Immunohistochemical Localization of Deleted in Malignant Brain Tumors 1 in Normal Human Tissues

Abstract

Deleted in malignant brain tumors 1 (DMBT1) is part of the innate immune system and is expressed on mucosal surfaces in various tissues throughout the human body. However, to date, the localization of DMBT1 has not been investigated systematically and comprehensively in normal human tissues. In this study, we analyzed the mRNA expression of DMBT1 in human tissue by quantitative real-time PCR and examined its localization and distribution in the tissue by immunohistochemical staining using the monoclonal DMBT1 antibody HYB213-6. Anti-ovalbumin was used as an isotype control. The highest level of mRNA expression of DMBT1 was found in the small intestine, and the expression level was high throughout the luminal digestive tract. The expression of DMBT1 was especially high in the luminal digestive tract and salivary glands. The lowest expression level was found in the spleen. Immunohistochemical staining showed a high expression level of DMBT1 on mucosal surfaces throughout the body. There was a clear correlation between the mRNA expression and immunohistochemical expression of DMBT1 in the tissue. DMBT1 is strongly expressed on mucosal surfaces and in salivary glands

Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immunoinhibitory receptor Siglec-8

“…Notably, anti-DMBT1 failed to stain cartilage, but it did stain the same submucosal acini as Siglec-8-Fc (Fig 7, B-F). These data are consistent with the recent report of DMBT1 on human airway submucosal glands and ducts but not respiratory epithelium, 23 as previously reported for Siglec-8 ligands. 9 Likewise, single-cell transcriptomics revealed that DMBT1 gene expression in human lungs and airways is remarkably restricted to submucosal gland cells.…”

“…In nasal lavage samples, most DMBT1, a multifunctional protein, does not carry the Siglec-8 binding determinant, is likely decorated with other glycans, and supports other functions on the airway surface. 18,19 DMBT1 is expressed on many human tissues, 23 most abundantly on intestine and salivary gland, where it may not carry Siglec-8 ligands (preliminary data not shown).…”

Transcriptome Analysis of Pterygium and Pinguecula Reveals Evidence of Genomic Instability Associated with Chronic Inflammation

“…DMBT1 (Deleted in Malignant Brain Tumors 1) and SCGB3A1 (Secretoglobin Family 3A Member 1) encode small secreted proteins expressed at high levels by mucosal tissues throughout the body [ 39 , 40 , 41 ]. An early microarray study also reported downregulation of SCGB2A1 in pterygium [ 18 ].…”

No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis

“…A recent study investigating the distribution and localization of DMBT1 in healthy human tissue found that the DMBT1 cycle threshold (Ct) values in the jejunum and colon were 17 and 22, respectively 36 . In comparison, the Ct values for saline-treated mice were 28 and 20 for the jejunum and colon, respectively.…”