Immunohistochemical Expression of ABH/Lewis-Related Antigens in Primary Breast Carcinomas and Metastatic Lymph Node Lesions

Abstract

The expression of blood group antigens A, B, and H, as well as sialylated and nonsialylated forms of Lewis(a) and Lewis(x), was studied using immunohistochemical methods in normal and tumor tissues in the following cohort of patients: 51 patients with primary breast carcinoma, 13 with metastatic lymph node lesions, and 16 with benign tumors of the breast. As a control, normal tissue was obtained from a similar group of 22 patients with breast cancer. The noncancerous tissues expressed the same A/B/H antigens as the patients' red blood cells and also usually expressed Lewis-related antigens. Seventy-six percent of primary carcinomas failed to express the appropriate A/B/H antigens, and in one blood group A patient the tumor tissue expressed B antigen. In the metastatic lesions, Lewis(a)/sialyl Lewis(a) expression was reduced when compared with the primary tumors, but Lewis(x)/sialyl Lewis(x) antigens were still expressed. These results suggest a possible relationship between the metastatic behavior of the tumor and expression of the blood group antigens.

Blood Type, Hormone Receptor Status, HER2/neu Status, and Survival in Breast Cancer: A Retrospective Study Exploring Relationships in a Phenotypically Well-Defined Cohort

“…Like loss of ABO expression in tissue, [8][9][10][11] HER2/neu positivity is a result of altered glycosylation leading to HER2/neu expression. 12 Over-expression of HER2/neu cell membrane receptor protein in breast carcinoma is associated with tumor with the lack of correlation between blood type and HER2/ neu status noted in the present study.…”

Different Expression of Sialyl Tn Antigen between Polypoid and Nonpolypoid Growth Types of Advanced Colorectal Carcinoma

“…Indirect immunoperoxidase staining was accomplished as follows [22]: monoclonal antibody TKH2 was diluted 1:10 in 0.05 M Tris, 2.5% bovine serum albumin, mouse liver acetone powder (100 mg/l; Sigma, St. Louis, Mo., USA) and 0.05% sodium azide. After treatment with 3% hydrogen peroxide in 0.05 M Tris-buffered saline (TBS; 50 m M Tris-HCl 200 m M NaCl, pH 7.6) for 5 min, the monoclonal antibody was incubated with tissue sections for 1 h at room temperature in a moist chamber.…”

Expression of blood group antigens A, B and H in carcinoma tissue correlates with a poor prognosis for colorectal cancer patients

“…Immunohistochemistry was performed as previously described, using an indirect immunoperoxidase staining technique (Nakagoe et al 1998). All steps were conducted at room temperature.…”

“…Tumors or tissues with more than 5% stained cells were de®ned as positively stained and those with 5% or less as negatively stained (Nakagoe et al 1998). Two of the authors judged the results of immunohistochemical staining independently, and the ®nal result was assigned by consensus.…”