Immunohistochemical Features of O6-Methylguanine-DNA Methyltransferase Expression during Ovarian Endometriosis

Abstract

A comparative immunohistochemical study for the expression of O6-methylguanine-DNA methyltransferase (MGMT) was performed in tissues of the eutopic endometrium and ovarian endometriosis. The highest level of MGMT expression in eutopic endometrial tissue was observed in epitheliocyte nuclei during the proliferative phase. In regions of endometriosis the expression of MGMT in epitheliocyte nuclei was shown to increase during stages I and II, but decreased in stages III and IV. The progression of endometriosis was accompanied by a gradual increase of study parameters in the nuclei and cytoplasm of stromal cells. These changes reflect the impairment of DNA reparation, which probably serves as a stage in the development and progression of endometriosis.

Identification of MKNK1 and TOP3A as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments

“…S5 ). Notably, it has been reported that TPM2 , HOXB2 , and MGMT are associated with EM [37] , [38] , [39] . Based on our comprehensive integrative genomics analysis, we identified 14 genes, including 11 novel risk genes implicated in EM susceptibility ( Table 1 ).…”

“…To give an overview of significant loci obtained through GWAS, we summarized these reported significant variants in the Supplementary Table S10 . Among the 14 identified genes, TPM2 , HOXB2 , and MGMT have been shown to be associated with EM in a few previous studies [37] , [38] , [39] . Specifically, TPM2 encodes beta-tropomyosin, which plays a role in muscle contraction and motility, and helps maintain cell shape and cell-matrix interactions.…”

“…Vestergaard et al [38] observed that HOXB2 transcription was significantly reduced in EM lesions compared with endometrium samples from patients with EM and healthy controls, which was in agreement with our results. O6-methylguanine-DNA methyltransferase (MGMT), which is responsible for the direct repair of DNA, is primarily immunolocalised in the nuclei of epithelial cells in eutopic endometrial tissue and ovarian EM lesions [39] . Nevertheless, previous studies have only been focused on the investigation of the expression or localisation patterns of risk genes.…”