Tenascin-C in the cochlea of the developing mouse

Whitlon, Donna S.; Zhang, Xueli; Kusakabe, Moriaki

doi:10.1002/(sici)1096-9861(19990412)406:3<361::aid-cne5>3.0.co;2-o

Cited by 28 publications

(20 citation statements)

References 94 publications

(132 reference statements)

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“…Fig 9D, shows laminin-β2-IR in human kidney BMs as has been previously demonstrated (Virtanen et al, 1995). Tenascin-C-IR in the organ of Corti of mouse (Fig 9E) is similar to previously published (Whitlon et al, 1999). Figure 9F shows AQP4 immunoreactivity in Hensen cells of the human organ of Corti as previously reported (Lopez et al, 2007).…”

Section: Resultssupporting

confidence: 90%

“…There are only a few such studies of the vestibular periphery in any mammalian model (Yamashita et al, 1991; Swartz and Santi, 1999), and there are less than a handful of studies that investigate the composition of human cochlear BMs (Zehnder et al, 2005; Yamashita et al, 1991; Kleppel et al, 1989). And while the expression of laminin and collagen II in the human fetal inner ear has been studied (Yamashita et al, 1991; Khetarpal et al, 1994), developmentally-dependent patterns of expression of ECM molecules have been well-documented (Rodgers et al, 2001; Yamashita and Sekitani, 1992; Whitlon et al, 1999). This validates studying the distribution in the adult human.…”

Section: Introductionmentioning

confidence: 99%

“…There are no previous studies in the adult human inner ear examining collagen IVα2, nidogen, or laminin-β2 expression. There are even fewer studies examining the expression of the ECM proteins, α-dystroglycan and tenascin-C. All of the prior studies have been in rodents: mouse (Heaney and Schulte, 2003; Whitlon et al, 1999); chinchilla (Swartz and Santi, 1999; Tsuprun and Santi, 1999) and gerbil (Heaney et al, 2002). There are no prior studies of these ECM macromolecules in the human cochlear or vestibular system, and only one prior study of the vestibular system (Swartz and Santi, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Tenascin-C has been implicated in several developmental processes, most notably neural morphogenesis (Wehrle and Chiquet, 1990; Warchol and Speck, 2007), wound healing (Alford and Hankenson, 2006), and tumorigenesis (reviewed by Orend, 2005). The expression and distribution of tenascin-C in the cochlea and vestibular endorgans of chinchilla (Swartz and Santi, 1999; Tsuprun and Santi, 1999) and the developing mouse (Whitlon et al, 1999) has been studied. To our knowledge, there are no prior studies of tenascin-C expression in the auditory or vestibular endorgan in human.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects

et al. 2009

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The immunolocalization of several basement membrane (BM) proteins was investigated in vestibular endorgans microdissected from temporal bones obtained from subjects with a documented normal auditory and vestibular function (n = 5, average age = 88 years old). Fluorescent immunostaining using antibodies directed at collagen IVα2, nidogen-1, laminin-β2, α-dystroglycan, and tenascin-C was applied to cryosections from human cochlea, cristae ampullares, utricular and saccular maculae. Collagen IVα2, nidogen-1, and laminin-β2 localized to all subepithelial cochlear BMs, Reissner's membrane, strial and spiral ligamental perineural and perivascular BMs, and the spiral limbus. Tenascin-C localized to the basilar membrane and the osseous spiral lamina. α-dystroglycan localized to most cochlear BMs except those in the spiral ligament, basilar membrane and spiral limbus. Collagen IV, nidogen-1, and laminin-β2 localized to the subepithelial BMs of the maculae and cristae ampullares, and the perineural and perivascular BMs within the underlying stroma. The BM underlying the transitional and dark cell region of the cristae ampullares also expressed collagen IV, nidogen-1, and laminin β2. Tenascin-C localized to the subepithelial BMs of the utricular maculae and cristae ampullares, and to calyx-like profiles throughout the vestibular epithelium, but not to the perineural and perivascular BMs. α-dystroglycan colocalized with aquaporin-4 in the basal vestibular supporting cell, and was also expressed in the subepithelial BMs, as well as perivascular and perineural BMs. This study provides the first comprehensive immunolocalization of these ECM proteins in the human inner ear. The validity of the rodent models for inner ear disorders secondary to BM pathologies was confirmed as there is a high degree of conservation of expression of these proteins in the human inner ear. This information is critical to begin to unravel the role that BMs may play in human inner ear physiology and audiovestibular pathologies.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects

