Immunohistochemical Expression of Cortactin, E-Cadherin, and MDM2 Proteins in Solid Ameloblastoma versus Odontogenic Keratocyst (An immunohistochemical study)

Abstract

Purpose:The extent of invasion can be analyzed by the expression and production of various genes and proteins by lesional cells. However, current clinical parameters lack the potential to predict the neoplastic behavior in solid ameloblastoma (SAB) and odontogenic keratocyst (OKC). Cortactin, an F-actin binding protein, overexpression has been correlated with advanced clinic pathological stage and poor prognosis in several tumors. E-cadherin belongs to the classical cadherins which. Low E-cadherin expression correlated to aggressive, poorly differentiated, high-grade carcinomas and low patient survival. Human-murine double minute 2 (MDM2), contributes to the promotion of cell growth, survival, invasion, and therapeutic resistance, overexpression of MDM2 has been observed in various human cancers and can contribute to genomic instability, thus, further promoting tumorigenesis. The purpose of this work was to investigate the role of Cortactin, E-Cadherin, MDM2 proteins expression in SAB and OKC and correlate the expression of these markers with the aggressive behavior of these tumors. Material and method: 10 case of solid ameloblastoma with its different histologic variants and 10 cases of the keratocystic odontogenic tumor were collected as paraffin embedded blocks. An immunohistochemical investigation using, Cortactin, E-Cadherin, and MDM2 antibodieswere done for all specimens. Results: The mean area percent of immunoexpression of Cortactin was greatest in SAB while, the mean area percent of immunoexpression of E-Cadherin, MDM2 were greatest in OKC. Conculsion:According to the current study, the absence of any significant differences between AB and OKC indicate the neoplastic and aggressive nature of OKC similar to AB.

Comparative study of behavior between Conventional Ameloblastoma and Hybrid Ameloblastoma

“…More than 75% of stellate reticulum cells showed mainly membranous expression while 75% of the peripheral columnar cells showed negative expression. These findings were similar to those of Abd Elsamia et al, who reported that distribution of immune reaction for Ecadherin was noted on the cell membrane mainly in the central stellate reticulum-like cells than peripheral columnar cells of CMAM [17] . These findings were also in agreement with previous studies which demonstrated that the pattern of E-cadherin immunoreactivity was predominantly on the membrane at cell-cell borders and the most intense reactivity was noticed in the stellate-reticulum like cells.…”

“…Since the prognosis of various neoplasms including odontogenic tumors cannot be reliably and accurately predicted on the basis of clinical and histopathologic features only, it is highly desirable to find genetic markers to rely on. These genetic markers could be an objective measure to clarify alterations that might be occurring during the process of tumorigenesis [17] . Taking this into consideration, this study was designed to assess the immunohistochemical expression of E-cadherin and OPN in CMAM and HAM, correlate their expression to each other and to predict their prognostic value in these tumors.…”