Calcifying odontogenic cyst immunohistochemical detection of keratin and involucrin in cyst wall

Abstract

Calcifying odontogenic cysts (COC) were immunohistochemically described using different keratin proteins and involucrin as well as histopathology. The cystic lining epithelium was composed of calcifying, keratinizing, squamous, and columnar epithelial cells, and included calcified masses of irregular shape and various size as well as ghost cells. Calcifying epithelium gave negative or only trace staining for keratins detected with low molecular keratin (PKK1), but were regularly positive with high molecular keratin (KL1) and polyclonal antibody for keratin (TK). They were occasionally positive for involucrin. The cells located in the periphery of the calcified masses had a particular abundance of high molecular weight and total keratins (KL1 and TK). Calcified bodies and ghost cells were devoid of any immunoreactivity. Squamous epithelium was relatively similar to that of normal squamous cell epithelium in the oral mucosa. It were most commonly found in columnar cystic epithelial cells which displayed intense staining with all immunoreagents. It is postulated that such epithelial cells may have a strong potentiality to transform into ghost cells or to undergo metaplasia. They may develop altered synthesis of homogenous acellular materials and finally become transformed into calcifying epithelium containing dystrophic calcified masses.

Calcifying odontogenic cyst: clinicopathological features and immunohistochemical profile of 10 cases

“…Dentinoid material can be found adjacent to the epithelial/connective tissue junction. Cytokeratins of the ghost cells can suffer dystrophic calcification, which is also variable from lesion to lesion (7, 17). Odontoma‐associated COC seems to show a greater amount of luminal and mural dentinoid substance and also more luminal ghost cells than COCs not associated with odontomas (12).…”

“…Cytokeratin expression in COC is frequently demonstrated in cells of the epithelium lining, but it is controversial in the ghost cells (2, 17, 22). This antigenic alteration is probably due to coagulative necrosis of the odontogenic epithelium in COC (2).…”

“…This antigenic alteration is probably due to coagulative necrosis of the odontogenic epithelium in COC (2). Expression of high‐ and low‐molecular weight cytokeratins in the epithelium lining of COC and its weak positivity or negativity in ghost cells suggest that COC is an aberrant counterpart of the normal dental development (7, 17, 19, 22). Cytokeratin 19 is expressed in all odontogenic epithelia from dental germs in development and in some odontogenic tumors, including COC (8, 23, 24) and, as expected, was expressed in our 10 cases.…”

Ameloblastic fibroma: growth potentiality of odontogenic epithelium and coexpression of intermediate filament proteins in fibromatous cells

“…eal findings of epithelial cell proliferations. These distribution patterns are 'compared with those of dental lamina, tooth germs and odontogenic epithelial lesions previously reported (6)(7)(8)(9)(10)(11)(12)(13).…”

“…There have been several reports of an immunohistochemical identification of keratins in developing tooth germs (6,7), odontogenie tumors; ameloblastomas (8-10), squamous odontogenie tumors (11), blastomie fibromas have yet been calcifying epithelial odontogenie tu-made. The present report describes the mors (12), and ealeifying odontogenie immunohistochemieal distribution of cysts (13). However, no immunohisto-intermediate filament proteins in amechemieal studies of keratins in amelo-loblastie fibromas and histopathologi-Tablc 1.…”