Quantification of healthy follicles in the neonatal and adult mouse ovary: evidence for maintenance of primordial follicle supply

Abstract

Proliferation and partial meiotic maturation of germ cells in fetal ovaries is believed to establish a finite, non-renewable pool of primordial follicles at birth. The supply of primordial follicles in postnatal life should be depleted during folliculogenesis, either undergoing atresia or surviving to ovulation. Recent studies of mouse ovaries propose that intra-and extraovarian germline stem cells replenish oocytes and form new primordial follicles. We quantified all healthy follicles in C57BL/6 mouse ovaries from day 1 to 200 using unbiased stereological methods, immunolabelling of oocyte meiosis (germ cell nuclear antigen (GCNA)) and ovarian cell proliferation (proliferating cell nuclear antigen (PCNA)) and electronmicroscopy. Day 1 ovaries contained 7924G1564 (S.E.M.) oocytes or primordial follicles, declining on day 7 to 1987G203, with 200-800 oocytes ejected from individual ovaries on that day and day 12. Discarded oocytes and those subjacent to the surface epithelium were GCNA-positive indicating their incomplete meiotic maturation. From day 7 to 100 mean numbers of primordial follicles per ovary were not significantly depleted but declined at 200 days to 254G71. Mean numbers of all healthy follicles per ovary were not significantly different from day 7 to 100 (range 2332G349-3007G322). Primordial follicle oocytes were PCNA-negative. Occasional unidentified cells were PCNA-positive with mitotic figures observed in the cortex of day 1 and 12 ovaries. Although we found no evidence for ovarian germline stem cells, our data support the hypothesis of postnatal follicle renewal in postnatal and adult ovaries of C57BL/6 mice. Reproduction (2006) 132 95-109

Redirecting intracellular trafficking and the secretion pattern of FSH dramatically enhances ovarian function in mice

“…Tissues were formalin-or Bouin reagent-fixed, processed, and stained with periodic acid-Schiff reagent/hematoxylin (19). Testis tubule and ovarian follicle counts were calculated as described previously (14,44).…”

The primordial follicle reserve is not renewed after chemical or γ-irradiation mediated depletion

“…We have shown that the size of the primordial follicle reserve pool remains stable in adult C57BL/6 mice between the ages of 25 and 100 days (Kerr et al 2006). This plateau in numbers may indicate a very slow rate of primordial follicle recruitment, and limited loss of primordial follicles through death, such that a decline cannot be detected with the number of animals used or the statistical analysis applied (Faddy & Gosden 2007).…”

Born or made? Debate on mouse eggs reignites

“…At least, "until someone comes up with some killer experiments to prove that bone marrow, or the ovary, is, or is not, the source of new oocytes". Kerr, Jock Findlay and colleagues add a piece of support for regeneration in research to be published online later this month 4 .…”