Immunohistochemical localization of intermediate filament proteins in calcifying epithelial odontogenic tumors

Abstract

Three calcifying epithelial odontogenic tumors (CEOT) were examined immunohistochemically. The localization of intermediate filaments was characterized through the use of polyclonal anti‐keratin antiserum (TK which detects 41–65 kd keratins), 2 monoclonal keratin antibodies (PKK1 specific for the 44, 46, 52 & 53 kd keratins and KL1, specific for the 55–57 kd keratins) and monoclonal antibodies for vimentin and desmin. The tumor epithelial cells were slightly positive or negative for PKK1 detectable keratins, but slightly to strongly positive for KL1 and TK antibodies. Tumor epithelium was slightly positive for vimentin but negative for desmin. The tumor foci were composed of both dark‐staining and pale‐staining cells; the former had a more intense reaction with KL1 and TK antibodies than the latter. Homogeneous acellular material was either PAS‐positive or negative, with or without calcification, and keratin‐negative.

Calcifying epithelial odontogenic tumor: an immunohistochemical case study

“…Consistent with many previous studies reporting the negativity of the amyloid-like material to various different molecular weight cytokeratins (Mori et al 1988;Asano et al 1990;Saku et al 1992;Takata et al 1993;Kumamoto et al 1999Kumamoto et al , 2001Cheng et al 2002;Crivelini et al 2003), the present study found that the amyloid-like deposits did not react to cytokeratins that are present in the antibody clone AE1/AE3. Nevertheless, one study showed that the amyloid deposit is immunoreactive for the cocktail of cytokeratins 1, 5, 6, 8, 13, 16 and pan-cytokeratins AE1/AE3 (Aviel-Ronen et al 2000).…”

“…Many previous studies have shown the immunoreactivity of CEOT-neoplastic cells to many cytokeratins including cytokeratins 14, 19, pan-cytokeratins AE1/ AE3 and a cocktail of cytokeratins 1, 5, 6, 8, 18, 19 (Sauk et al 1985;Mori et al 1988;Asano et al 1990;Saku et al 1992;Takata et al 1993;Aviel-Ronen et al 2000;Kumamoto et al 2001;Crivelini et al 2003). These results indicate the epithelial nature of the CEOT-neoplastic cells.…”

“…Approximately 12 cases of CEOTs in four articles have involved immunohistochemical studies with antivimentin antibody (Sauk et al 1985;Mori et al 1988;Takata et al 1993;Crivelini et al 2003). Of these, seven cases showed positive reaction to vimentin.…”

“…There have been less than 40 documented cases of CEOTs that were immunohistochemically investigated with a variety of antibodies (Sauk et al 1985;Mori et al 1988;Asano et al 1990;Saku et al 1992;Takata et al 1993;Aviel-Ronen et al 2000;Kumamoto et al 2001;Crivelini et al 2003;Solomon et al 2003). Among these findings, the nature of the amyloid-like materials remains controversial partly due to the small number of CEOT cases examined.…”

Calcifying Epithelial Odontogenic Tumor (Ceot)

“…In a study examination of CEOT immunohistochemically for localization of intermediate filament proteins, the tumor epithelium cells were slightly positive or negative for (monoclonal keratin bodies) PKKI detectable keratins, but slightly to strong positive for KLI (monoclonal keratin bodies) and TK (polyclonal anti-keratin antibodies), tumor epithelium was slightly positive for vimentin but negative for desmin 32 . Two enamel proteins, amelogenin and enamelin were located in small foci in a case of CEOT when detected immunohistochemically 33 .…”