1996;Huang and Lu, 1996;Yamamoto et al, 1997;Witt and Reutter, 1998;Kusakabe et al, 1998;Huang et al, 2003;Crescimanno et al, 2004;Ogura et al, 2007), but was not observed in lamprey. Apparently, lampreys and jawed vertebrates followed different evolutionary pathways as regards the distribution of CGRP in the taste system.…”

Section: Discussionmentioning

confidence: 85%

Neurochemical characterization of sea lamprey taste buds and afferent gustatory fibers: Presence of serotonin, calretinin, and CGRP immunoreactivity in taste bud bi‐ciliated cells of the earliest vertebrates

Barreiro‐Iglesias

Villar-Cerviño

Villar-Cheda

et al. 2008

J of Comparative Neurology

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Neuroactive substances such as serotonin and other monoamines have been suggested to be involved in the transmission of gustatory signals from taste bud cells to afferent fibers. Lampreys are the earliest vertebrates that possess taste buds, although these differ in structure from taste buds in jawed vertebrates, and their neurochemistry remains unknown. We used immunofluorescence methods with antibodies raised against serotonin, tyrosine hydroxylase (TH), gamma-aminobutyric acid (GABA), glutamate, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), calretinin, and acetylated alpha-tubulin to characterize the neurochemistry and innervation of taste buds in the sea lamprey, Petromyzon marinus L. For localization of proliferative cells in taste buds we used bromodeoxyuridine labeling and proliferating cell nuclear antigen immunohistochemistry. Results with both markers indicate that proliferating cells are restricted to a few basal cells and that almost all cells in taste buds are nonproliferating. A large number of serotonin-, calretinin-, and CGRP-immunoreactive bi-ciliated cells were revealed in lamprey taste buds. This suggests that serotonin participates in the transmission of gustatory signals and indicates that this substance appeared early on in vertebrate evolution. The basal surface of the bi-ciliated taste bud cells was contacted by tubulin-immunoreactive fibers. Some of the fibers surrounding the taste bud were calretinin immunoreactive. Lamprey taste bud cells or afferent fibers did not exhibit TH, GABA, glutamate, or NPY immunoreactivity, which suggests that expression of these substances evolved in taste buds of some gnathostomes lines after the separation of gnathostomes and lampreys.

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Section: Discussionmentioning

confidence: 85%

Neurochemical characterization of sea lamprey taste buds and afferent gustatory fibers: Presence of serotonin, calretinin, and CGRP immunoreactivity in taste bud bi‐ciliated cells of the earliest vertebrates

Barreiro‐Iglesias

Villar-Cerviño

Villar-Cheda

et al. 2008

J of Comparative Neurology

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“…Nagyon sűrű fonatot alkotnak az ízlelőbimbó bázisán, és csak egy-egy olyan rost fi gyelhető meg az ízlelőbimbóban, amelyik eléri a bimbó pórusát is [13]. Számos neuropeptidtartalmú idegrost található intragemmalisan, extragemmalisan és közvetlenül alattuk a lamina propriában [14,15]. In situ hibridizációs technikával (RT-PCR) Shen és mtsai [16] kimutatták, hogy a patkány-ízlelőbimbó receptorsejtjei kolokalizációban tartalmaznak VIP-et és kolecisztokinint (CCK) az alpha-gustducin és T1R2 transduction molekulával.…”

Section: Innervation Of Papilla Vallata In Diabetic Ratsunclassified

“…A különböző kémiai anyagok (sósav, cukor, nátrium klorid, urea) ízérzési ingerküszöbe diabeteses betegnél megváltozik [22,25,26]. Irodalmi adatok igazolják, hogy az ízlelőbimbók degenerálódnak, ha az axonok degenerálódnak, esetleg regenerálódnak, ha beidegzésük visszaáll [14,27,28]. Ichimori és mtsai [1] kimutatták, hogy mindkét oldali nervus glossopharyngeus denerválása után az ízlelő-bimbók receptorsejtjeiben kétszer több az apoptotikus sejtek mennyisége, egyes esetekben az ízlelőbimbók teljesen el is tűntek patkányban.…”

