GABA in developing rat skeletal muscle and motor neurons

Sibgatullina, Guzel; Malomouzh, A. I.

doi:10.1007/s00709-020-01485-1

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…In turn, polyamine oxidases, as participants in the metabolism of polyamines, can participate in the implementation of an alternative pathway for the synthesis of GABA [Tillakaratne et al, 1995]. And since we previously found that this amino acid in the culture of myocytes and myotubes is not synthesized in a "classical" way (with the participation of L-glutamate decarboxylase) but is formed in some alternative way [Sibgatullina and Malomouzh, 2020], it is likely that a decrease in the GABA content in muscle elements growing in the differentiation medium is caused precisely by the deficiency of polyamine oxidase. The direct participation of GABA molecules in the regulation of the development of non-neuronal tissue has been identified for a long time.…”

Section: Discussionmentioning

confidence: 99%

“…We recently found the presence of GABA molecules directly in cultured myocytes and myotubes, as well as in the skeletal muscle of newborn rats [Sibgatullina and Malomouzh, 2020]. The reduction of the GABA content in the muscle fiber during ontogenesis and its complete absence in the mature tissue [Sibgatullina and Malomouzh, 2020] allows to suggest the participation or influence (direct or indirect) of GABA on certain processes (the division of myocytes and/or their fusion into myotubes) in early myogenesis. This assumption predetermined the present study.…”

Section: Introductionmentioning

confidence: 98%

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Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid

Sibgatullina¹,

Ebrahim²,

Gilizhdinova³

et al. 2023

Cells Tissues Organs

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There are many facts about the possible role of gamma-aminobutyric acid (GABA) in the development and differentiation of cells not only in nervous but also in muscle tissue. In the present study a primary culture of rat skeletal muscle myocytes was used to evaluate the correlation between the content of GABA in the cytoplasm and the processes of myocyte division and their fusion into myotubes.The effect of exogenous GABA on the processes of culture development was also estimated. Since the classical protocol for working with myocyte cultures involves the use of fetal bovine serum (FBS) to stimulate cell division (growth medium) and horse serum (HS) to activate the differentiation process (differentiation medium), the studies were carried out both in the medium with FBS and with HS. It was found that cells grown in medium supplemented withFBS contain more GABA compared to cultures growing in medium supplemented with HS. Addition of exogeneous GABA leads to a decrease in the number of myotubes formed in both media, while the addition of an amino acid to the medium supplemented with HS had a more pronounced inhibitory effect. Thus, we have obtained data indicating that GABA is able to participate in the early stages of skeletal muscle myogenesis by modulating the fusion process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid

Sibgatullina¹,

Ebrahim²,

Gilizhdinova³

et al. 2023

Cells Tissues Organs

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“…The degree of colocalization of liposomes with mitochondria was determined using a primary culture of rat motoneurons prepared as described by Sibgatullina and Malomouzh [ 110 ]. The cells were seeded in 24 × 24 mm glass plates.…”

Section: Methodsmentioning

confidence: 99%

Mitochondria-Targeted Delivery Strategy of Dual-Loaded Liposomes for Alzheimer’s Disease Therapy

Васильева

Gaynanova

Валеева

et al. 2023

IJMS

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Liposomes modified with tetradecyltriphenylphosphonium bromide with dual loading of α-tocopherol and donepezil hydrochloride were successfully designed for intranasal administration. Physicochemical characteristics of cationic liposomes such as the hydrodynamic diameter, zeta potential, and polydispersity index were within the range from 105 to 115 nm, from +10 to +23 mV, and from 0.1 to 0.2, respectively. In vitro release curves of donepezil hydrochloride were analyzed using the Korsmeyer–Peppas, Higuchi, First-Order, and Zero-Order kinetic models. Nanocontainers modified with cationic surfactant statistically better penetrate into the mitochondria of rat motoneurons. Imaging of rat brain slices revealed the penetration of nanocarriers into the brain. Experiments on transgenic mice with an Alzheimer’s disease model (APP/PS1) demonstrated that the intranasal administration of liposomes within 21 days resulted in enhanced learning abilities and a reduction in the formation rate of Aβ plaques in the entorhinal cortex and hippocampus of the brain.

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Increased endocytosis rate and enhanced lysosomal pathway of silica-coated superparamagnetic nanoparticles into M-HeLa cells compared with cultured primary motor neurons

Sibgatullina,

Ramazanova,

Salnikov

et al. 2024

Histochem Cell Biol

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GABA in developing rat skeletal muscle and motor neurons

Cited by 8 publications

References 32 publications

Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid

Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid

Mitochondria-Targeted Delivery Strategy of Dual-Loaded Liposomes for Alzheimer’s Disease Therapy

Increased endocytosis rate and enhanced lysosomal pathway of silica-coated superparamagnetic nanoparticles into M-HeLa cells compared with cultured primary motor neurons

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