Immunohistochemical Studies of the Adult Human Ovary: Possible Contribution of Immune and Epithelial Factors to Folliculogenesis

Bukovský, Antonín; Keenan, Jeffrey; Caudle, Michael R.; Wimalasena, Jay; Upadhyaya, Nirmala B.; Meter, Stuart E. Van

doi:10.1111/j.1600-0897.1995.tb00901.x

Cited by 77 publications

(174 citation statements)

References 35 publications

(8 reference statements)

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“…Former observations indicating that neo-oogenesis occurs in adult human and mouse ovaries (Bukovsky et al, 1995(Bukovsky et al, , 2004Johnson et al, 2004;Kerr et al, 2006;Bukovsky et al, 2008a) were recently confirmed by an evidence that cultured OSC transplanted into ovaries of sterilized mice could produce new oocytes and offsprings (Zou et al, 2009). It has been indicated that these observations support the possibility to restore fertility in women with a premature ovarian failure (POF; Gougeon, 2010).…”

mentioning

confidence: 55%

“…The OSC-derived cords are in contact or penetrated by nerve terminals, and fragmented cords (nests) are suggested to contribute epithelial elements to ovarian follicles . Our observations (Bukovsky et al, 1995(Bukovsky et al, , 2004 indicate that epithelial nests lying in proximity to primordial follicles contribute granulosa cells to the follicular renewal during adulthood.…”

Section: Osc Derived Epithelial Crypts (Alternative Germ Cell Precursmentioning

confidence: 85%

“…Germ cells are easily distinguished from smaller coelomic epithelial OSC by their rounded contour, smooth surface, and in some instances, large amoeboid evaginations (Motta and Makabe, 1986). Using differential interference contrast and immunohistochemistry, we previously reported the occurrence of similar putative germ cells within the OSC layer and cortex of adult human ovaries (Bukovsky et al, 1995). These data indicate that germ cells are present in the OSC layer.…”

Section: Identification Of Germ Cells In the Osc Layermentioning

confidence: 85%

“…We proposed that follicular renewal exists during the prime reproductive period (PRP; between menarche and 38 AE 2 years of age in human females) throughout the animal kingdom [PRP doctrine (Bukovsky and Virant-Klun, 2006)]. Both, the granulosa cells and oocytes required for follicular renewal in adult human ovaries originate from OSC (Bukovsky et al, 1995;Bukovsky et al, 2004).…”

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confidence: 99%

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Ovarian Stem Cell Niche and Follicular Renewal in Mammals

Bukovský

2011

The Anatomical Record

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Stem cell niche consists of perivascular compartment, which connects the stem cells to the immune and vascular systems. During embryonic period, extragonadal primordial germ cells colonize coelomic epithelium of developing gonads. Subsequently, ovarian stem cells (OSC) produce secondary germ cells under the influence of OSC niche, including immune system-related cells and hormonal signaling. The OSC in fetal and adult human ovaries serve as a source of germ and granulosa cells. Lack of either granulosa or germ cell niche will result in premature ovarian failure in spite of the presence of OSC. During perinatal period, the OSC transdifferentiate into fibroblast-like cells forming the ovarian tunica albuginea resistant to environmental threats. They represent mesenchymal precursors of epithelial OSC during adulthood. The follicular renewal during the prime reproductive period (PRP) ensures that there are fresh eggs available for a healthy progeny. End of PRP is followed by exponentially growing fetal genetic abnormalities. The OSC are present in adult, aging, and postmenopausal ovaries, and differentiate in vitro into new oocytes. During in vitro development of large isolated oocytes reaching 200 lm in diameter, an ancestral mechanism of premeiotic nurse cells, which operates during oogenesis in developing ovaries from invertebrates to mammalian species, is utilized. In vitro developed eggs could be used for autologous IVF treatment of premature ovarian failure. Such eggs are also capable to produce parthenogenetic embryos like some cultured follicular oocytes. The parthenotes produce embryonic stem cells derived from inner cell mass, and these cells can serve as autologous pluripotent stem cells. Anat Rec, 294:1284Rec, 294: -1306Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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mentioning

confidence: 55%

Section: Osc Derived Epithelial Crypts (Alternative Germ Cell Precursmentioning

confidence: 85%

Section: Identification Of Germ Cells In the Osc Layermentioning

confidence: 85%

mentioning

confidence: 99%

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Ovarian Stem Cell Niche and Follicular Renewal in Mammals

Bukovský

2011

The Anatomical Record

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“…Adult ovaries of Drosophila contain germline stem cells that renew oocyte supply (Lin & Spradling 1995, Deng & Lin 2001, Wang & Lin 2004. In adult human ovaries, surface epithelial cells derived from the tunica albuginea, are reported to differentiate into granulosa cells and oocytes forming new follicles (Bukovsky et al 1995, Bukovsky 2005. Mitotically active cells in the ovarian surface epithelium (OSE) of juvenile and adult mice were suggested by Johnson et al (2004) to be a source of germline stem cells capable of regenerating the population of primordial follicles.…”

Section: Introductionmentioning

confidence: 99%

Quantification of healthy follicles in the neonatal and adult mouse ovary: evidence for maintenance of primordial follicle supply

Kerr¹,

Duckett²,

Myers³

et al. 2006

Reproduction

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Proliferation and partial meiotic maturation of germ cells in fetal ovaries is believed to establish a finite, non-renewable pool of primordial follicles at birth. The supply of primordial follicles in postnatal life should be depleted during folliculogenesis, either undergoing atresia or surviving to ovulation. Recent studies of mouse ovaries propose that intra-and extraovarian germline stem cells replenish oocytes and form new primordial follicles. We quantified all healthy follicles in C57BL/6 mouse ovaries from day 1 to 200 using unbiased stereological methods, immunolabelling of oocyte meiosis (germ cell nuclear antigen (GCNA)) and ovarian cell proliferation (proliferating cell nuclear antigen (PCNA)) and electronmicroscopy. Day 1 ovaries contained 7924G1564 (S.E.M.) oocytes or primordial follicles, declining on day 7 to 1987G203, with 200-800 oocytes ejected from individual ovaries on that day and day 12. Discarded oocytes and those subjacent to the surface epithelium were GCNA-positive indicating their incomplete meiotic maturation. From day 7 to 100 mean numbers of primordial follicles per ovary were not significantly depleted but declined at 200 days to 254G71. Mean numbers of all healthy follicles per ovary were not significantly different from day 7 to 100 (range 2332G349-3007G322). Primordial follicle oocytes were PCNA-negative. Occasional unidentified cells were PCNA-positive with mitotic figures observed in the cortex of day 1 and 12 ovaries. Although we found no evidence for ovarian germline stem cells, our data support the hypothesis of postnatal follicle renewal in postnatal and adult ovaries of C57BL/6 mice. Reproduction (2006) 132 95-109

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