Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Abstract

Manserin is a recently characterized 40-amino acid neuropeptide derived from secretogranin II, a protein belonging to the chromogranin family. Although the physiological roles of manserin have not been elucidated to date, manserin has been shown to distribute in not only the brain but also the endocrine system such as the pituitary and adrenal glands, suggesting its role in the endocrine system. The present study aimed to explore the occurrence and distribution of manserin in the rat pancreas using an immunohistochemical technique with a polyclonal antibody against rat manserin. Immunoreactivity for manserin was readily detected in almost whole islets of Langerhans whereas not at all in the exocrine pancreas. Manserin-expressing cells were not colocalized with the glucagon-secreting cells (alpha cells), whereas they colocalized with insulin-secreting cells (beta cells) and somatostatin-secreting cells (delta cells), although their intracellular distribution was different. These results indicate that manserin, occurring in the endocrine pancreas, may have a potential role in the endocrine system.

Histochemistry and cell biology: the annual review 2010

“…So far the physiological relevance of manserin is still enigmatic. However, as Sg II-related peptides have been proven to be useful as possible tumour markers, Tano et al (2010) see a potential of manserin as a diagnostic and prognostic marker for endocrine tumours (Conlon 2009). Gouyer et al (2010) performed a thorough characterization of the mucin MUC6 in wild-type and also in Cftr-deWcient mice.…”

“…Research on manserin was the focus of Tano et al (2010). Manserin, derived from precursor molecule secretogranin II (SgII), belongs to a group of acidic, secretory proteins of endocrine, neuroendocrine and neuronal cells, which are referred to as chromogranins, secretogranins or granins.…”

Newly Identified Regulators of Ovarian Folliculogenesis and Ovulation

“…Almost 90% of SCG2 is cleaved, generating three bioactive peptides, secretoneurin (SN), EM66, and manserin [ 96 , 97 ]. Although SN has been shown to be involved in many processes, including LH secretion and angiogenesis [ 97 ], the role of the other peptides is not fully understood as yet [ 98 ]. A recent study found that hCG induced upregulation in SCG2 mRNA levels in human, macaque monkey and rodents ovaries [ 99 ].…”

Differential expression and processing of secretogranin II in relation to the status of pheochromocytoma: implications for the production of the tumoral marker EM66

“…In particular, the sequence of human secretogranin II (SgII or SCG2 as given in the HUGO database) contains nine pairs of basic amino acids that represent potential cleavage sites by PCs. Indeed, proteolytic processing of SgII leads to the production of several peptides, such as secretoneurin (SN; SgII 152-184 ) which regulates neurotransmission, inflammatory responses, and gonadotrope activity (Fischer-Colbrie et al 2005, Shyu et al 2008, Zhao et al 2010; EM66 (SgII ) which may participate in the control of feeding behavior in rodents (Boutahricht et al 2005(Boutahricht et al , 2007; and manserin ) whose putative role remains to be established (Yajima et al 2004, Tano et al 2010. The ubiquitous distribution of granins and their derived peptides in nervous, endocrine, and neuroendocrine tissues makes these entities useful markers of secretion from neuroendocrine cells and neoplasms (Rosa & Gerdes 1994).…”