et al. 2009

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“…Several ECM molecules are positioned along the path of the developing afferent dendrites that link the cell bodies of spiral ganglion neurons (SGNs) to their target cells in the sensory epithelium (Woolf et al 1992; Whitlon et al 1999a, b). Moreover, the neurites of SGNs have been shown to respond strongly to ECM molecules in vitro.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of MMP-2 but not MMP-9 Influences Inner Ear Spiral Ganglion Neurons In Vitro

et al. 2014

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Matrix metalloproteinases (MMPs) play an important role in modeling of the extracellular matrix. There is increasing evidence that these proteases are important in neurite elongation and axonal guidance during development in the central nervous system and retina. Moreover, they are also expressed after acute injury and can be the key mediators of pathogenesis. However, the role of MMPs in the inner ear is largely unknown. Our group recently demonstrated that general inhibition of MMPs resulted in auditory hair cell loss in vitro. In the present study, we investigated the role of MMPs in inner ear spiral ganglion neuron (SGN) survival, neuritogenesis and neurite extension by blocking MMPs known to be involved in axonal guidance, neurite elongation, and apoptosis in other neuronal systems. Spiral ganglion (SG) explants from 5-day-old Wistar rats were treated with different concentrations of the general MMP inhibitor GM6001, a specific MMP-2 inhibitor, and a specific MMP-9 inhibitor, in vitro. The general inhibitor of MMPs and the specific inhibition of MMP-2 significantly reduced both the number of neurites that extended from SG explants, as well as the length of individual neurites. However, neither the general inhibitor of MMPs nor the specific inhibition of MMP-2 influenced SGN survival. Inhibition of MMP-9 had no influence on SGNs. The data suggest that MMPs, and more specifically MMP-2, influence the growth of developing afferent neurites in the mammalian inner ear in vivo.

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Postnatal development of efferent synapses in the rat cochlea

2000

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The development of olivocochlear efferent axons and their contacts in the postnatal cochlea was studied after DiI applications to the olivocochlear bundle in the ipsilateral brainstem of rats from 0 to 10 days of age (P0-10). Light microscopic analyses showed that labeled axons reached the vicinity of inner hair cells by P0 and outer hair cells by P2. Electron microscopic analyses demonstrated that labeled immature efferent axons are present among supporting cells of the greater epithelial ridge as well as inner hair cells at P0. The first efferent contacts that contacted inner hair cells contained a few irregularly sized vesicles and, occasionally, mitochondria. Postsynaptic specializations within inner hair cells apposed to labeled efferent axons included subsynaptic cisterns, irregularly sized vesicles, and synaptic bodies. Similar features were present in unlabeled profiles, presumed to be afferents, indicating that immature efferent axons could not be reliably distinguished from afferents without positive labeling. Efferent axons synapsed with outer hair cells by P4 and had synapse-like contacts at the bases of Deiters' cells at P4 and P6. Contacts between afferents and efferents were observed frequently in the inner spiral bundle from P6. As they matured, efferent axon terminals contacting hair cells contained increasing numbers of synaptic vesicles and were typically apposed by well-defined postsynaptic cisterns, thus acquiring distinctive profiles.

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Tenascin-C in the cochlea of the developing mouse

Cited by 28 publications

References 94 publications

Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects

Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects

Inhibition of MMP-2 but not MMP-9 Influences Inner Ear Spiral Ganglion Neurons In Vitro

Postnatal development of efferent synapses in the rat cochlea

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