Immunohistochemical localisation of urogastrone to human duodenal and submandibular glands.

Heitz, Philipp U.; Kasper, M.; Noorden, Susan Van; Polak, Julia M.; Gregory, H.; Pearse, A. G. E.

doi:10.1136/gut.19.5.408

Cited by 198 publications

(56 citation statements)

References 23 publications

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“…Human epidermal growth factor (EGF) is a potent mitogenic peptide produced by salivary and duodenal Brunner's glands, 4 which stimulates several components of the healing response. 5 Preliminary studies in humans suggest that topical EGF enhances healing of skin wounds 6 and that systemic EGF is beneficial for necrotizing enterocolitis in neonates.…”

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confidence: 99%

Epidermal Growth Factor Enemas with Oral Mesalamine for Mild-to-Moderate Left-Sided Ulcerative Colitis or Proctitis

et al. 2003

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Epidermal growth factor enemas with oral mesalamine for mild-tomoderate left-sided ulcerative colitis or proctitis Sinha, A; Nightingale, JMD; West, KP; Berlanga-Acosta, J; Playford, RJ For additional information about this publication click this link.https://qmro.qmul.ac.uk/jspui/handle/123456789/224 Information about this research object was correct at the time of download; we occasionally make corrections to records, please therefore check the published record when citing. For more information contact scholarlycommunications@qmul.ac.uk original articleThe new england journal of medicine n engl j med 349;4 www.nejm.org

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confidence: 99%

Epidermal Growth Factor Enemas with Oral Mesalamine for Mild-to-Moderate Left-Sided Ulcerative Colitis or Proctitis

et al. 2003

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“…A remarkably similar peptide, human urogastrone, has been isolated from human urine and subsequently shown to be synthesized in the duodenum and salivary glands (12). Human urogastrone is a potent inhibitor of gastric acid secretion and a promoter of epithelial cell proliferation (5,13).…”

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confidence: 99%

Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.

Urdea¹,

Merryweather²,

Mullenbach³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

102

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We have chemically synthesized and expressed in yeast a gene coding for human epidermal growth factor (urogastrone), a 53-amino-acid polypeptide that has been shown to promote epithelial cell proliferation and to inhibit gastric acid secretion. The synthetic gene, consisting of 170 base pairs, was designed with yeast-preferred codons and assembled by enzymatic ligation of synthetic fragments produced by phosphoramidite chemistry. The DNA synthesis protocol used allows for facile synthesis of oligonucleotides larger than 50 bases. Yeast cells were transformed with plasmids containing the synthetic gene under control of a yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter and were shown to synthesize a biologically active human epidermal growth factor.

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“…EGF is a very potent stimulator of cell proliferation in the rodent and human gastrointestinal mucosa (Walker-Smith et al 1985;Goodlad et al 1987), and also modulates intestinal epithelial cell di¡erentiation (Goodlad et al 1991). In normal conditions, EGF is produced by gut-associated salivary and Brunner's glands (Heitz et al 1978). There is evidence that EGF receptors in the gut are polarized to the laterobasal membranes (Scheving et al 1989).…”

Section: Gene Expression In the Uacl (A) Genes Related To Di¡erentiationmentioning

confidence: 99%

Aspects of the biology of regeneration and repair in the human gastrointestinal tract

Wright

1998

Phil. Trans. R. Soc. Lond. B

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The main pathways of epithelial di¡erentiation in the intestine, Paneth, mucous, endocrine and columnar cell lineages are well recognized. However, in abnormal circumstances, for example in mucosal ulceration, a cell lineage with features distinct from these emerges, which has often been dismissed in the past as pyloric' metaplasia, because of its morphological resemblance to the pyloric mucosa in the stomach. However, we can conclude that this cell lineage has a de¢ned phenotype unique in gastrointestinal epithelia, has a histogenesis that resembles that of Brunner's glands, but acquires a proliferative organization similar to that of the gastric gland. It expresses several peptides of particular interest, including epidermal growth factor, the trefoil peptides TFF1, TFF2, TFF3, lysozyme and PSTI. The presence of this lineage also appears to cause altered gene expression in adjacent indigenous cell lineages. We propose that this cell lineage is induced in gastrointestinal stem cells as a result of chronic mucosal ulceration, and plays an important part in ulcer healing; it should therefore be added to the repertoire of gastrointestinal stem cells.

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Immunohistochemical localisation of urogastrone to human duodenal and submandibular glands.

Cited by 198 publications

References 23 publications

Epidermal Growth Factor Enemas with Oral Mesalamine for Mild-to-Moderate Left-Sided Ulcerative Colitis or Proctitis

Epidermal Growth Factor Enemas with Oral Mesalamine for Mild-to-Moderate Left-Sided Ulcerative Colitis or Proctitis

Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.

Aspects of the biology of regeneration and repair in the human gastrointestinal tract

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