Immunohistochemical localization of an N-acetyl amino-carbohydrate specific lectin (ACL-I) of the marine sponge Axinella corrugata

Dresch, Roger Remy; Zanetti, Gilberto Dolejal; Kanan, João Henrique Corrêa; Mothes, Beatriz; Lerner, Cléa; Trindade, Vera Maria Treis; Henriques, Amélia Teresinha; Vozari-Hampe, Magdolna M.

doi:10.1016/j.acthis.2010.07.005

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Halichondrin B from the Japanese black sponge Halichondria okadai as an effective anticancer drug [28], can modulate microtubule dynamics. Many types of lectins are also produced in various species of sponges, including galectins, N-acetylamino-carbohydrate-specific lectin, c-type lectin, and so on [29,30]. The Aphrocallistes vastus lectin (AVL) is a Ca2+-dependent lectin and inhibited by bird’s nest glycoprotein and D-galactose.…”

Section: Introductionmentioning

confidence: 99%

Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent

Xiang

Liu

et al. 2019

Marine Drugs

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Lectins display a variety of biological functions including insecticidal, antimicrobial, as well as antitumor activities. In this report, a gene encoding Aphrocallistes vastus lectin (AVL), a C-type lectin, was inserted into an oncolytic vaccinia virus vector (oncoVV) to form a recombinant virus oncoVV-AVL, which showed significant in vitro antiproliferative activity in a variety of cancer cell lines. Further investigations revealed that oncoVV-AVL replicated faster than oncoVV significantly in cancer cells. Intracellular signaling elements including NF-κB2, NIK, as well as ERK were determined to be altered by oncoVV-AVL. Virus replication upregulated by AVL was completely dependent on ERK activity. Furthermore, in vivo studies showed that oncoVV-AVL elicited significant antitumor effect in colorectal cancer and liver cancer mouse models. Our study might provide insights into a novel way of the utilization of marine lectin AVL in oncolytic viral therapies.

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Section: Introductionmentioning

confidence: 99%

Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent

Xiang

Liu

et al. 2019

Marine Drugs

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“…Different from this study, it was found that the lectin from sea cucumber had 50% reduced activity in 4 M urea [10] and the lectin from Arisaema tortuosum showed 50% reduced activity in 3 M urea and thiourea and 50% reduced activity in 3.5 M of guanidin-HCl [9]. Therefore, it was identified that although urea gave no effect to the lectins from both mung bean seeds, thiourea and guanidine-HCl affected them.…”

Section: Discussionmentioning

confidence: 63%

Comparison of Biochemical Characterization of Korean and Chinese Mung Bean Lectin

Roh

2014

Journal of Life Science

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The lectins were separated from Korean and Chinese mung bean seeds finally via chromatography using Sephadex G-100 and their biochemical features were studied and compared. They showed no hemagglutination with human red blood cells regardless of trypsin treatment and showed hemagglutination with only trypsin treated rabbit red blood cells. The molecular weights of two lectins were identified as 54 kDa and 28 kDa by SDS-PAGE. It was found that while the optimal reaction temperature of the lectin from Korean mung bean was 60℃, that of the lectin from Chinese mung bean seeds was 50℃. It was found also that the most thermal stable temperature of the seed lectin from Korean mung bean seeds was 50℃ and the lectin from Chinese mung bean was 40-50℃. The lectin from Korean mung bean seeds showed the highest activity at pH 3.2 and the lectin from Chinese mung bean showed the highest activity at pH 6.2. It was identified that when treating a denaturant, thiourea and guanidine-HCl resulted in no hemagglutination, so they induced denaturalization. It was identified also that there was no hemagglutination with urea, so it did not induced denaturalization. They showed no septicity to 6 types of carbohydrates including D-glucose. In addition, the lectins from the two mung bean seed had specificity to metal ions.Key words : Biochemical characterization, Korean and Chinese mung bean seed, lectin *Corresponding author *Tel : +82-53-580-5207, Fax : +82-53-580-5164 *E-mail : rks@kmu.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionMung bean (Phaseolus radiatus L. var.) is an annual plant which belongs to Leguminosae and is originated from India and cultivated also in Korea. Length of its stem is 60~80 cm and brown pili are spread on the whole of plant. Its pod is 5~6 cm in length, has slender and long shape with rough pili and projections, and contains 10-15 fruits. As it is highly nutritious, containing about 60% of starch and 21% of protein as main ingredients, it serves an important nutrition source to animals as well as humans and is also used as a major protein source due to its higher fiber content and lower fat content [28].Lectin is one of natural proteins cohering reversibly to specific monosaccharide and polysaccharide and has specific binding affinity to carbohydrates [30] It is widely distributed across plants, animals and microorganisms in nature [26]. Plant lectin is mainly isolated from dried seeds, but exists also in leaves, stems, roots, and tubers [39]. Particularly it is rich in seeds of leguminous plants among them [5,27].The lectin is synthesized in ribosome of plant cells, transferred to secretion system in form of glycoprotein, accumulated highly in vacuoles and cell walls [6], and then used in growth of young plants. In additions it serves...

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“…Cytotoxic activity of methanolic extract of donnani against leukemia were studied by Yalçın etal., [17]. Toxicity of lectins from sponges such as Axinella corrugata [18], Haliclona cratera [18], Cinachyrella apion [20], Cliona varians [21], Haliclona caerulea [22] against different cancer cell lines were reported earlier. Anyway, further studies were required are required to determine the mechanism of action of ADL on the antiproliferative activity of cancer cell lines studied.…”

Section: Fig1 Effect Of Denaturing Agents On Adlmentioning

confidence: 88%

Effect of denaturing agents on a lectin isolated from marine sponge Axinella donnaniand its potential cytotoxic activity

Sadanandan¹,

Rauf²

2023

J. Fundam and Appl Sci.

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Marine sponges are oldest metazoans which were found to have structural and biologically diverse lectins. A lectin isolated from marine sponge Axinella donnani (ADL) were evaluated for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Guanidine hydrochloride does not show any activity. Finally, cyt otoxicity of ADL at different concentrations (0.25 500 mg/mL) was tested against MCF 7, A431 and HeLa cell lines by MTT assay. ADL exhibited potential cytotoxicity against these cell lines after 24 hours in a dose dependent manner. Thus, ADL could be a pot ent cytotoxic agent in clinical applications.

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Immunohistochemical localization of an N-acetyl amino-carbohydrate specific lectin (ACL-I) of the marine sponge Axinella corrugata

Cited by 5 publications

References 19 publications

Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent

Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent

Comparison of Biochemical Characterization of Korean and Chinese Mung Bean Lectin

Effect of denaturing agents on a lectin isolated from marine sponge Axinella donnaniand its potential cytotoxic activity

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