Immunohistochemical Localization of Manserin, a Novel Neuropeptide Derived from Secretogranin II, in Rat Adrenal Gland, and its Upregulation by Physical Stress

Kamada, Naoki; Tano, Kaori; Oyabu, Akiko; Imura, Yoshio; Narita, Naoko; Tashiro, Yasura; Uchida, Akio; Komada, Yoshihiro; Narita, Minoru

doi:10.1007/s10989-010-9203-2

Cited by 11 publications

(6 citation statements)

References 19 publications

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“…In our study, since manserin was localized in the synapses of the inner and outer hair cells of the organ of Corti, it is suggested that manserin plays a role in the auditory nervous transmission in the same manner as the other neuropeptides, such as dynorphin and enkephalin. We have also found that the upregulation of manserin in response to physical stress (Kamada et al 2010), indicating that manserin is involved in stress response. Therefore, manserin might be involved in the occurrence of stress-related inner ear diseases, such as sudden deafness and Ménière disease.…”

Section: Discussionsupporting

confidence: 54%

“…Affinity-purified rabbit anti-manserin antibody was prepared as described previously (Yajima et al 2004;Kamada et al 2010;Tano et al 2010). The anti-synaptophysin antibody (Chemicon, Temecula, CA), anti-peripherin antibody (Santa Cruz biotechnology, Santa Cruz, CA), anti-myosin VIIa antibody (Santa Cruz biotechnology), and anti-neuronal class III β-tubulin antibody TUJ1 (Covance, Richmond, CA) were purchased.…”

Section: Antibodiesmentioning

confidence: 99%

“…Information of these peptides being localized in hypothalamic nuclei and pituitary and adrenal glands led us to speculate that these peptides may function as a neurotransmitter in the endocrine and neuronal system, although their receptors have not been identified yet. We recently reported that manserin's expression in the adrenal is stress dependent (Kamada et al 2010), indicating that manserin might be involved in stress response. There are many stress-related diseases in modern society.…”

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confidence: 99%

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Existence of Manserin, a Secretogranin II–Derived Neuropeptide, in the Rat Inner Ear

Ida-Eto

Oyabu

Ohkawara

et al. 2011

J Histochem Cytochem.

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Manserin is a 40-amino acid neuropeptide derived from rat brain. Manserin has been shown to distribute in the neuroendocrine system, such as the pituitary and adrenal glands, but it has been little studied in other organs. In this study, the authors examined localization of manserin in the inner ear of the adult Wistar rat using immunohistochemical analyses. Manserin immunoreactivity was detected in the neuronal terminals of the organ of Corti and type II spiral ganglion cells. In addition to being identified in the auditory system, manserin was detected at the synapses of the vestibular system, such as saccule, utricle, and semicircular canal. These results suggest that inner ear manserin may be involved in the function of peripheral auditory and vestibular systems.

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Section: Discussionsupporting

confidence: 54%

Section: Antibodiesmentioning

confidence: 99%

mentioning

confidence: 99%

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Existence of Manserin, a Secretogranin II–Derived Neuropeptide, in the Rat Inner Ear

Ida-Eto

Oyabu

Ohkawara

et al. 2011

J Histochem Cytochem.

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“…Anti-manserin antibody was prepared as described previously (Yajima et al 2004;Kamada et al 2010). The specificity of anti-manserin antibody has been previously confirmed by immunoblotting (Yajima et al 2004) and the fact that the reactions were specifically inhibited in the absence of the primary antibody (data not shown).…”

Section: Antibodiesmentioning

confidence: 91%

“…Manserin also occurred in the upper villi of the duodenum, colocalized with TUNEL-positive cells (Yajima et al 2008), suggesting its role in the mechanism of apoptosis. Very recently, we found that manserin, expressing in the rat adrenal medulla, was upregulated in response to physical restraint stress (Kamada et al 2010), suggesting its role in stress response. However, physiological roles of manserin are still unsettled.…”

Section: Introductionmentioning

confidence: 94%

Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Tano

Oyabu

Tashiro

et al. 2010

Histochem Cell Biol

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Manserin is a recently characterized 40-amino acid neuropeptide derived from secretogranin II, a protein belonging to the chromogranin family. Although the physiological roles of manserin have not been elucidated to date, manserin has been shown to distribute in not only the brain but also the endocrine system such as the pituitary and adrenal glands, suggesting its role in the endocrine system. The present study aimed to explore the occurrence and distribution of manserin in the rat pancreas using an immunohistochemical technique with a polyclonal antibody against rat manserin. Immunoreactivity for manserin was readily detected in almost whole islets of Langerhans whereas not at all in the exocrine pancreas. Manserin-expressing cells were not colocalized with the glucagon-secreting cells (alpha cells), whereas they colocalized with insulin-secreting cells (beta cells) and somatostatin-secreting cells (delta cells), although their intracellular distribution was different. These results indicate that manserin, occurring in the endocrine pancreas, may have a potential role in the endocrine system.

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Secretogranin II and its Derivative Peptide, Manserin, are Differentially Localized in Purkinje Cells and Unipolar Brush Cells in the Rat Cerebellum

Ohkawara

Oyabu

Ida-Eto

et al. 2011

Int J Pept Res Ther

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Immunohistochemical Localization of Manserin, a Novel Neuropeptide Derived from Secretogranin II, in Rat Adrenal Gland, and its Upregulation by Physical Stress

Cited by 11 publications

References 19 publications

Existence of Manserin, a Secretogranin II–Derived Neuropeptide, in the Rat Inner Ear

Existence of Manserin, a Secretogranin II–Derived Neuropeptide, in the Rat Inner Ear

Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Secretogranin II and its Derivative Peptide, Manserin, are Differentially Localized in Purkinje Cells and Unipolar Brush Cells in the Rat Cerebellum

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