Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immunoinhibitory receptor Siglec-8

Rethinking neutrophils and eosinophils in chronic rhinosinusitis

“…Whether the levels of DMBT1 S8 are altered in various forms of CRS is currently being investigated. 72,73 Finally, it is important to recognize that there are some reports, such as those using antieosinophil pharmacologic approaches, that raise some interesting conundrums regarding the role of eosinophils in CRS. In a sizable study, an oral agent called dexpramipexole, which causes a gradual but marked reduction in eosinophils, was administered to explore its impact on signs and symptoms of CRSwNP.…”

Human brain sialoglycan ligand for CD33, a microglial inhibitory Siglec implicated in Alzheimer’s disease

“…CD33 binds most avidly to this same glycan ( 21 ). In other tissues, this structure is carried on terminally sialylated keratan sulfate chains ( 22 , 23 ). To test whether the human brain CD33 and Siglec-8 ligands were likewise sialylated keratan sulfates, brain protein extract was treated with glycohydrolases ( Fig.…”

“…Prior to electrophoresis, some aliquots of selected samples (as indicated) were dialyzed against PBS and then treated with glycohydrolases as described previously ( 22 ). For each enzyme, control incubations were performed under identical conditions without enzyme.…”

Recent Progress in the Methodologies to Identify Physiological Ligands of Siglecs

“…Also, the affinity purification of integral membrane proteins serving as Siglec ligand requires disruption of the cell membrane by detergent or chaotropic ion, which inevitably dissociates cell surface protein complexes. Many Siglec ligands recently identified via affinity purification are soluble proteins (11,(13)(14)(15), likely because membrane solubilization leads to loss of the cell surface protein complex, which may be a prerequisite for Siglec -ligand interaction.…”