Immunohistochemical localization of fibronectin-like protein in the inner ear of the developing gerbil and rat

Woolf, Nigel K.; Koehrn, Fred J.; Ryan, Allen F.

doi:10.1016/0165-3806(92)90004-g

Cited by 39 publications

(26 citation statements)

References 49 publications

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“…An understanding of the mechanisms by which these afferent connections are constructed will require additional study. However, it is of interest to note that fibronectin-like protein, a substrate adhesion molecule known to be important in neurite guidance (34), is transiently expressed within the extracellular spaces below gerbil IHCs and OHCs from birth to P4 (35), the time period during which these nonspecific afferent arbors are forming.…”

Section: Discussionmentioning

confidence: 99%

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Echteler

1992

Proc. Natl. Acad. Sci. U.S.A.

120

152

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The ammalian ear contains two types of auditory receptors, inner and outer hair cells, that lie in dose proximity to each other within the sensory epithelium of the cochlea. In adult mammals, these two classes of auditory hair cells are innervated by separate populations ofafferent neurons that differ strikingly in their cellular morphology and their pattern of arborization within the cochlea. At present, it Is unclear when or how these disinctive patterns of cochlear innervation emerge and become segregated during development. In the present study, an in vitro horseradish peroxidase labeling method was used to examine the formation of individual auditory neuron arbors at the same location within the apex of the developing gerbil cochlea. At birth, most cochlear neurons displayed peripheral arbors that embraced both inner and outer hair cell receptors. During the next 6 days, however, the arbors of individual cochlear afferents become confined to either the inner or outer hair cell zone, and thus there is a complete segregation of afferent innervation. This neural segregation occurs principally through the withdrawal of inappropriate connectimns to the outer hair cell ystem and is completed well before hearing commences.A central goal of neurobiology is to determine how developing neurons choose their specific synaptic partners. An excellent system for examining this issue is the mammalian auditory endorgan, the cochlea, since its pattern of innervation is among the most precise of any sensory or motor system. The adult cochlea contains two types of auditory receptors, termed inner (IHCs) and outer (OHCs) hair cells, which receive separate and highly distinctive patterns of afferent innervation. Ninety percent of all cochlear afferents project to individual IHCs via thick unbranched radial afferents, each of which terminates in a single synapse. The OHCs, on the other hand, receive a more diffuse innervation from a relatively few thin spiral afferents, each of which projects for hundreds of micrometers along the length of cochlea before branching to contact from 5 to 50 receptorsUltrastructural studies have provided evidence for substantial ontogenic changes in the form, number, and spatial arrangement of synapses between mammalian auditory receptors and cochlear nerve fibers (9-16). To understand the process by which these synaptic patterns are adjusted during development, however, it is necessary to examine the early morphological changes that occur in the formation of indi- In the present investigation, I have employed an in vitro horseradish peroxidase (HRP) method that permits labeling of small populations of auditory nerve fibers at any location within the developing cochlea. The Mongolian gerbil was selected for study since this species displays a prolonged period of postnatal cochlear maturation. In addition, the gerbil cochlea, like that of the mouse (26), lengthens <10% after birth, from an average of 10.8 mm in the newborn (unpublished data) to 12.1 mm in the adult (27), which greatly reduces ...

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Section: Discussionmentioning

confidence: 99%

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Echteler

1992

Proc. Natl. Acad. Sci. U.S.A.

120

152

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“…In the rat, gerbil, and mouse, fibronectin is abundant in the basilar membrane but is largely absent from around cells in the organ of Corti (Woolf et al, 1992;Cosgrove and Rodgers, 1997). However, during a short interval of a few days, the protein accumulates in the synaptic region beneath both types of hair cells (Woolf et al, 1992). Fibronectin and laminin exist in the spiral ganglion of the mouse during development (Woolf et al, 1992;Cosgrove and Rodgers, 1997).…”

mentioning

confidence: 98%

“…In the bird hearing organ, the basilar papilla, neither fibronectin nor laminin is detected along the growth routes of nerve fibers, although fibronectin exists beneath the hyaline cells (Richardson et al, 1987;Hemond and Morest, 1991;Cotanche and Messana, 1996). In the rat, gerbil, and mouse, fibronectin is abundant in the basilar membrane but is largely absent from around cells in the organ of Corti (Woolf et al, 1992;Cosgrove and Rodgers, 1997). However, during a short interval of a few days, the protein accumulates in the synaptic region beneath both types of hair cells (Woolf et al, 1992).…”

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confidence: 98%

Tenascin-C in the cochlea of the developing mouse

Whitlon

Zhang

Kusakabe³

1999

J. Comp. Neurol.

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“…With respect to the other ECM candidates, strong expression of FN has been documented immediately beneath the inner and outer HCs, in the rat cochlea from E18 through P1 [Woolf et al, 1992]. Kim [pers.…”

Section: Discussionmentioning

confidence: 99%

“…Besides these diffusable molecules, the inner ear tissue of the developing mammal contains a dynamic arrangement of insoluble glycoproteins such as laminin-1 (LN), tenascin-C (TN) and fibronectin (FN) in the extracellular matrix (ECM). The temporal and spatial expression patterns of ECM molecules have been suggested to influence reactions such as differentiation, morphogenesis, process outgrowth and substrate attachment by interacting with receptors on the surfaces of cochlear neurons [Woolf et al, 1992;Cosgrove and Rodgers, 1997;Whitlon et al, 1999a, b], but the influence of LN and TN on the outgrowth of SG neurites has not been investigated to date.…”

Section: Introductionmentioning

confidence: 99%

The Disintegrin Kistrin Inhibits Neurite Extension from Spiral Ganglion Explants Cultured on Laminin

Aletsee

Mullen²,

Kim³

et al. 2001

Audiol Neurotol

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The influence of laminin-1 (LN) and tenascin-C (TN), extracellular matrix molecules expressed spatially and temporally along the neural growth route from spiral ganglion (SG) neurons to the cochlear sensory cells, was evaluated in cultured SG explants from postnatal day 4 rats. Increasing concentrations of LN resulted in a strong, dose-dependent increase in the length of neurites and in a higher number of neural processes, while varying TN concentrations had relatively minor effects on both parameters. The results suggest differential receptor activation by LN and TN. When explants grown on LN were treated with Kistrin, an inhibitor of the αvβ3 integrin, the LN-induced increase in neurite length was reduced in a dose-dependent manner. However, the number of extending neurites was not affected, indicating that different receptors mediate this response, perhaps by increasing neuronal survival.

show abstract

Immunohistochemical localization of fibronectin-like protein in the inner ear of the developing gerbil and rat

Cited by 39 publications

References 49 publications

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Developmental segregation in the afferent projections to mammalian auditory hair cells.

Tenascin-C in the cochlea of the developing mouse

The Disintegrin Kistrin Inhibits Neurite Extension from Spiral Ganglion Explants Cultured on Laminin

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