Immunohistochemical Expression of Markers of Immaturity in Sertoli and Seminal Cells in Canine Testicular Atrophy

Giudice, Chiara; Banco, Barbara; Veronesi, M.C.; Ferrari, Angelo; Nardo, Anna Di; Grieco, Valeria

doi:10.1016/j.jcpa.2013.08.001

Cited by 25 publications

(27 citation statements)

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“…Moreover, the expression of AMH in atrophic but not in mature Sertoli cells was reported previously (Giudice et al, 2014). In the light of these results, it seems to be promising to investigate further the use of AMH for diagnosis of gonadal atrophy.…”

Section: Amh As a Marker For Gonadal Atrophy In Malessupporting

confidence: 52%

Anti‐Müllerian hormone in dogs and cats reproduction

Walter¹

2020

Reprod Domestic Animals

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The anti-Müllerian hormone (AMH) is a glycoprotein secreted by Sertoli cells in males and granulosa cells in females. It has first been determined in blood serum of dogs and cats by Place et al. in 2011 with the use of a human-based ELISA test. Meanwhile, different immunoassays have been validated for AMH determination in animals and a variety of studies have demonstrated the clinical significance of AMH. This review summarizes the current knowledge about AMH in dogs and cats and describes future opportunities for its diagnostic use.

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Section: Amh As a Marker For Gonadal Atrophy In Malessupporting

confidence: 52%

Anti‐Müllerian hormone in dogs and cats reproduction

Walter¹

2020

Reprod Domestic Animals

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“…Similar results were obtained with NSE, which consistently labelled SCs in MGSCTs (Patnaik and Mostofi, 1993;Owston and RamosVara, 2007). CK, DES, INH-a and AMH, all reported as markers of immature SCs Banco et al, 2012), were not expressed in normal testes (Giudice et al, 2014). In contrast, these markers were expressed diffusely by neoplastic SCs within MGSCTs, consistent with neoplastic SCs in canine Sertoli cell tumours (SCTs) .…”

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confidence: 74%

“…Further sections (5 mm) were stained with PAS or subjected to immunohistochemistry (IHC) using the ABC method (Giudice et al, 2014). A panel of eight primary antibodies was applied including reagents specific for VIM (3B4, 1 in 1,000 dilution), INH-a (R1, 1 in 40 dilution), CK (AE1/AE3, 1 in 2,000 dilution), DES (NCL-L-DES-DERII, 1 in 300 dilution), AMH (C-20, 1 in 30,000 dilution), PLAP (8A9, 1 in 25 dilution), KIT (CD117, 1 in 500 dilution) and NSE (1 in 25 dilution).…”

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confidence: 99%

“…For each immunohistochemical test, a section of neonatal canine testes and a section of a canine cutaneous mast cell tumour were included as positive controls for AMH and KIT, respectively. For the other antibodies, internal positive controls were available and consisted of interstitial fibroblasts (VIM), normal and/or atrophic SCs (NSE), epithelial cells from rete testis and/or epididymis (CK), myoid peritubular cells (DES and PLAP) and Leydig cells (INH-a) (Giudice et al, 2014).…”

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confidence: 99%

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Immunohistochemical Study of Mixed Germ Cell Sex Cord Stromal Tumours in 13 Canine Testes

Banco

Giudice

Ghisleni

et al. 2015

Journal of Comparative Pathology

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“…AMH concentration did not differ significantly between control dogs and dogs with testicular tumours other than SCT or with NNP, but about half of dogs with these testicular changes had serum concentrations in the interval between 10 and 22 pg/mL. An increased expression of markers of immaturity in Sertoli cells, including AMH, has recently been described in cases of canine testicular atrophy [ 30 ]. Such changes may contribute to slightly elevated concentrations of AMH in some dogs with testicular pathologies other than SCT.…”

Section: Discussionmentioning

confidence: 99%

Anti-Müllerian hormone: a potentially useful biomarker for the diagnosis of canine Sertoli cell tumours

Holst

Dreimanis

2015

BMC Vet Res

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BackgroundTesticular tumours are common in dogs and in many cases do not give rise to clinical signs. In other cases, signs of feminization, hyperpigmentation or alopecia may be observed, most commonly associated with Sertoli cell tumours (SCT). Although these signs are often associated with elevated concentrations of oestradiol, analysis of oestradiol may give inconclusive results due to large variations among individuals. Other biomarkers are therefore needed. Anti-Müllerian hormone (AMH) is expressed by the Sertoli cell. In humans, AMH has been shown to be a specific marker of Sertoli cell origin in gonadal tumours. Using immunohistochemistry, AMH has been shown to be a useful marker of immature and neoplastic Sertoli cells in dogs. The aim of the present study was to evaluate the clinical relevance of AMH analysis in peripheral blood in the diagnostic workup of dogs with suspected testicular tumours.ResultsBlood was collected from 20 dogs with a palpable testicular mass and from 27 healthy controls. Serum was analysed for oestradiol-17β using a RIA and for AMH using an ELISA. The Mann–Whitney U test was used to compare hormone concentrations between different groups.All control dogs had AMH concentrations ≤ 10 ng/mL, except one outlier that had a concentration of 43 ng/mL. Six dogs with SCT or mixed tumours containing SCT had AMH concentrations higher than 22 ng/mL, significantly higher than AMH concentrations in control dogs (P = 0.0004). Concentrations between 10 and 22 ng/mL were found in about half of the dogs with non-neoplastic testicular pathologies or with testicular tumours other than SCTs. Age did not significantly affect concentrations of AMH in the control dogs.ConclusionAMH was shown to be a promising biomarker for the diagnosis of Sertoli cell tumours in dogs.

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Immunohistochemical Expression of Markers of Immaturity in Sertoli and Seminal Cells in Canine Testicular Atrophy

Cited by 25 publications

References 50 publications

Anti‐Müllerian hormone in dogs and cats reproduction

Anti‐Müllerian hormone in dogs and cats reproduction

Immunohistochemical Study of Mixed Germ Cell Sex Cord Stromal Tumours in 13 Canine Testes

Anti-Müllerian hormone: a potentially useful biomarker for the diagnosis of canine Sertoli cell tumours

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