Immunohistochemical evidence of the presence of metabotropic receptors for γ-aminobutyric acid at the rat neuromuscular junctions

Malomuzh, A. I.; Нуруллин, Л. Ф.; Nikolsky, E. E.

doi:10.1134/s1607672915040092

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…The degree of picrotoxin‐induced facilitation of muscle contractility was also dependent on the frequency of motor nerve stimulation; that is, any effect of picrotoxin at 10 and 20 Hz was absent, and it enhanced the force of contractions at 50 and 70 Hz (by 18 and 37%, respectively). Thus, the observed effects of CGP 55845 and picrotoxin support the idea of expression of functional GABA B receptors (Glavinović, ; Malomouzh et al., ; Malomuzh et al., ) and GABA A receptors (Chiou & Chang, ) at the mammalian neuromuscular junction.…”

Section: Discussionsupporting

confidence: 68%

“…Thus, the observed effects of CGP 55845 and picrotoxin support the idea of expression of functional GABA B receptors (Glavinović, 1979;Malomouzh et al, 2015;Malomuzh et al, 2015) and GABA A receptors (Chiou & Chang, 1994) at the mammalian neuromuscular junction.…”

Section: Discussionsupporting

confidence: 66%

“…Recently, using immunohistochemistry, we have detected GABA molecules, L-glutamate decarboxylase and GABA transporter in the region of the rat neuromuscular junction (Nurullin, Nikolsky, & Malomouzh, 2018). We also have detected metabotropic GABA B receptors (Malomuzh, Nurullin, & Nikolsky, 2015). Using electrophysiological methods, we have shown that activation of GABA B receptors by an exogenous agonist caused a reduction in the evoked acetylcholine release from the motor nerve ending (Malomouzh, Petrov, Nurullin, & Nikolsky, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors

Lenina

Петров

Kovyazina

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?Do GABA receptors play any role at the neuromuscular junction? What is the main finding and its importance?In the presence of either ionotropic or metabotropic GABA receptor antagonists, diaphragm muscle force production elicited by stimulating the motor nerve at ≥50 Hz was increased. Our data indicate the presence of GABAergic signalling at the neuromuscular junction. Abstract Despite the signalling role of GABA in the brain and spinal cord, the role of this molecule in the peripheral nervous system and, in particular, at the neuromuscular junction remains practically unexplored. In the present work, the force of mouse diaphragm contractions was measured in the presence of blockers of metabotropic GABAB receptors (CGP 55845) and ionotropic GABAA receptors (picrotoxin) with various patterns of indirect and direct stimulation of muscle by trains of 40 pulses delivered at 10, 20, 50 and 70 Hz. It was found that neither blocker affected the diaphragm contractility caused by indirect stimulation through the motor nerve at 10 and 20 Hz. However, when the stimulation frequency was increased to 50 or 70 Hz, the force of subsequent contractions in the train (when compared with the amplitude of contraction in response to the first pulse) was increased by both CGP 55845 and picrotoxin. With direct stimulation of the diaphragm, no significant changes in the contraction force were detected at any frequency used. The results obtained support the following conclusions: (i) pharmacological inhibition of GABA receptors increases the contractile activity of skeletal muscle; and (ii) frequency‐dependent enhancement of GABA receptor activation takes place in the region of the neuromuscular junction.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors

Lenina

Петров

Kovyazina

et al. 2019

Experimental Physiology

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“…The NMJ is a chemical-type synapse in which transmission of basic signals is mediated by acetylcholine (ACh). However, it is worth mentioning that other neurotransmitters (glutamate, ATP, GABA) have been shown to exist in this presumably cholinergic synapse, whose putative role is fine-tuning of ACh release [3, 4, 5].…”

Section: Introductionmentioning

confidence: 99%

Influence of the Activation of NMDA Receptors on the Resting Membrane Potential of the Postsynaptic Cell at the Neuromuscular Junction

2018

Self Cite

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Impaired function or insufficient expression of glutamate N-methyl-D-aspartate (NMDA) receptors underlies a number of brain pathologies; these receptors are, therefore, regarded as a pharmacological target for many neuroactive drugs. It was shown that in the CNS, this type of glutamate receptors participate in the processes of neuronal excitation, synaptic plasticity [1, 2], and excitotoxicity in neurodegenerative diseases and are also involved in the pathogenesis of epilepsy and seizures. However, until recently, the presence and activity of NMDA receptors beyond the CNS had never been considered. This research shows that activation of NMDA receptors at the mammalian neuromuscular junction alters the resting membrane potential of the postsynaptic cell evoked by cation entry through the receptor-associated channel.

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GABA in developing rat skeletal muscle and motor neurons

Sibgatullina

Malomouzh

2020

Protoplasma

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Immunohistochemical evidence of the presence of metabotropic receptors for γ-aminobutyric acid at the rat neuromuscular junctions

Cited by 5 publications

References 13 publications

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors

Influence of the Activation of NMDA Receptors on the Resting Membrane Potential of the Postsynaptic Cell at the Neuromuscular Junction

GABA in developing rat skeletal muscle and motor neurons

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Immunohistochemical evidence of the presence of metabotropic receptors for γ-aminobutyric acid at the rat neuromuscular junctions

Cited by 5 publications

References 13 publications

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABAA and GABAB receptors

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABAA and GABAB receptors

Influence of the Activation of NMDA Receptors on the Resting Membrane Potential of the Postsynaptic Cell at the Neuromuscular Junction

GABA in developing rat skeletal muscle and motor neurons

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Product

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Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors

Enhancement of mouse diaphragm contractility in the presence of antagonists of GABA_A and GABA_B receptors