Section: Megbeszélésunclassified

Innervation of papilla vallata in diabetic rats

et al. 2013

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Bevezetés: A diabetes mellitusban szenvedő betegek 10%-a kóros érzésről panaszkodik, amelyek között a leggyakoribb a fájdalom és az ízérzés zavara. Cél: A papilla vallatában lévő különböző neuropeptidtartalmú idegrostok változá-sainak vizsgálata diabeteses patkányokban. Módszerek: A szerzők streptozotocinnal indukált diabeteses patkányok-ban immunhisztokémiai módszerrel vizsgálták a papilla vallatában lévő neuropeptidtartalmú idegrostok mennyiségi változását. Eredmények: A substance P, galanin, neuropeptid-Y és vasoactiv intestinalis polipeptid tartalmú idegrostok mennyisége szignifi kánsan megemelkedett (p<0,05) két héttel a kezelés után a nyelv nyálkahártyájában. A lymphocy ták és hízósejtek száma szintén szignifi kánsan megemelkedett. Az immunreaktív idegrostok főleg a hám alatt, a tunica propriában helyezkedtek el, de néhány rost behatolt a hámba is, és megfi gyelhetők voltak intergemmalisan és intragemmalisan. A receptorsejtek nem mutattak immunjelzést az általuk vizsgált neuropeptidekre. Neuropeptid-Y és vasoactiv intestinalis polipeptid immunreaktív rostokat nem sikerült az ízlelőbimbókban kimutatni. Négy héttel a streptozotocin alkalmazása után a substance P, calcitonin gene related peptid és galanin immunreaktív rostok száma csökkent mind intergemmalisan, mind intragemmalisan. Amennyiben azonnal elkezdték az inzulinkezelést, akkor az immunreaktív idegrostok száma hasonló volt a kontrollhoz, azonban, ha csak a diabetes megjelenése után egy héttel kezdték el az inzulinkezelést, akkor az idegrostok mennyisége csökkent. Morfológiailag a papilla vallata nagyságában nem találtak különbséget a kontroll-és diabeteses patkányok között, de diabeteses patkányok-ban kevesebb ízlelőbimbót észleltek. Következtetés: Az immunreaktív idegrostok és hízósejtek megemelkedett száma valószínűleg a neurogén gyulladás következménye, ami maga után vonhatja a szájnyálkahártya károsodását és vasoconstrictiót okozhat. Az ízérzés zavarait a négy hete fennálló diabetes esetén az idegrostok degenerációja és szám-beli csökkenése okozhatja. Orv. Hetil., 2013, 154, 443-448. Kulcsszavak: papilla vallata, neuropeptidek, immunhisztokémia, diabetes mellitus Innervation of papilla vallata in diabetic ratsIntroduction: Abnormal sensations such as pain and impairment of taste are symptoms of approximately 10% of patients having diabetes mellitus. Aim: The aim of the study was to investigate and quantify the different neuropeptide containing nerve fi bres in the vallate papilla of the diabetic rat. Methods: Immunohistochemical methods were used to study the changes of the number of different neuropeptide containing nerve terminals located in the vallate papillae in diabetic rats. Diabetes was induced in the rats with streptozotocin. Results: Two weeks after streptozotocin treatment the number of the substance P, galanin, vasoactive intestinal polypeptide and neuropeptide Y immunoreactive nerve terminals was signifi cantly increased (p<0.05) in the tunica mucosa of the tongue. The number of the lymphocytes and mast cells was also increased signi...

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“…Iwanaga et al (1992) have shown that the structure, metabolism, and functional characteristics of paraneurons, such as endocrine and sensory cells, are very similar to those of neurons. Taste receptor cells, which are typical sensory paraneurons, express certain neuron-specific proteins (Huang and Lu, 1996b;Iwanaga et al, 1992;Kanazawa and Yoshie, 1996;Yee et al, 2001). Yoshie et al (1991) demonstrated that certain guinea pig taste bud cells are immunoreactive for spot 35 protein, a protein found in cerebellar Purkinje cells, and that the ultrastructural features of these cells resemble those of type III cells and gave the evidence that paraneurons might share neuron-specific substances with neurons.…”

Section: Introductionmentioning

confidence: 99%

“…

ABSTRACTOn the basis of our previous report that protein gene product 9.5 (PGP 9.5)-immunoreactive nerve fibers and taste cells and calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers are found in guinea pig vallate papillae [Huang and Lu (1996b) . We speculated that PGP 9.5 might be a marker for taste receptor cells and that CGRP might play an important role in taste transmission.

…”

mentioning

confidence: 99%

Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene‐related peptide in vallate taste cells and related nerves in the guinea pig

Huang

2003

Microscopy Res & Technique

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On the basis of our previous report that protein gene product 9.5 (PGP 9.5)-immunoreactive nerve fibers and taste cells and calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers are found in guinea pig vallate papillae [Huang and Lu (1996b) Arch. Histol. Cytol. 59:433-441]. We speculated that PGP 9.5 might be a marker for taste receptor cells and that CGRP might play an important role in taste transmission. We, therefore, performed an immunohistochemical and ultrastructural analysis of taste cells and related nerves in guinea pig vallate papillae. In the connective tissue of the vallate papilla, the ultrastructural data revealed that the PGP 9.5-immunoreactive nerve fibers were both myelinated and unmyelinated. The CGRP-immunoreactive nerve fibers were unmyelinated and surrounded by the cytoplasm of Schwann cells as were the non-immunoreactive fibers. In the vallate taste buds, only type III cells, which make synaptic contacts with intragemmal nerves, were PGP 9.5-immunoreactive, while the nerve terminals making synaptic contact with the underlying type III cells were CGRP-immunoreactive. From these observations, we conclude that: (1) PGP 9.5 might be a useful specific marker for type III cells in guinea pig vallate taste buds; and (2) CGRP-containing nerve fibers might be primarily involved in the neural transmission of taste stimuli.

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Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

Cited by 11 publications

References 29 publications

Neurochemical characterization of sea lamprey taste buds and afferent gustatory fibers: Presence of serotonin, calretinin, and CGRP immunoreactivity in taste bud bi‐ciliated cells of the earliest vertebrates

Neurochemical characterization of sea lamprey taste buds and afferent gustatory fibers: Presence of serotonin, calretinin, and CGRP immunoreactivity in taste bud bi‐ciliated cells of the earliest vertebrates

Innervation of papilla vallata in diabetic rats

Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene‐related peptide in vallate taste cells and related nerves in the guinea pig

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